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Literature summary for 2.6.1.57 extracted from

  • Andreessen, C.; Gerlt, V.; Steinbuechel, A.
    Conversion of cysteine to 3-mercaptopyruvic acid by bacterial aminotransferases (2017), Enzyme Microb. Technol., 99, 38-48 .
    View publication on PubMed
Search for more literature for 2.6.1.57

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cupriavidus necator
expressed in Escherichia coli BL21(DE3) cells Shimwellia blattae
expressed in Escherichia coli BL21(DE3) cells Paracoccus denitrificans
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.079
-
 L-aspartate at pH 7.4 and 30°C Paracoccus denitrificans
12.22
-
 L-cysteine at pH 7.4 and 30°C Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Cupriavidus necator
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Shimwellia blattae
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Paracoccus denitrificans
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Escherichia coli
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Escherichia coli MG1655
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Shimwellia blattae ATCC 33430
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Paracoccus denitrificans Pd1222
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Escherichia coli P04693
-
-
Escherichia coli MG1655 P04693
-
-
Paracoccus denitrificans P95468
-
-
Paracoccus denitrificans Pd1222 P95468
-
-
Shimwellia blattae
-
-
-
Shimwellia blattae ATCC 33430
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His SpinTrap column chromatography Cupriavidus necator
His SpinTrap column chromatography Shimwellia blattae
His SpinTrap column chromatography Paracoccus denitrificans
His SpinTrap column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Cupriavidus necator oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Shimwellia blattae oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Paracoccus denitrificans oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Escherichia coli oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Escherichia coli MG1655 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Shimwellia blattae ATCC 33430 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Paracoccus denitrificans Pd1222 oxaloacetate + L-glutamate
-
 ?
L-cysteine + 2-oxoglutarate
-
 Cupriavidus necator 3-mercaptopyruvate + L-glutamate
-
 ?
L-cysteine + 2-oxoglutarate
-
 Shimwellia blattae 3-mercaptopyruvate + L-glutamate
-
 ?
L-cysteine + 2-oxoglutarate
-
 Paracoccus denitrificans 3-mercaptopyruvate + L-glutamate
-
 ?
L-cysteine + 2-oxoglutarate
-
 Escherichia coli 3-mercaptopyruvate + L-glutamate
-
 ?
L-cysteine + 2-oxoglutarate
-
 Escherichia coli MG1655 3-mercaptopyruvate + L-glutamate
-
 ?
L-cysteine + 2-oxoglutarate
-
 Shimwellia blattae ATCC 33430 3-mercaptopyruvate + L-glutamate
-
 ?
L-cysteine + 2-oxoglutarate
-
 Paracoccus denitrificans Pd1222 3-mercaptopyruvate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Cupriavidus necator
? x * 40000, SDS-PAGE Shimwellia blattae
? x * 40000, SDS-PAGE Paracoccus denitrificans
? x * 40000, SDS-PAGE Escherichia coli
? x * 43600, calculated from amino acid sequence Cupriavidus necator
? x * 43100, calculated from amino acid sequence Shimwellia blattae
? x * 42700, calculated from amino acid sequence Paracoccus denitrificans
? x * 43500, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
ArAT
-
 Cupriavidus necator
ArAT
-
 Shimwellia blattae
ArAT
-
 Paracoccus denitrificans
ArAT
-
 Escherichia coli
aromatic amino acid aminotransferase
-
 Cupriavidus necator
aromatic amino acid aminotransferase
-
 Shimwellia blattae
aromatic amino acid aminotransferase
-
 Paracoccus denitrificans
aromatic amino acid aminotransferase
-
 Escherichia coli
TyrB
-
 Cupriavidus necator
TyrB
-
 Shimwellia blattae
TyrB
-
 Paracoccus denitrificans
TyrB
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Paracoccus denitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.89
-
 L-cysteine at pH 7.4 and 30°C Paracoccus denitrificans
16.9
-
 L-aspartate at pH 7.4 and 30°C Paracoccus denitrificans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Cupriavidus necator
pyridoxal 5'-phosphate
-
 Shimwellia blattae
pyridoxal 5'-phosphate
-
 Paracoccus denitrificans
pyridoxal 5'-phosphate
-
 Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Shimwellia blattae calculated from amino acid sequence
-
 5.4
Paracoccus denitrificans calculated from amino acid sequence
-
 5.5
Cupriavidus necator calculated from amino acid sequence
-
 6.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4
-
 L-cysteine at pH 7.4 and 30°C Paracoccus denitrificans
214
-
 L-aspartate at pH 7.4 and 30°C Paracoccus denitrificans