Application | Comment | Organism |
---|---|---|
analysis | development of a simple, inexpensive, accurate and precise kinetic method for the quantitation of 2-oxoglutarate (AKG) in cardioplegic solution and athletic supplements. The assay relies on an enzymatic transamination of AKG and D-4-hydroxyphenylglycine to form 4-hydroxybenzoylformate and L-glutamate using D-phenylglycine aminotransferase. The method shows good linearity (r2 = 0.9994) over an AKG concentration range of 0.020-0.160 mM. The limits of detection and quantitation are 0.00409 and 0.01362 mM, respectively. The detection is not interfered with by excipients in the samples | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-4-hydroxyphenylglycine + 2-oxoglutarate | Pseudomonas putida | - |
4-hydroxyphenylglyoxylate + L-glutamate | - |
? | |
D-phenylglycine + 2-oxoglutarate | Pseudomonas putida | - |
phenylglyoxylate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-4-hydroxyphenylglycine + 2-oxoglutarate | - |
Pseudomonas putida | 4-hydroxyphenylglyoxylate + L-glutamate | - |
? | |
D-phenylglycine + 2-oxoglutarate | - |
Pseudomonas putida | phenylglyoxylate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-phenylglycine aminotransferase | - |
Pseudomonas putida |
D-PhgAT | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |