Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
in complex with L-kynurenine and L-glutamine, hanging drop vapor diffusion method, using 21% (w/v) polyethylene glycol 400, 10% glycerol, 150 mM CaCl2, and 100 mM HEPES, pH 7.5 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-hydroxykynurenine | - |
Mus musculus | |
L-cysteine | - |
Mus musculus | |
L-glutamine | - |
Mus musculus | |
L-histidine | - |
Mus musculus | |
L-leucine | - |
Mus musculus | |
L-methionine | - |
Mus musculus | |
additional information | indol-3ylpyruvate shows no noticeable inhibition of KAT III | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
glyoxylate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.4 | - |
alpha-oxo-gamma-methiol-butyric acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.5 | - |
indo-3-pyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.5 | - |
2-oxocaproic acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.6 | - |
phenylpyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.7 | - |
L-cysteine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.7 | - |
L-glutamine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.7 | - |
L-histidine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.9 | - |
L-methionine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1 | - |
2-oxobutyrate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1 | - |
hydroxyphenylpyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.1 | - |
L-phenylalanine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.2 | - |
2-oxovalerate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.4 | - |
L-asparagine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.5 | - |
L-kynurenine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.4 | - |
Mercaptopyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.7 | - |
L-tyrosine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
3 | - |
L-serine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
4.9 | - |
oxaloacetate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
5.3 | - |
2-oxoisocaproic acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
6.2 | - |
L-alanine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
7.1 | - |
L-tryptophan | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
8.1 | - |
2-oxoglutarate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
10.6 | - |
pyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q71RI9 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography, hydroxyapatite column chromatography, Mono-Q column chromatography, and gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobutyrate + glyoxylate | - |
Mus musculus | ? | - |
? | |
3-hydroxykynurenine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-alanine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-asparagine + glyoxylate | KAT II has high transamination activity toward L-asparagine | Mus musculus | ? | - |
? | |
L-cysteine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-glutamine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-histidine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-kynurenine + 2-oxobutyrate | - |
Mus musculus | kynurenic acid + 2-aminobutanoate + H2O | - |
? | |
L-kynurenine + 2-oxocaproic acid | - |
Mus musculus | ? | - |
? | |
L-kynurenine + 2-oxoglutarate | poor cosubstrate for KAT III | Mus musculus | ? | - |
? | |
L-kynurenine + 2-oxoisocaproic acid | - |
Mus musculus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-leucine | - |
? | |
L-kynurenine + 2-oxomethylthiobutyric acid | - |
Mus musculus | ? | - |
? | |
L-kynurenine + 2-oxovalerate | - |
Mus musculus | ? | - |
? | |
L-kynurenine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-kynurenine + indol-3-ylpyruvate | - |
Mus musculus | ? | - |
? | |
L-kynurenine + mercaptopyruvate | - |
Mus musculus | ? | - |
? | |
L-kynurenine + oxaloacetate | - |
Mus musculus | ? | - |
? | |
L-kynurenine + p-hydroxyphenylpyruvate | - |
Mus musculus | ? | - |
? | |
L-kynurenine + phenylpyruvate | - |
Mus musculus | ? | - |
? | |
L-kynurenine + phenylpyruvate | poor cosubstrate for KAT III | Mus musculus | ? | - |
? | |
L-kynurenine + pyruvate | poor cosubstrate for KAT III | Mus musculus | ? | - |
? | |
L-lysine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-methionine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-phenylalanine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-serine + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-tryptophan + glyoxylate | - |
Mus musculus | ? | - |
? | |
L-tyrosine + glyoxylate | - |
Mus musculus | ? | - |
? | |
additional information | KAT III does not use L-valine, L-proline, L-threonine, L-leucine, L-isoleucine, L-glutamate, L-aspartate, L-arginine, and aminoadipate as substrates | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KAT III | - |
Mus musculus |
kynurenine aminotransferase III | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Mus musculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
2-oxoglutarate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.74 | - |
2-oxoisocaproic acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.02 | - |
alpha-oxo-gamma-methiol-butyric acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.03 | - |
L-tyrosine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.03 | - |
indo-3-pyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.3 | - |
L-cysteine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.48 | - |
hydroxyphenylpyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.53 | - |
L-alanine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.87 | - |
pyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2 | - |
L-histidine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.17 | - |
L-serine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.27 | - |
L-glutamine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.3 | - |
L-kynurenine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.43 | - |
L-methionine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.53 | - |
phenylpyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.59 | - |
2-oxocaproic acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.7 | - |
L-phenylalanine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.7 | - |
2-oxovalerate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.71 | - |
glyoxylate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.93 | - |
L-asparagine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.93 | - |
2-oxobutyrate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
3.28 | - |
Mercaptopyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
3.57 | - |
L-tryptophan | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
3.68 | - |
oxaloacetate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
2-oxoglutarate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.14 | - |
2-oxoisocaproic acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.18 | - |
pyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.25 | - |
L-alanine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.38 | - |
L-tyrosine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.5 | - |
L-tryptophan | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.72 | - |
L-serine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
0.75 | - |
oxaloacetate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.37 | - |
Mercaptopyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.48 | - |
hydroxyphenylpyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.53 | - |
L-kynurenine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
1.86 | - |
L-cysteine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.07 | - |
indo-3-pyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.095 | - |
L-asparagine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.25 | - |
2-oxovalerate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.45 | - |
L-phenylalanine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.54 | - |
alpha-oxo-gamma-methiol-butyric acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.7 | - |
L-methionine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.86 | - |
L-histidine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
2.93 | - |
2-oxobutyrate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
3.24 | - |
L-glutamine | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
4.22 | - |
phenylpyruvate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
5.18 | - |
2-oxocaproic acid | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus | |
6.78 | - |
glyoxylate | in 100 mM boric acid buffer, pH 9.0, at 45°C | Mus musculus |