Cloned (Comment) | Organism |
---|---|
tagged protein (consisting of an intein and a chitin binding domain) expressed in Escherichia coli | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aspartate | - |
Mus musculus | |
cysteine | - |
Mus musculus | |
glutamate | - |
Mus musculus | |
glutamine | - |
Mus musculus | |
histidine | - |
Mus musculus | |
Indole-3-propionic acid | - |
Mus musculus | |
leucine | - |
Mus musculus | |
methionine | - |
Mus musculus | |
phenylalanine | - |
Mus musculus | |
tryptophan | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P05202 | - |
- |
Mus musculus | Q71RI9 | - |
- |
Mus musculus | Q8BTY1 | - |
- |
Mus musculus | Q9WVM8 | - |
- |
Purification (Comment) | Organism |
---|---|
chitin affinity chromatography, cleavage of tag domain, ion exchange chromatography (DEAE-Sepharose and Mono-Q), gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
freezing and thawing do not alter the activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + 2-oxoglutarate | - |
Mus musculus | kynurenic acid + L-glutamate + H2O | - |
? | |
L-kynurenine + 2-oxoglutarate | highest activity with aspartate and glutamate as substrate, but also active with other amino acids | Mus musculus | kynurenic acid + L-glutamate + H2O | - |
? | |
L-kynurenine + glyoxylate | 2-oxobutyrate is also a co-substrate | Mus musculus | kynurenic acid + glycine + H2O | - |
? | |
L-kynurenine + glyoxylate | alpha-ketobutyrate is also a co-substrate | Mus musculus | kynurenic acid + glycine + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminoadipate aminotransferase | - |
Mus musculus |
cysteine conjugate beta-lyase 1 | - |
Mus musculus |
cysteine conjugate beta-lyase 2 | - |
Mus musculus |
glutamic-oxaloacetic transaminase 2 | - |
Mus musculus |
glutamine transaminase K | - |
Mus musculus |
kynurenine aminotransferase I | - |
Mus musculus |
kynurenine aminotransferase II | - |
Mus musculus |
kynurenine aminotransferase III | - |
Mus musculus |
kynurenine aminotransferase IV | - |
Mus musculus |
mitochondrial aspartate aminotransferase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
59 | - |
- |
Mus musculus |
60 | - |
- |
Mus musculus |
60 | 70 | - |
Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
midpoint temperature of protein melting 64.8°C, calculated half life time value at 65°C 3.9 min | Mus musculus |
additional information | - |
midpoint temperature of protein melting 65.9°C, calculated half life time value at 65°C 27.4 min | Mus musculus |
additional information | - |
midpoint temperature of protein melting 66.5°C, calculated half life time value at 65°C 6.5 min | Mus musculus |
additional information | - |
midpoint temperature of protein melting 68.8°C, calculated half life time value at 65°C 69.7 min | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Mus musculus |
8 | - |
- |
Mus musculus |
9 | - |
- |
Mus musculus |