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Literature summary for 2.6.1.60 extracted from
- Aromatic-amino acid-glyoxylate aminotransferase from rat liver (1987), Methods Enzymol., 142, 273-279.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | - |
Rattus norvegicus |  |
| Isonicotinic hydrazide | - |
Rattus norvegicus | |
| KCN | - |
Rattus norvegicus | |
| Semicarbazide | strong | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
glyoxylate | cosubstrate L-alanine, 37°C | Rattus norvegicus | |
| 0.07 | - |
pyruvate | cosubstrate L-serine, 37°C | Rattus norvegicus | |
| 1.5 | - |
pyruvate | cosubstrate L-histidine, 37°C | Rattus norvegicus | |
| 2.1 | - |
alanine | cosubstrate L-glyoxylate, 37°C | Rattus norvegicus | |
| 2.6 | - |
phenylalanine | cosubstrate pyruvate, 37°C | Rattus norvegicus | |
| 2.7 | - |
histidine | cosubstrate pyruvate, 37°C | Rattus norvegicus | |
| 5.1 | - |
pyruvate | cosubstrate L-phenylalanine, 37°C | Rattus norvegicus | |
| 16 | - |
serine | cosubstrate pyruvate, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mammalia | 5739 | - |
| mitochondrion | matrix | Rattus norvegicus | 5739 | - |
| additional information | subcellular distribution distribution in different mammalia | Mammalia | - |
- |
| peroxisome | - |
Mammalia | 5777 | - |
| peroxisome | matrix and soluble | Rattus norvegicus | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Rattus norvegicus |
| 80000 | - |
gel filtration | Rattus norvegicus |
| 80000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mammalia | - |
distribution among mammals | - |
| Rattus norvegicus | - |
mitochondrial enzyme inducible by glucagon or cAMP, peroxisomal enzyme inducible by clofibrate | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mammalia | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.9 | - |
substrates histidine + pyruvate, 37°C | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least 6 weeks | Rattus norvegicus |
| 0-5°C, 2 weeks with little or no loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| asparagine + glyoxylate | less efficient amino group donor than L-phenylalanine | Rattus norvegicus | 2-oxosuccinamate + glycine | - |
? | |
| glutamine + glyoxylate | less efficient amino group donor than L-phenylalanine | Rattus norvegicus | 4-carbamoyl-2-oxobutanoate + glycine | - |
? | |
| isoleucine + glyoxylate | poor substrate | Rattus norvegicus | 3-methyl-2-oxopentanoate + glycine | - |
? | |
| L-glutamate + glyoxylate | poor substrate | Rattus norvegicus | 2-oxoglutarate + glycine | - |
? | |
| L-histidine + glyoxylate | less efficient amino group donor than L-phenylalanine | Rattus norvegicus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine | - |
? | |
| L-kynurenine + glyoxylate | - |
Rattus norvegicus | 3-(2-aminophenyl-)2,4-dioxobutanoate + glycine | - |
? | |
| L-leucine + glyoxylate | less efficient amino group donor than L-phenylalanine | Rattus norvegicus | 4-methyl-2-oxopentanoate + glycine | - |
? | |
| L-methionine + glyoxylate | less efficient amino group donor than L-phenylalanine | Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate + glycine | - |
? | |
| L-phenylalanine + 2-oxo-4-methylthiobutyrate | - |
Rattus norvegicus | phenylpyruvate + L-methionine | - |
? | |
| L-phenylalanine + glyoxylate | - |
Rattus norvegicus | phenylpyruvate + glycine | - |
? | |
| L-phenylalanine + hydroxypyruvate | - |
Rattus norvegicus | phenylpyruvate + L-serine | - |
? | |
| L-phenylalanine + pyruvate | - |
Rattus norvegicus | phenylpyruvate + L-alanine | - |
? | |
| L-serine + glyoxylate | less efficient amino group donor than L-phenylalanine | Rattus norvegicus | 3-hydroxy-2-oxopropanoate + glycine | - |
? | |
| tyrosine + glyoxylate | less efficient amino group donor than L-phenylalanine | Rattus norvegicus | 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine | - |
? | |
| valine + glyoxylate | poor substrate | Rattus norvegicus | 3-methyl-2-oxobutanoate + glycine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | requirement | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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