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Literature summary for 2.6.1.72 extracted from

  • Rojanarata, T.; Laiwattanapaisal, W.; Ngawhirunpat, T.; Opanasopit, P.; Worrawethanakul, K.; Chantadee, T.; Fuangwattana, T.
    Fast, affordable and eco-friendly enzyme kinetic method for the assay of alpha-ketoglutaric acid in medical product and sports supplements (2018), Enzyme Microb. Technol., 116, 72-76.
    View publication on PubMed
Search for more literature for 2.6.1.72

Application

Application Comment Organism
analysis development of a simple, inexpensive, accurate and precise kinetic method for the quantitation of 2-oxoglutarate (AKG) in cardioplegic solution and athletic supplements. The assay relies on an enzymatic transamination of AKG and D-4-hydroxyphenylglycine to form 4-hydroxybenzoylformate and L-glutamate using D-phenylglycine aminotransferase. The method shows good linearity (r2 = 0.9994) over an AKG concentration range of 0.020-0.160 mM. The limits of detection and quantitation are 0.00409 and 0.01362 mM, respectively. The detection is not interfered with by excipients in the samples Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-4-hydroxyphenylglycine + 2-oxoglutarate Pseudomonas putida
-
 4-hydroxyphenylglyoxylate + L-glutamate
-
 ?
D-phenylglycine + 2-oxoglutarate Pseudomonas putida
-
 phenylglyoxylate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-4-hydroxyphenylglycine + 2-oxoglutarate
-
 Pseudomonas putida 4-hydroxyphenylglyoxylate + L-glutamate
-
 ?
D-phenylglycine + 2-oxoglutarate
-
 Pseudomonas putida phenylglyoxylate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
D-phenylglycine aminotransferase
-
 Pseudomonas putida
D-PhgAT
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas putida