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Literature summary for 2.6.1.57 extracted from

  • Oue, S.; Okamoto, A.; Nakai, Y.; Nakahira, M.; Shibatani, T.; Hayashi, H.; Kagamiyama, H.
    Paracoccus denitrificans aromatic amino acid aminotransferase: a model enzyme for the study of dual substrate recognition mechanism (1997), J. Biochem., 121, 161-171.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
3-(4-hydroxyphenyl)pyruvate pH 8.0, 25°C, cosubstrate aspartate Paracoccus denitrificans
0.083
-
phenylpyruvate pH 8.0, 25°C, cosubstrate aspartate Paracoccus denitrificans
0.14
-
2-oxoglutarate pH 8.0, 25°C, cosubstrate tryptophan Paracoccus denitrificans
0.59
-
2-oxoglutarate pH 8.0, 25°C, cosubstrate aspartate Paracoccus denitrificans
1.4
-
L-tryptophan pH 8.0, 25°C, cosubstrate 2-oxoglutarate Paracoccus denitrificans
1.8
-
L-aspartate pH 8.0, 25°C, cosubstrate 2-oxoglutarate Paracoccus denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42653
-
2 * 42653, mass spectrometry Paracoccus denitrificans
42731
-
2 * 42731, deduced from nucleotide sequence Paracoccus denitrificans
83000
-
gel filtration Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans P95468
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, phenyl-Toyopearl, hydroxyapatite, Sephacryl S-200, recombinant enzyme Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aspartate + 3-phenylpyruvate
-
Paracoccus denitrificans oxaloacetate + L-phenylalanine
-
r
L-aspartate + 2-oxoglutarate
-
Paracoccus denitrificans oxaloacetate + L-glutamate
-
r
L-aspartate + 3-(4-hydroxyphenyl)pyruvate
-
Paracoccus denitrificans L-tyrosine + oxaloacetate
-
r
L-phenylalanine + 2-oxoglutarate enzyme also reacts with 2-aminooctanoate and 2-aminoheptanoate Paracoccus denitrificans phenylpyruvate + L-glutamate
-
r
L-tryptophan + 2-oxoglutarate
-
Paracoccus denitrificans 3-indole-2-oxopropanoate + L-glutamate
-
r
L-tyrosine + 2-oxoglutarate
-
Paracoccus denitrificans p-hydroxyphenylpyruvate + L-glutamate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 42731, deduced from nucleotide sequence Paracoccus denitrificans
dimer 2 * 42653, mass spectrometry Paracoccus denitrificans

Synonyms

Synonyms Comment Organism
pdArAT
-
Paracoccus denitrificans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 1 pyridoxal 5'-phosphate per subunit Paracoccus denitrificans