Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-aminobutyrate | stimulation, 177% of non-stimulated activity | Clostridium butyricum | |
D-asparagine | stimulation, 112% of non-stimulated activity | Clostridium butyricum | |
D-cysteine | stimulation, 135% of non-stimulated activity | Clostridium butyricum | |
D-methionine | stimulation, 127% of non-stimulated activity | Clostridium butyricum | |
DL-Ornithine | stimulation, 112% of non-stimulated activity | Clostridium butyricum | |
glycine | stimulation, 137% of non-stimulated activity | Clostridium butyricum | |
L-asparagine | stimulation, 112% of non-stimulated activity | Clostridium butyricum | |
L-glutamate | stimulation, 166% of non-stimulated activity | Clostridium butyricum | |
L-lysine | stimulation, 105% of non-stimulated activity | Clostridium butyricum | |
L-methionine | stimulation, 114% of non-stimulated activity | Clostridium butyricum | |
L-ornithine | stimulation, 112% of non-stimulated activity | Clostridium butyricum | |
additional information | stimulating effect of amino compounds may be caused by disappearance of the inhibitory product of the reaction, pyridoxal 5'-phosphate, forming the corresponding Schiff base | Clostridium butyricum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glutamate | slight inhibition | Clostridium butyricum | |
diisopropyl phosphofluoride | 0.3 mM, complete inhibition | Clostridium butyricum | |
L-cysteine | about 35% inhibition | Clostridium butyricum | |
additional information | not inhibited by sulfhydryl compounds, e.g. PCMB, phenylmercuric acetate and mercuric chloride | Clostridium butyricum | |
pyridoxal 5'-phosphate | product inhibition | Clostridium butyricum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
pyridoxamine 5'-phosphate | pH 8.0, 37°C | Clostridium butyricum | |
0.01 | - |
pyridoxal 5'-phosphate | pH 8.0, 37°C | Clostridium butyricum | |
0.015 | - |
2-oxoglutarate | cosubstrate pyridoxamine 5'-phosphate, pH 8.0, 37°C | Clostridium butyricum | |
9 | - |
D-glutamate | pH 8.0, 37°C | Clostridium butyricum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal ions do not accelerate the reaction | Clostridium butyricum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium butyricum | - |
IFO 3353 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, protamine treatment, DEAE-cellulose, hydroxylapatite, DEAE-Sephadex, Sephadex G-200, partial purification | Clostridium butyricum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.281 | - |
- |
Clostridium butyricum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + 2-oxoglutarate | about 8% of activity compared to pyridoxamine 5'-phosphate | Clostridium butyricum | 4-oxobutanoate + D-glutamate | - |
? | |
pyridoxamine + 2-oxoglutarate | about 25% of activity compared to pyridoxamine 5'-phosphate | Clostridium butyricum | pyridoxal + D-glutamate | - |
? | |
pyridoxamine 5'-phosphate + 2-oxoglutarate | - |
Clostridium butyricum | pyridoxal 5'-phosphate + D-glutamate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium butyricum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Clostridium butyricum |