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Literature summary for 2.6.1.72 extracted from

  • Jariyachawalid, K.; Laowanapiban, P.; Meevootisom, V.; Wiyakrutta, S.
    Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES (2012), Microb. Cell Fact., 11, 47.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis functional expression in Pichia pastoris. Co-expression of Escherichia coli chaperonins GroEL-GroES with the enzyme gene dramatically improves the soluble active enzyme production. Increasing gene dosage of both the enzyme and those of the chaperones further increases functional protein yield up to 14400fold higher than when the enzyme is expressed alone. Optimization of cultivation condition further increases activity yield from the best co-expressing strain by 1.2fold Pseudomonas stutzeri

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri Q6VY99
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Pseudomonas stutzeri ST201 Q6VY99
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Synonyms

Synonyms Comment Organism
D-PhgAT
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 Pseudomonas stutzeri
dpgA
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 Pseudomonas stutzeri