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Literature summary for 2.6.1.57 extracted from

  • Kanda, M.; Hori, K.; Kurotsu, T.; Miura, S.; Saito, Y.
    Purification and properties of the aromatic amino acid aminotransferase from gramicidin S-producing Bacillus brevis (1987), J. Biochem., 101, 871-878.
    View publication on PubMed
Search for more literature for 2.6.1.57

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 L-tyrosine pH 8.0 Brevibacillus brevis
0.15
-
 2-oxoglutarate pH 8.0 Brevibacillus brevis
0.17
-
 L-phenylalanine pH 8.0 Brevibacillus brevis
0.21
-
 L-tryptophan pH 8.0 Brevibacillus brevis
3.7
-
 L-aspartate pH 8.0 Brevibacillus brevis
5.1
-
 oxaloacetate pH 8.0 Brevibacillus brevis
7.1
-
 L-leucine pH 8.0 Brevibacillus brevis
8.8
-
 pyruvate pH 8.0 Brevibacillus brevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Brevibacillus brevis
71000
-
 gel filtration Brevibacillus brevis

Organism

Organism UniProt Comment Textmining
Brevibacillus brevis
-
 gramicidin S-producing
-

Purification (Commentary)

Purification (Comment) Organism
gramicidin S-producing strain, ammonium sulfate, heat treatment, DEAE-Sephadex, hydroxylapatite, pyridoxamine 5'-phosphate-Sepharose, Ultrogel Aca-34 Brevibacillus brevis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
353
-
-
 Brevibacillus brevis

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, 10 mM DTT, 0.1 mM pyridoxal phosphate, at least 3 months, no loss of activity Brevibacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aspartate + 2-oxoglutarate 3.1% of the activity with phenylalanine Brevibacillus brevis oxaloacetate + L-glutamate
-
 ?
L-histidine + 2-oxoglutarate 27.8% of activity with phenylalanine Brevibacillus brevis 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
 ?
L-leucine + 2-oxoglutarate
-
 Brevibacillus brevis 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-methionine + 2-oxoglutarate 0.6% of activity with phenylalanine Brevibacillus brevis 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate
-
 Brevibacillus brevis phenylpyruvate + L-glutamate
-
 ?
L-phenylalanine + oxaloacetate 3.5% of activity with 2-oxoglutarate Brevibacillus brevis phenylpyruvate + L-aspartate
-
 ?
L-phenylalanine + pyruvate 2.1% of the activity with 2-oxoglutarate Brevibacillus brevis phenylpyruvate + L-alanine
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Brevibacillus brevis 3-indole-2-oxopropanoate + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Brevibacillus brevis p-hydroxyphenylpyruvate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Brevibacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Brevibacillus brevis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate one mol of pyridoxal phosphate per subunit Brevibacillus brevis