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show all sequences of 2.6.1.60

Purification and characterization of aromatic-amino-acid-glyoxylate aminotransferase from monkey and rat liver

Harada, I.; Noguchi, T.; Kido, R.; Hoppe-Seyler's Z. Physiol. Chem. 359, 481-488 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
hydroxylamine
-
Macaca fuscata
hydroxylamine
-
Rattus norvegicus
Isonicotinic hydrazide
-
Macaca fuscata
Isonicotinic hydrazide
-
Rattus norvegicus
KCN
-
Macaca fuscata
KCN
-
Rattus norvegicus
Semicarbazide
strong
Macaca fuscata
Semicarbazide
strong
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
glyoxylate
cosubstrate L-kynurenine, pH 8.0, 37°C
Macaca fuscata
0.5
-
glyoxylate
L-kynurenine, pH 8.0, 37°C
Rattus norvegicus
1.2
-
L-kynurenine
glyoxylate, pH 8.0, 37°C
Rattus norvegicus
1.5
-
L-kynurenine
cosubstrate glyoxylate, pH 8.0, 37°C
Macaca fuscata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Macaca fuscata
5739
-
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Macaca fuscata
40000
-
2 * 40000, SDS-PAGE
Rattus norvegicus
80000
-
gel filtration
Macaca fuscata
80000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an aromatic amino acid + glyoxylate
Rattus norvegicus
-
an aromatic oxo acid + glycine
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Macaca fuscata
-
Blyth monkey
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
partial
Macaca fuscata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Macaca fuscata
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.105
-
pH 7.5, 37°C
Macaca fuscata
0.223
-
pH 7.5, 37°C
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20°C, at least 4 weeks
Macaca fuscata
-20°C, at least 7 weeks
Rattus norvegicus
0-4°C, 2 weeks with little or no loss of activity
Macaca fuscata
0-4°C, 3 weeks with little or no loss of activity respectively
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxytryptophan + glyoxylate
less efficient amino group donor than L-phenylalanine
636995
Rattus norvegicus
3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
-
-
?
5-hydroxytryptophan + glyoxylate
less efficient amino group donor than L-phenylalanine
636995
Macaca fuscata
3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
-
-
?
an aromatic amino acid + glyoxylate
-
636995
Rattus norvegicus
an aromatic oxo acid + glycine
-
-
-
-
L-histidine + glyoxylate
-
636995
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
-
-
?
L-histidine + glyoxylate
-
636995
Macaca fuscata
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
-
-
?
L-kynurenine + glyoxylate
-
636995
Macaca fuscata
3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
-
-
?
L-kynurenine + glyoxylate
poor amino group donor
636995
Rattus norvegicus
3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
-
-
?
L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
636995
Rattus norvegicus
phenylpyruvate + L-methionine
-
-
-
?
L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
636995
Macaca fuscata
phenylpyruvate + L-methionine
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
636995
Rattus norvegicus
phenylpyruvate + L-glutamate
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
636995
Macaca fuscata
phenylpyruvate + L-glutamate
-
-
-
?
L-phenylalanine + glyoxylate
-
636995
Rattus norvegicus
phenylpyruvate + glycine
-
-
-
?
L-phenylalanine + glyoxylate
-
636995
Macaca fuscata
phenylpyruvate + glycine
-
-
-
?
L-phenylalanine + hydroxypyruvate
-
636995
Rattus norvegicus
phenylpyruvate + L-serine
-
-
-
?
L-phenylalanine + hydroxypyruvate
-
636995
Macaca fuscata
phenylpyruvate + L-serine
-
-
-
?
L-phenylalanine + pyruvate
-
636995
Rattus norvegicus
phenylpyruvate + L-alanine
-
-
-
?
L-phenylalanine + pyruvate
-
636995
Macaca fuscata
phenylpyruvate + L-alanine
-
-
-
?
tryptophan + glyoxylate
less efficient amino group donor than L-phenylalanine
636995
Rattus norvegicus
3-indole-2-oxopropanoate + glycine
-
-
-
?
tryptophan + glyoxylate
less efficient amino group donor than L-phenylalanine
636995
Macaca fuscata
3-indole-2-oxopropanoate + glycine
-
-
-
?
tyrosine + glyoxylate
-
636995
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
-
-
?
tyrosine + glyoxylate
-
636995
Macaca fuscata
3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Macaca fuscata
dimer
2 * 40000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Macaca fuscata
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 8
Macaca fuscata
additional information
-
pI: 8
Rattus norvegicus
8
8.5
-
Macaca fuscata
8
8.5
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement
Macaca fuscata
pyridoxal 5'-phosphate
requirement
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement
Macaca fuscata
pyridoxal 5'-phosphate
requirement
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydroxylamine
-
Macaca fuscata
hydroxylamine
-
Rattus norvegicus
Isonicotinic hydrazide
-
Macaca fuscata
Isonicotinic hydrazide
-
Rattus norvegicus
KCN
-
Macaca fuscata
KCN
-
Rattus norvegicus
Semicarbazide
strong
Macaca fuscata
Semicarbazide
strong
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
glyoxylate
cosubstrate L-kynurenine, pH 8.0, 37°C
Macaca fuscata
0.5
-
glyoxylate
L-kynurenine, pH 8.0, 37°C
Rattus norvegicus
1.2
-
L-kynurenine
glyoxylate, pH 8.0, 37°C
Rattus norvegicus
1.5
-
L-kynurenine
cosubstrate glyoxylate, pH 8.0, 37°C
Macaca fuscata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Macaca fuscata
5739
-
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Macaca fuscata
40000
-
2 * 40000, SDS-PAGE
Rattus norvegicus
80000
-
gel filtration
Macaca fuscata
80000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
an aromatic amino acid + glyoxylate
Rattus norvegicus
-
an aromatic oxo acid + glycine
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
partial
Macaca fuscata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Macaca fuscata
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.105
-
pH 7.5, 37°C
Macaca fuscata
0.223
-
pH 7.5, 37°C
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, at least 4 weeks
Macaca fuscata
-20°C, at least 7 weeks
Rattus norvegicus
0-4°C, 2 weeks with little or no loss of activity
Macaca fuscata
0-4°C, 3 weeks with little or no loss of activity respectively
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxytryptophan + glyoxylate
less efficient amino group donor than L-phenylalanine
636995
Rattus norvegicus
3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
-
-
?
5-hydroxytryptophan + glyoxylate
less efficient amino group donor than L-phenylalanine
636995
Macaca fuscata
3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
-
-
?
an aromatic amino acid + glyoxylate
-
636995
Rattus norvegicus
an aromatic oxo acid + glycine
-
-
-
-
L-histidine + glyoxylate
-
636995
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
-
-
?
L-histidine + glyoxylate
-
636995
Macaca fuscata
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
-
-
?
L-kynurenine + glyoxylate
-
636995
Macaca fuscata
3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
-
-
?
L-kynurenine + glyoxylate
poor amino group donor
636995
Rattus norvegicus
3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
-
-
?
L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
636995
Rattus norvegicus
phenylpyruvate + L-methionine
-
-
-
?
L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
636995
Macaca fuscata
phenylpyruvate + L-methionine
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
636995
Rattus norvegicus
phenylpyruvate + L-glutamate
-
-
-
?
L-phenylalanine + 2-oxoglutarate
-
636995
Macaca fuscata
phenylpyruvate + L-glutamate
-
-
-
?
L-phenylalanine + glyoxylate
-
636995
Rattus norvegicus
phenylpyruvate + glycine
-
-
-
?
L-phenylalanine + glyoxylate
-
636995
Macaca fuscata
phenylpyruvate + glycine
-
-
-
?
L-phenylalanine + hydroxypyruvate
-
636995
Rattus norvegicus
phenylpyruvate + L-serine
-
-
-
?
L-phenylalanine + hydroxypyruvate
-
636995
Macaca fuscata
phenylpyruvate + L-serine
-
-
-
?
L-phenylalanine + pyruvate
-
636995
Rattus norvegicus
phenylpyruvate + L-alanine
-
-
-
?
L-phenylalanine + pyruvate
-
636995
Macaca fuscata
phenylpyruvate + L-alanine
-
-
-
?
tryptophan + glyoxylate
less efficient amino group donor than L-phenylalanine
636995
Rattus norvegicus
3-indole-2-oxopropanoate + glycine
-
-
-
?
tryptophan + glyoxylate
less efficient amino group donor than L-phenylalanine
636995
Macaca fuscata
3-indole-2-oxopropanoate + glycine
-
-
-
?
tyrosine + glyoxylate
-
636995
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
-
-
?
tyrosine + glyoxylate
-
636995
Macaca fuscata
3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Macaca fuscata
dimer
2 * 40000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Macaca fuscata
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 8
Macaca fuscata
additional information
-
pI: 8
Rattus norvegicus
8
8.5
-
Macaca fuscata
8
8.5
-
Rattus norvegicus
Other publictions for EC 2.6.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
637008
Takada
Aromatic-amino acid-glyoxylate ...
Mammalia, Rattus norvegicus
Methods Enzymol.
142
273-279
1987
-
-
-
-
-
-
4
8
5
-
2
-
-
2
-
-
1
-
-
2
1
2
15
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
8
5
-
2
-
-
-
-
1
-
2
1
2
15
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
636995
Harada
Purification and characterizat ...
Macaca fuscata, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
359
481-488
1978
-
-
-
-
-
-
8
4
2
-
4
1
-
2
-
-
2
-
-
2
2
4
21
2
2
-
-
-
4
-
-
2
-
-
-
-
-
-
2
-
-
-
-
8
-
4
2
-
4
1
-
-
-
2
-
2
2
4
21
2
2
-
-
-
4
-
-
-
-
-
-
-
-
-