Application | Comment | Organism |
---|---|---|
analysis | simple, cheap, and sensitive microplate fluorescence assay for 7,8-diaminopelargonic acid aminotransferase activity, allowing linear detection of 7,8-diaminopelargonic acid in the range of 20 nM to 50 microM. The method relies on the direct detection in the enzymatic reaction mixture of the vicinal diamine 7,8-diaminopelargonic acid derivatized with ortho-phthalaldehyde and 2-mercaptoethanol. The assay was validated with the known inhibitor desmethyl-KAPA, i.e. 8-amino-7-oxopelargonic acid, and adapted to microplate for high-throughput screening | Mycobacterium tuberculosis |
analysis | simple, cheap, and sensitive microplate fluorescence assay, allowing linear detection of 7,8-diaminopelargonic acid in the range of 20 nM to 50 microM. The principle of the method is the direct detection in the enzymatic reaction mixture of the vicinal diamine 7,8-diaminopelargonic acid derivatized with ortho-phthalaldehyde and 2-mercaptoethanol. The assay is validated with inhibitor 8-amino-7-oxopelargonic acid and adapted to microplate | Mycobacterium tuberculosis |
analysis | microplate fluorescence assay for 7,8-diaminopelargonic acid aminotransferase that is simple, cheap, and sensitive, allowing linear detection of 7,8-diaminopelargonic acid in the range of 20 nM to 50 microM. The principle of the method is the direct detection in the enzymatic reaction mixture of the vicinal diamine 7,8-diaminopelargonic acid derivatized with ortho-phthalaldehyde and 2-mercaptoethanol | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-amino-7-oxooctanoic acid | - |
Mycobacterium tuberculosis | |
8-amino-7-oxopelargonic acid | - |
Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis | P9WQ81 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WQ81 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 8-amino-7-oxononanoate | - |
Mycobacterium tuberculosis | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | - |
? | |
S-adenosyl-L-methionine + 8-amino-7-oxononanoate | - |
Mycobacterium tuberculosis ATCC 25618 | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7,8-diaminopelargonic acid aminotransferase | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
8-amino-7-oxooctanoic acid | Kic value, pH 9.4, 25°C | Mycobacterium tuberculosis | |
0.015 | - |
8-amino-7-oxooctanoic acid | Kiu value, pH 9.4, 25°C | Mycobacterium tuberculosis |