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Literature summary for 2.6.1.7 extracted from

  • Yang, C.; Zhang, L.; Han, Q.; Liao, C.; Lan, J.; Ding, H.; Zhou, H.; Diao, X.; Li, J.
    Kynurenine aminotransferase 3/glutamine transaminase L/cysteine conjugate beta-lyase 2 is a major glutamine transaminase in the mouse kidney (2016), Biochem. Biophys. Rep., 8, 234-241 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.7

Application

Application Comment Organism
analysis the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
recombinant His-tagged enzyme expression in Escherichia coli, enzyme expression analysis by realtime RT-PCR Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 phenylpyruvate recombinant enzyme, pH 7.5, 38°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-
liver
-
 Mus musculus
-
additional information tissue localizations of KAT3/GTL/CCBL2, real-time RT-PCR enzyme expression analysis in tissues of female and male animals, overview. The enzyme is present in both liver and kidney of the mouse, but is much more abundant in the kidney than in the liver Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-kynurenine + 2-oxobutyrate high activity Mus musculus ?
-
 ?
L-kynurenine + 2-oxocaproate high activity, 2-oxocaproate is the best cosubstrate Mus musculus ?
-
 ?
L-kynurenine + 2-oxoglutarate very low activity with 2-oxogltarate Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
-
 ?
L-kynurenine + 2-oxoleucine moderate activity Mus musculus ?
-
 ?
L-kynurenine + 2-oxovalerate high activity Mus musculus ?
-
 ?
L-kynurenine + 2-oxovaline low activity Mus musculus ?
-
 ?
L-kynurenine + glyoxylate high activity Mus musculus ?
-
 ?
L-kynurenine + hydroxyphenylpyruvate moderate activity Mus musculus ?
-
 ?
L-kynurenine + indol-3-pyruvate moderate activity Mus musculus ?
-
 ?
L-kynurenine + mercaptopyruvate moderate activity Mus musculus ?
-
 ?
L-kynurenine + oxaloacetate moderate activity Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-aspartate
-
 ?
L-kynurenine + phenylpyruvate high activity Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-phenylalanine
-
 ?
L-kynurenine + pyruvate moderate activity Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 ?
additional information cosubstrate specificity, overview. No or poor activity with 2-oxoadipate and 2-oxoisoleucine. Recombinant mouse KAT3/GTL selectively metabolizes selenomethionine to 2-oxo-gamma-methylselenobutyrate, KAT3/GTLis more efficient in transamination of both than is KAT1/GTK Mus musculus ?
-
-

Synonyms

Synonyms Comment Organism
KAT3
-
 Mus musculus
KAT3/GTL/CCBL2
-
 Mus musculus
kynurenine aminotransferase 3
-
 Mus musculus
More see also EC 2.6.1.64 and EC 4.4.1.13 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.17
-
 phenylpyruvate recombinant enzyme, pH 7.5, 38°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mus musculus

General Information

General Information Comment Organism
evolution kynurenine aminotransferase 3 (KAT3, EC 2.6.1.7) catalyzes the transamination of kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 (CCBL2, EC 4.4.1.13) and glutamine transaminase L (GTL, EC 2.6.1.64) Mus musculus
additional information the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney Mus musculus
physiological function kynurenine aminotransferase 3 (KAT3) catalyzes the transamination of kynurenine to kynurenic acid Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.28
-
 phenylpyruvate recombinant enzyme, pH 7.5, 38°C Mus musculus