Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
3-(4-hydroxyphenyl)pyruvate | pH 8.0, 25°C, cosubstrate aspartate | Paracoccus denitrificans | |
0.083 | - |
phenylpyruvate | pH 8.0, 25°C, cosubstrate aspartate | Paracoccus denitrificans | |
0.14 | - |
2-oxoglutarate | pH 8.0, 25°C, cosubstrate tryptophan | Paracoccus denitrificans | |
0.59 | - |
2-oxoglutarate | pH 8.0, 25°C, cosubstrate aspartate | Paracoccus denitrificans | |
1.4 | - |
L-tryptophan | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Paracoccus denitrificans | |
1.8 | - |
L-aspartate | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Paracoccus denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42653 | - |
2 * 42653, mass spectrometry | Paracoccus denitrificans |
42731 | - |
2 * 42731, deduced from nucleotide sequence | Paracoccus denitrificans |
83000 | - |
gel filtration | Paracoccus denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | P95468 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, phenyl-Toyopearl, hydroxyapatite, Sephacryl S-200, recombinant enzyme | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aspartate + 3-phenylpyruvate | - |
Paracoccus denitrificans | oxaloacetate + L-phenylalanine | - |
r | |
L-aspartate + 2-oxoglutarate | - |
Paracoccus denitrificans | oxaloacetate + L-glutamate | - |
r | |
L-aspartate + 3-(4-hydroxyphenyl)pyruvate | - |
Paracoccus denitrificans | L-tyrosine + oxaloacetate | - |
r | |
L-phenylalanine + 2-oxoglutarate | enzyme also reacts with 2-aminooctanoate and 2-aminoheptanoate | Paracoccus denitrificans | phenylpyruvate + L-glutamate | - |
r | |
L-tryptophan + 2-oxoglutarate | - |
Paracoccus denitrificans | 3-indole-2-oxopropanoate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | - |
Paracoccus denitrificans | p-hydroxyphenylpyruvate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42731, deduced from nucleotide sequence | Paracoccus denitrificans |
dimer | 2 * 42653, mass spectrometry | Paracoccus denitrificans |
Synonyms | Comment | Organism |
---|---|---|
pdArAT | - |
Paracoccus denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 1 pyridoxal 5'-phosphate per subunit | Paracoccus denitrificans |