BRENDA - Enzyme Database
show all sequences of 2.6.1.6

Transaminases of branched chain amino acids. IV. Purification and properties of two enzymes from rat liver

Aki, K.; Ogawa, K.; Ichihara, A.; Biochim. Biophys. Acta 159, 276-284 (1968)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
slight inhibition
Rattus norvegicus
p-chloromercuribenzoate
1.0 mM, complete inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
2-oxoglutarate
pH 8.2, 37°C
Rattus norvegicus
25
-
leucine
pH 8.2, 37°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
predominantly found in mitochondrion
Rattus norvegicus
5737
-
microsome
-
Rattus norvegicus
-
-
mitochondrion
-
Rattus norvegicus
5739
-
nucleus
-
Rattus norvegicus
5634
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-leucine + 2-oxoglutarate
Rattus norvegicus
leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly
4-methyl-2-oxopentanoate + L-glutamate
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.033
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-leucine + 2-oxoglutarate
-
639996
Rattus norvegicus
4-methyl-2-oxopentanoate + L-glutamate
-
639996
Rattus norvegicus
r
L-leucine + 2-oxoglutarate
leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly
639996
Rattus norvegicus
4-methyl-2-oxopentanoate + L-glutamate
-
639996
Rattus norvegicus
?
L-leucine + oxaloacetate
-
639996
Rattus norvegicus
4-methyl-2-oxopentanoate + L-aspartate
-
-
-
?
additional information
specific for leucine, isoleucine or valine, no other amino acid would serve as an amino donor, pyruvate is no amino acid acceptor
639996
Rattus norvegicus
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
Km 0.004 mM
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
Km 0.004 mM
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
slight inhibition
Rattus norvegicus
p-chloromercuribenzoate
1.0 mM, complete inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
2-oxoglutarate
pH 8.2, 37°C
Rattus norvegicus
25
-
leucine
pH 8.2, 37°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
predominantly found in mitochondrion
Rattus norvegicus
5737
-
microsome
-
Rattus norvegicus
-
-
mitochondrion
-
Rattus norvegicus
5739
-
nucleus
-
Rattus norvegicus
5634
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-leucine + 2-oxoglutarate
Rattus norvegicus
leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly
4-methyl-2-oxopentanoate + L-glutamate
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.033
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-leucine + 2-oxoglutarate
-
639996
Rattus norvegicus
4-methyl-2-oxopentanoate + L-glutamate
-
639996
Rattus norvegicus
r
L-leucine + 2-oxoglutarate
leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly
639996
Rattus norvegicus
4-methyl-2-oxopentanoate + L-glutamate
-
639996
Rattus norvegicus
?
L-leucine + oxaloacetate
-
639996
Rattus norvegicus
4-methyl-2-oxopentanoate + L-aspartate
-
-
-
?
additional information
specific for leucine, isoleucine or valine, no other amino acid would serve as an amino donor, pyruvate is no amino acid acceptor
639996
Rattus norvegicus
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Rattus norvegicus
Other publictions for EC 2.6.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
640169
Singh
-
Purification and characterizat ...
Amaranthus dubius
Biochem. Physiol. Pflanz.
187
337-345
1991
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640014
Korpela
Purification of branched-chain ...
Rattus norvegicus
Methods Enzymol.
166
269-274
1988
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640167
Pathre
-
Purification and properties of ...
Glycine max
Phytochemistry
26
2913-2917
1987
-
-
-
-
-
-
11
4
-
-
2
1
-
1
-
-
1
-
-
1
2
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
4
-
-
2
1
-
-
-
1
-
1
2
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640166
Ikeda
Transaminase of branched chain ...
Rattus norvegicus
Biochim. Biophys. Acta
445
622-631
1976
-
-
-
-
-
-
-
-
1
-
1
2
-
1
-
-
1
-
-
1
-
-
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
1
-
1
-
-
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
639996
Aki
Transaminases of branched chai ...
Rattus norvegicus
Biochim. Biophys. Acta
159
276-284
1968
-
-
-
-
-
-
2
2
4
-
-
1
-
1
-
-
1
-
-
1
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
4
-
-
1
-
-
-
1
-
1
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-