BRENDA - Enzyme Database show
show all sequences of 2.6.1.60

Aromatic-amino acid-glyoxylate aminotransferase from rat liver

Takada, Y.; Noguchi, T.; Methods Enzymol. 142, 273-279 (1987)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
hydroxylamine
-
Rattus norvegicus
Isonicotinic hydrazide
-
Rattus norvegicus
KCN
-
Rattus norvegicus
Semicarbazide
strong
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
glyoxylate
cosubstrate L-alanine, 37°C
Rattus norvegicus
0.07
-
pyruvate
cosubstrate L-serine, 37°C
Rattus norvegicus
1.5
-
pyruvate
cosubstrate L-histidine, 37°C
Rattus norvegicus
2.1
-
alanine
cosubstrate L-glyoxylate, 37°C
Rattus norvegicus
2.6
-
phenylalanine
cosubstrate pyruvate, 37°C
Rattus norvegicus
2.7
-
histidine
cosubstrate pyruvate, 37°C
Rattus norvegicus
5.1
-
pyruvate
cosubstrate L-phenylalanine, 37°C
Rattus norvegicus
16
-
serine
cosubstrate pyruvate, 37°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Mammalia
5739
-
mitochondrion
matrix
Rattus norvegicus
5739
-
additional information
subcellular distribution distribution in different mammalia
Mammalia
-
-
peroxisome
-
Mammalia
5777
-
peroxisome
matrix and soluble
Rattus norvegicus
5777
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Rattus norvegicus
80000
-
gel filtration; sucrose density gradient centrifugation
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mammalia
-
distribution among mammals
-
Rattus norvegicus
-
mitochondrial enzyme inducible by glucagon or cAMP, peroxisomal enzyme inducible by clofibrate
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Mammalia
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
12.9
-
substrates histidine + pyruvate, 37°C
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20°C, at least 6 weeks
Rattus norvegicus
0-5°C, 2 weeks with little or no loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
asparagine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
2-oxosuccinamate + glycine
-
-
-
?
glutamine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
4-carbamoyl-2-oxobutanoate + glycine
-
-
-
?
isoleucine + glyoxylate
poor substrate
637008
Rattus norvegicus
3-methyl-2-oxopentanoate + glycine
-
-
-
?
L-glutamate + glyoxylate
poor substrate
637008
Rattus norvegicus
2-oxoglutarate + glycine
-
-
-
?
L-histidine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
-
-
?
L-kynurenine + glyoxylate
-
637008
Rattus norvegicus
3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
-
-
?
L-leucine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
4-methyl-2-oxopentanoate + glycine
-
-
-
?
L-methionine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
4-methylsulfanyl-2-oxobutanoate + glycine
-
-
-
-
L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
637008
Rattus norvegicus
phenylpyruvate + L-methionine
-
-
-
?
L-phenylalanine + glyoxylate
-
637008
Rattus norvegicus
phenylpyruvate + glycine
-
637008
Rattus norvegicus
?
L-phenylalanine + hydroxypyruvate
-
637008
Rattus norvegicus
phenylpyruvate + L-serine
-
-
-
?
L-phenylalanine + pyruvate
-
637008
Rattus norvegicus
phenylpyruvate + L-alanine
-
-
-
?
L-serine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
3-hydroxy-2-oxopropanoate + glycine
-
-
-
-
tyrosine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
-
-
?
valine + glyoxylate
poor substrate
637008
Rattus norvegicus
3-methyl-2-oxobutanoate + glycine
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydroxylamine
-
Rattus norvegicus
Isonicotinic hydrazide
-
Rattus norvegicus
KCN
-
Rattus norvegicus
Semicarbazide
strong
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
glyoxylate
cosubstrate L-alanine, 37°C
Rattus norvegicus
0.07
-
pyruvate
cosubstrate L-serine, 37°C
Rattus norvegicus
1.5
-
pyruvate
cosubstrate L-histidine, 37°C
Rattus norvegicus
2.1
-
alanine
cosubstrate L-glyoxylate, 37°C
Rattus norvegicus
2.6
-
phenylalanine
cosubstrate pyruvate, 37°C
Rattus norvegicus
2.7
-
histidine
cosubstrate pyruvate, 37°C
Rattus norvegicus
5.1
-
pyruvate
cosubstrate L-phenylalanine, 37°C
Rattus norvegicus
16
-
serine
cosubstrate pyruvate, 37°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Mammalia
5739
-
mitochondrion
matrix
Rattus norvegicus
5739
-
additional information
subcellular distribution distribution in different mammalia
Mammalia
-
-
peroxisome
-
Mammalia
5777
-
peroxisome
matrix and soluble
Rattus norvegicus
5777
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Rattus norvegicus
80000
-
gel filtration; sucrose density gradient centrifugation
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Mammalia
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
12.9
-
substrates histidine + pyruvate, 37°C
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, at least 6 weeks
Rattus norvegicus
0-5°C, 2 weeks with little or no loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
asparagine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
2-oxosuccinamate + glycine
-
-
-
?
glutamine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
4-carbamoyl-2-oxobutanoate + glycine
-
-
-
?
isoleucine + glyoxylate
poor substrate
637008
Rattus norvegicus
3-methyl-2-oxopentanoate + glycine
-
-
-
?
L-glutamate + glyoxylate
poor substrate
637008
Rattus norvegicus
2-oxoglutarate + glycine
-
-
-
?
L-histidine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
-
-
?
L-kynurenine + glyoxylate
-
637008
Rattus norvegicus
3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
-
-
?
L-leucine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
4-methyl-2-oxopentanoate + glycine
-
-
-
?
L-methionine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
4-methylsulfanyl-2-oxobutanoate + glycine
-
-
-
-
L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
637008
Rattus norvegicus
phenylpyruvate + L-methionine
-
-
-
?
L-phenylalanine + glyoxylate
-
637008
Rattus norvegicus
phenylpyruvate + glycine
-
637008
Rattus norvegicus
?
L-phenylalanine + hydroxypyruvate
-
637008
Rattus norvegicus
phenylpyruvate + L-serine
-
-
-
?
L-phenylalanine + pyruvate
-
637008
Rattus norvegicus
phenylpyruvate + L-alanine
-
-
-
?
L-serine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
3-hydroxy-2-oxopropanoate + glycine
-
-
-
-
tyrosine + glyoxylate
less efficient amino group donor than L-phenylalanine
637008
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
-
-
?
valine + glyoxylate
poor substrate
637008
Rattus norvegicus
3-methyl-2-oxobutanoate + glycine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Other publictions for EC 2.6.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
637008
Takada
Aromatic-amino acid-glyoxylate ...
Mammalia, Rattus norvegicus
Methods Enzymol.
142
273-279
1987
-
-
-
-
-
-
4
8
5
-
2
-
-
2
-
-
1
-
-
2
1
2
15
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
8
5
-
2
-
-
-
-
1
-
2
1
2
15
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
636995
Harada
Purification and characterizat ...
Macaca fuscata, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
359
481-488
1978
-
-
-
-
-
-
8
4
2
-
4
1
-
2
-
-
2
-
-
2
2
4
21
2
2
-
-
-
4
-
-
2
-
-
-
-
-
-
2
-
-
-
-
8
-
4
2
-
4
1
-
-
-
2
-
2
2
4
21
2
2
-
-
-
4
-
-
-
-
-
-
-
-
-