BRENDA - Enzyme Database
show all sequences of 2.6.1.59

Thymidine diphosphate 4-acetamido-4,6-dideoxyhexoses. IV. Purification and properties of thymidine diphosphate 4-keto-6-deoxy-D-glucose transaminase from Pasteurella pseudotuberculosis

Ohashi, H.; Matsuhashi, M.; Matsuhashi, S.; J. Biol. Chem. 246, 2325-2330 (1971)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Maleate
stabilizing effect
Yersinia pseudotuberculosis
General Stability
General Stability
Organism
crude extract and partially purified enzyme are unstable, necessity of addition of pyridoxal phosphate during purification
Yersinia pseudotuberculosis
maleate enhances stabilizing effect of pyridoxal phosphate
Yersinia pseudotuberculosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
L-cysteine
strong
Yersinia pseudotuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
TDP-4-amino-4,6-dideoxy-D-galactose
pH 7.0, 38°C
Yersinia pseudotuberculosis
0.22
-
2-oxoglutarate
pH 7.0, 38°C
Yersinia pseudotuberculosis
1
-
TDP-4-keto-6-deoxy-D-glucose
pH 7.0, 38°C
Yersinia pseudotuberculosis
7.9
-
L-glutamate
pH 7.0, 38°C
Yersinia pseudotuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
Yersinia pseudotuberculosis
-
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
-
?
dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
Yersinia pseudotuberculosis VO
-
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Yersinia pseudotuberculosis
-
type VO
-
Yersinia pseudotuberculosis VO
-
type VO
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Yersinia pseudotuberculosis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.01
-
pH 7.0, 38°C
Yersinia pseudotuberculosis
Storage Stability
Storage Stability
Organism
0°C or -15°C, purified enzyme, stable for several weeks
Yersinia pseudotuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
636976
Yersinia pseudotuberculosis
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
-
-
?
dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
636976
Yersinia pseudotuberculosis VO
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
-
-
?
additional information
little activity with L-glutamine or L-alanine
636976
Yersinia pseudotuberculosis
?
-
-
-
?
additional information
little activity with L-glutamine or L-alanine
636976
Yersinia pseudotuberculosis VO
?
-
-
-
?
TDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
636976
Yersinia pseudotuberculosis
TDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
636976
Yersinia pseudotuberculosis
r
TDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
636976
Yersinia pseudotuberculosis VO
TDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
636976
Yersinia pseudotuberculosis VO
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
assay at
Yersinia pseudotuberculosis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
-
10 min: 7% remaining activity, 72% remaining activity with addition of pyridoxal 5'-phosphate, 86% remaining activity with addition of pyridoxal 5'-phosphate and potassium maleate
Yersinia pseudotuberculosis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Yersinia pseudotuberculosis
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
absolute requirement, a pyridoxal phosphate protein
Yersinia pseudotuberculosis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Maleate
stabilizing effect
Yersinia pseudotuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
absolute requirement, a pyridoxal phosphate protein
Yersinia pseudotuberculosis
General Stability (protein specific)
General Stability
Organism
crude extract and partially purified enzyme are unstable, necessity of addition of pyridoxal phosphate during purification
Yersinia pseudotuberculosis
maleate enhances stabilizing effect of pyridoxal phosphate
Yersinia pseudotuberculosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-cysteine
strong
Yersinia pseudotuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
TDP-4-amino-4,6-dideoxy-D-galactose
pH 7.0, 38°C
Yersinia pseudotuberculosis
0.22
-
2-oxoglutarate
pH 7.0, 38°C
Yersinia pseudotuberculosis
1
-
TDP-4-keto-6-deoxy-D-glucose
pH 7.0, 38°C
Yersinia pseudotuberculosis
7.9
-
L-glutamate
pH 7.0, 38°C
Yersinia pseudotuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
Yersinia pseudotuberculosis
-
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
-
?
dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
Yersinia pseudotuberculosis VO
-
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Yersinia pseudotuberculosis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.01
-
pH 7.0, 38°C
Yersinia pseudotuberculosis
Storage Stability (protein specific)
Storage Stability
Organism
0°C or -15°C, purified enzyme, stable for several weeks
Yersinia pseudotuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
636976
Yersinia pseudotuberculosis
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
-
-
?
dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
636976
Yersinia pseudotuberculosis VO
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
-
-
?
additional information
little activity with L-glutamine or L-alanine
636976
Yersinia pseudotuberculosis
?
-
-
-
?
additional information
little activity with L-glutamine or L-alanine
636976
Yersinia pseudotuberculosis VO
?
-
-
-
?
TDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
636976
Yersinia pseudotuberculosis
TDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
636976
Yersinia pseudotuberculosis
r
TDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
636976
Yersinia pseudotuberculosis VO
TDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
636976
Yersinia pseudotuberculosis VO
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
assay at
Yersinia pseudotuberculosis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
-
10 min: 7% remaining activity, 72% remaining activity with addition of pyridoxal 5'-phosphate, 86% remaining activity with addition of pyridoxal 5'-phosphate and potassium maleate
Yersinia pseudotuberculosis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Yersinia pseudotuberculosis
Other publictions for EC 2.6.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
658427
Hwang
Characterization and investiga ...
Escherichia coli
Chem. Biol.
11
915-925
2004
-
-
1
-
-
-
3
1
-
-
2
-
-
1
-
-
1
-
-
-
-
-
3
1
1
1
1
-
1
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
3
-
1
-
-
2
-
-
-
-
1
-
-
-
-
3
1
1
1
-
1
1
1
-
-
-
-
-
-
1
1
636976
Ohashi
Thymidine diphosphate 4-acetam ...
Yersinia pseudotuberculosis, Yersinia pseudotuberculosis VO
J. Biol. Chem.
246
2325-2330
1971
1
-
-
-
-
2
1
4
-
-
-
2
-
6
-
-
1
-
-
-
1
1
6
-
-
1
-
1
-
1
-
-
1
-
-
-
1
-
-
1
-
-
2
-
1
-
4
-
-
-
2
-
-
-
1
-
-
1
1
6
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-