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Literature summary for 2.6.1.7 extracted from

  • Tanizawa, K.; Asada, Y.; Soda, K.
    L-Kynurenine transaminase from Hansenula schneggii (1985), Methods Enzymol., 113, 90-95.
    View publication on PubMed
Search for more literature for 2.6.1.7

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Hansenula schneggii

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate
-
 Hansenula schneggii
Dicarboxylic acids
-
 Hansenula schneggii
Hg2+
-
 Hansenula schneggii
iodoacetate
-
 Hansenula schneggii
additional information not inhibited by EDTA, 2,2'-dipyridyl Hansenula schneggii
N-ethylmaleimide
-
 Hansenula schneggii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 2-oxoisocaproate
-
 Hansenula schneggii
1.1
-
 2-oxoglutarate
-
 Hansenula schneggii
1.2
-
 beta-phenylpyruvate
-
 Hansenula schneggii
2.6
-
 L-tryptophan
-
 Hansenula schneggii
5
-
 L-methionine
-
 Hansenula schneggii
5
-
 L-leucine
-
 Hansenula schneggii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 sedimentation equilibrium Hansenula schneggii
102000
-
 native PAGE Hansenula schneggii
106000
-
 gel filtration Hansenula schneggii

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
 low activity
-
Debaryomyces hansenii
-
 yeast
-
Diutina rugosa
-
 low activity
-
Hansenula schneggii
-
 yeast
-
Kluyveromyces marxianus
-
-
-
Naganishia albida
-
 low activity
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces fragilis
-
 low activity
-
Schwanniomyces occidentalis
-
 low activity
-
Schwanniomyces polymorphus
-
 yeast
-
Torulaspora globosa
-
 low activity
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hansenula schneggii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.9
-
-
 Hansenula schneggii

Storage Stability

Storage Stability Organism
-20°C, at protein concentrations above 0.6 mg/ml, 0.02 mM pyridoxal phosphate, 0.01% mercaptoethanol, several weeks, no loss of activity Hansenula schneggii
4°C, crystalline enzyme as suspension in 70% saturated ammonium sulfate, pH 7.2, more than 6 months Hansenula schneggii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminohexane-1,6-dioate + 2-oxoglutarate at about 60% the rate of L-kynurenine Hansenula schneggii 2-oxohexane-1,6-dioate + L-glutamate
-
 ?
L-3-hydroxykynurenine + 2-oxoglutarate at about 45% the rate of L-kynurenine Hansenula schneggii xanthurenic acid + L-glutamate
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Saccharomyces fragilis kynurenic acid + L-glutamate + H2O
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Cutaneotrichosporon cutaneum kynurenic acid + L-glutamate + H2O
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Schwanniomyces occidentalis kynurenic acid + L-glutamate + H2O
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Diutina rugosa kynurenic acid + L-glutamate + H2O
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Naganishia albida kynurenic acid + L-glutamate + H2O
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Torulaspora globosa kynurenic acid + L-glutamate + H2O
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Saccharomyces cerevisiae kynurenic acid + L-glutamate + H2O via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid ?
L-kynurenine + 2-oxoglutarate
-
 Kluyveromyces marxianus kynurenic acid + L-glutamate + H2O via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid ?
L-kynurenine + 2-oxoglutarate
-
 Debaryomyces hansenii kynurenic acid + L-glutamate + H2O via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid ?
L-kynurenine + 2-oxoglutarate
-
 Schwanniomyces polymorphus kynurenic acid + L-glutamate + H2O via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid ?
L-kynurenine + 2-oxoglutarate best substrates Hansenula schneggii kynurenic acid + L-glutamate + H2O via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid ?
L-kynurenine + 2-oxohexanedioate
-
 Hansenula schneggii kynurenic acid + 2-aminohexanedioate + H2O
-
 ?
L-kynurenine + 2-oxoisohexanoate
-
 Hansenula schneggii kynurenic acid + 2-aminoisohexanoate + H2O
-
 ?
L-kynurenine + 3-indole-2-oxopropanoate
-
 Hansenula schneggii kynurenic acid + L-tryptophan + H2O
-
 ?
L-kynurenine + 3-phenyl-2-oxopropanoate
-
 Hansenula schneggii kynurenic acid + L-phenylalanine + H2O
-
 ?
L-kynurenine + 4-methylsulfanyl-2-oxobutyrate
-
 Hansenula schneggii kynurenic acid + L-methionine + H2O
-
 ?
L-leucine + 2-oxoglutarate at about 85% the rate of L-kynurenine Hansenula schneggii 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-methionine + 2-oxoglutarate at about 36% the rate of L-kynurenine Hansenula schneggii 4-methylsulfanyl-2-oxobutanoate + glutamate
-
 ?
L-tryptophan + 2-oxoglutarate at about 60% the rate of L-kynurenine Hansenula schneggii 3-indole-2-oxopropanoate + L-glutamate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Hansenula schneggii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 and below, 5 min stable between pH 6.0 and pH 10.0 Hansenula schneggii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Hansenula schneggii