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Literature summary for 2.6.1.67 extracted from

  • Der Garabedian, P.A.; Vermeersch, J.J.
    Lysine degradation in Candida. Characterization and probable role of L-norleucine-leucine, 4-aminobutyrate and delta-aminovalerate:2-oxoglutarate aminotransferases (1989), Biochimie, 71, 497-503.
    View publication on PubMed
Search for more literature for 2.6.1.67

Activating Compound

Activating Compound Comment Organism Structure
L-lysine this enzyme is strongly induced in vivo by growing Candida on L-lysine Candida guilliermondii var. membranaefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-leucine + 2-oxoglutarate Candida guilliermondii var. membranaefaciens
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-norleucine + 2-oxoglutarate Candida guilliermondii var. membranaefaciens
-
 2-oxohexanoate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Candida guilliermondii var. membranaefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate partial purification and several chromatographic steps, all steps at 4°C Candida guilliermondii var. membranaefaciens

Storage Stability

Storage Stability Organism
-25°C, stable in the presence of 0.01 mM pyridoxal 5'-phosphate and 0.01 mM 2-oxoglutarate Candida guilliermondii var. membranaefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-N-methylornithine + 2-oxoglutarate
-
 Candida guilliermondii var. membranaefaciens 5-(methylamino)-2-oxopentanoic acid + L-glutamate
-
 ?
6-N-acetyllysine + 2-oxoglutarate
-
 Candida guilliermondii var. membranaefaciens 6-(acetylamino)-2-oxohexanoic acid + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate 4.3% of activity compared to L-norleucine Candida guilliermondii var. membranaefaciens oxaloacetate + L-glutamate
-
 ?
L-isoleucine + 2-oxoglutarate
-
 Candida guilliermondii var. membranaefaciens 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-leucine + 2-oxoglutarate
-
 Candida guilliermondii var. membranaefaciens 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-norleucine + 2-oxoglutarate
-
 Candida guilliermondii var. membranaefaciens 2-oxohexanoate + L-glutamate
-
 ?
L-norvaline + 2-oxoglutarate
-
 Candida guilliermondii var. membranaefaciens 2-oxopentanoate + L-glutamate
-
 ?
additional information glycine, L-alanine, L-tyrosine, beta-alanine, epsilon-aminocaproate, delta-aminovalerate, L-arginine, L-lysine or L-ornithine are no substrates Candida guilliermondii var. membranaefaciens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Candida guilliermondii var. membranaefaciens
55
-
-
 Candida guilliermondii var. membranaefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 reaction velocity increases linearly with increasing temperature at pH 7.8, above 55°C activity rapidly decreases, 2-oxoglutarate and to a lesser extent pyridoxal 5'-phosphate are effective protecting agents toward heating Candida guilliermondii var. membranaefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 5 min, about 20% activity remaining activity, with addition of pyridoxal 5'-phosphate 80% remaining activity, with addition of 2-oxoglutarate 100% remaining activity Candida guilliermondii var. membranaefaciens