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Literature summary for 2.6.1.62 extracted from

  • Van Arsdell, S.W.; Perkins, J.B.; Yocum, R.R.; Luan, L.; Howitt, C.L.; Chatterjee, N.P.; Pero, J.G.
    Removing a bottleneck in the Bacillus subtilis biotin pathway: bioA utilizes lysine rather than S-adenosylmethionine as the amino donor in the KAPA-to-DAPA reaction (2005), Biotechnol. Bioeng., 91, 75-83.
    View publication on PubMed
Search for more literature for 2.6.1.62

Application

Application Comment Organism
biotechnology evaluation of biotin overproduction, biotin production by Bacillus subtilis fermentation can be optimized by addition of exogenous lysine or mutational deregulation of lysine in the producing strain Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in strain PY79 Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
8-Amino-7-oxononanoate substrate inhibition above 0.08 mM Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-lysine + 8-amino-7-oxononanoate Bacillus subtilis L-lysine is preferred ? + (7S,8R)-diaminononanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 gene bioA, strains BI282 and BI603, no activity in strain BI9
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate double displacement or ping pong reaction mechanism Bacillus subtilis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 bioindicator assay method, overview Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-lysine + 8-amino-7-oxononanoate L-lysine is preferred Bacillus subtilis ? + (7S,8R)-diaminononanoate
-
 ?
additional information enzyme does not utilize S-adenosyl-L-methionine, Nalpha-acetyl-L-lysine, Nepsilon-acetyl-L-lysine, Nepsilon-methyl-L-lysine, glycine-lysine dipeptide, lysine-glycine dipeptide, and diaminopimelic acid as amino donors Bacillus subtilis ?
-
 ?
S-2-aminoethyl-L-cysteine + 8-amino-7-oxononanoate
-
 Bacillus subtilis ? + (7S,8R)-diaminononanoate
-
 ?

Synonyms

Synonyms Comment Organism
DAPA amino transferase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Bacillus subtilis