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Literature summary for 2.6.1.57 extracted from

  • Weigent, D.A.; Nester, E.W.
    Purification and properties of two aromatic aminotransferases in Bacillus subtilis (1976), J. Biol. Chem., 251, 6974-6980.
    View publication on PubMed
Search for more literature for 2.6.1.57

General Stability

General Stability Organism
2-oxoglutarate or pyridoxal 5'-phosphate stabilizes against heat inactivation Bacillus subtilis
stable to freezing and thawing Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 pyridoxal 5'-phosphate pH 7.5, 37°C Bacillus subtilis
2.7
-
 L-tyrosine pH 7.5, 37°C Bacillus subtilis
9.8
-
 L-phenylalanine pH 7.5, 37°C Bacillus subtilis
38
-
 2-oxoglutarate pH 7.5, 37°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63500
-
 gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
55°C, ammonium sulfate, DE52, phenylalanine-Sepharose, Sephadex G-150 Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
an aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate ping pong mechanism Bacillus subtilis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
95
-
-
 Bacillus subtilis
950
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
0°C, 1 month, 15% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + 2-oxoglutarate
-
 Bacillus subtilis phenylpyruvate + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Bacillus subtilis 3-indole-2-oxopropanoate + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Bacillus subtilis 4-hydroxyphenylpyruvate + L-glutamate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 2-oxoglutarate or pyridoxal 5'-phosphate stabilize against heat inactivation Bacillus subtilis
55
-
 10 min, 90% loss of activity without stabilizing agent, 10% loss of activity in the presence of 0.6 mM pyridoxal 5'-phosphate and 1 mM 2-oxoglutarate Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km: 0.025 mM Bacillus subtilis