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Literature summary for 2.6.1.7 extracted from

  • Alberati-Giani, D.; Malherbe, P.; Köhler, C.; Lang, G.; Kiefer, V.; Lahm, H.W.; Cesura, A.M.
    Cloning and characterization of a soluble kynurenine aminotransferase from rat brain: identity with kidney cysteine conjugate beta-lyase (1995), J. Neurochem., 64, 1448-1455.
    View publication on PubMed
Search for more literature for 2.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
transient expression in HEK-293 cells Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
 L-kynurenine pH 8.0, 37°C, recombinant enzyme, cosubstrate 2-oxoglutarate Rattus norvegicus
1.2
-
 L-kynurenine pH 8.0, 37°C, recombinant enzyme, cosubstrate pyruvate Rattus norvegicus
1.7
-
 L-kynurenine pH 8.0, 37°C, cosubstrate pyruvate Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
soluble
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, SDS-PAGE Rattus norvegicus
90000 100000 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 identical to kidney soluble cysteine conjugate beta-lyase also referred to as glutamine transaminase K, K stands for kidney
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, phenyl-Sepharose, Superose 12, Mono Q Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.36
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-kynurenine + 2-oxoglutarate soluble kidney/brain kynurenine aminotransferase has both amino transferase and cysteine conjugate beta-lyase, i.e., glutamine transaminase K activities Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
 ?
L-kynurenine + pyruvate 100fold higher maximal velocity than with 2-oxoglutarate Rattus norvegicus kynurenic acid + L-alanine + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
KAT
-
 Rattus norvegicus
More identical to kidney soluble cysteine conjugate beta-lyase, EC 2.6.1.64, also referred to as glutamine transaminase K, EC 2.6.1.15, K stands for kidney Rattus norvegicus