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Literature summary for 2.6.1.62 extracted from

  • Mann, S.; Marquet, A.; Ploux, O.
    Inhibition of 7,8-diaminopelargonic acid aminotransferase by amiclenomycin and analogues (2005), Biochem. Soc. Trans., 33, 802-805.
    View publication on PubMed
Search for more literature for 2.6.1.62

Application

Application Comment Organism
pharmacology enzyme is a potential drug target in tuberculosis treatment Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
overexpression as mostly insoluble, N-terminally His6-tagged enzyme in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
8-Amino-7-oxononanoate
-
 Escherichia coli
amiclenomycin and analogues Mycobacterium tuberculosis
cis-amiclenomycin and analogues, overview, irreversible inactivation, kinact. 0.4 min-1, little or no inhibition by the trans-compound, overview Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Escherichia coli
0.0012
-
 8-Amino-7-oxononanoate native enzyme Escherichia coli
0.2
-
 S-adenosyl-L-methionine native enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate Escherichia coli involved in the biosynthesis of biotin S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 ?
S-adenosyl-L-methionine + 8-amino-7-oxononanoate Mycobacterium tuberculosis involved in the biosynthesis of biotin S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate structural basis for reaction mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Mycobacterium tuberculosis S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 ?
S-adenosyl-L-methionine + 8-amino-7-oxononanoate involved in the biosynthesis of biotin Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 ?
S-adenosyl-L-methionine + 8-amino-7-oxononanoate involved in the biosynthesis of biotin Mycobacterium tuberculosis S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 ?
S-adenosyl-L-methionine + 8-amino-7-oxononanoate first half-reaction is reversible, the second one is irreversible Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 ?

Synonyms

Synonyms Comment Organism
7,8-diaminopelargonic acid aminotransferase
-
 Escherichia coli
7,8-diaminopelargonic acid aminotransferase
-
 Mycobacterium tuberculosis
DAPA AT
-
 Escherichia coli
DAPA AT
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 S-adenosyl-L-methionine recombinant enzyme Escherichia coli
0.13
-
 S-adenosyl-L-methionine native enzyme Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Escherichia coli
pyridoxal 5'-phosphate dependent on Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inactivation kinetics Escherichia coli
0.002
-
 amiclenomycin native enzyme Escherichia coli
0.025
-
 8-Amino-7-oxononanoate native enzyme Escherichia coli