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Literature summary for 2.6.1.52 extracted from
- Biochemical and functional characterization of phosphoserine aminotransferase from Entamoeba histolytica, which possesses both phosphorylated and non-phosphorylated serine metabolic pathways (2006), Mol. Biochem. Parasitol., 145, 71-83.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
| expression in Escherichia coli | Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| O-acetylserine | 10 mM, 50% inhibition | Entamoeba histolytica |  |
| o-phospho-D-serine | 10 mM, 70% inhibition | Entamoeba histolytica | |
| O-phospho-L-threonine | 10 mM, 65% inhibition | Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0368 | - |
O-phospho-L-serine | pH 8.5, 25°C | Entamoeba histolytica | |
| 0.043 | - |
2-oxoglutarate | pH 8.5, 25°C | Entamoeba histolytica | |
| 0.058 | - |
3-phosphohydroxypyruvate | pH 8.5, 25°C | Entamoeba histolytica | |
| 0.0651 | - |
2-oxoglutarate | pH 8.5, 25°C | Arabidopsis thaliana | |
| 0.225 | - |
O-phospho-L-serine | pH 8.5, 25°C | Arabidopsis thaliana | |
| 0.788 | - |
L-glutamate | pH 8.5, 25°C | Entamoeba histolytica | |
| 0.797 | - |
3-phosphohydroxypyruvate | pH 8.5, 25°C | Arabidopsis thaliana | |
| 5.05 | - |
L-glutamate | pH 8.5, 25°C | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Entamoeba histolytica | Q60I38 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
- |
Entamoeba histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.47 | - |
substrate: O-phospho-L-serine, pH 8.5, 25°C | Arabidopsis thaliana |
| 0.66 | - |
substrate: 2-oxoglutarate, pH 8.5, 25°C | Entamoeba histolytica |
| 0.67 | - |
substrate: O-phospho-L-serine, pH 8.5, 25°C | Entamoeba histolytica |
| 0.74 | - |
substrate: 2-oxoglutarate, pH 8.5, 25°C | Arabidopsis thaliana |
| 5.82 | - |
substrate: L-glutamate, pH 8.5, 25°C | Entamoeba histolytica |
| 6.14 | - |
substrate: 3-phosphohydroxypyruvate, pH 8.5, 25°C | Entamoeba histolytica |
| 9.65 | - |
substrate: 3-phosphohydroxypyruvate, pH 8.5, 25°C | Arabidopsis thaliana |
| 21.22 | - |
substrate: L-glutamate, pH 8.5, 25°C | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + 3-phosphohydroxypyruvate | - |
Arabidopsis thaliana | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
| L-glutamate + 3-phosphohydroxypyruvate | - |
Entamoeba histolytica | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtPSAT | - |
Arabidopsis thaliana |
| EhPSAT | - |
Entamoeba histolytica |
| PSAT | - |
Arabidopsis thaliana |
| PSAT | - |
Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | formation of 3-phosphohydroxypyruvate | Entamoeba histolytica |
| 8 | 9 | formation of O-phospho-L-serine | Entamoeba histolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 11 | about 75% of maximal activit at pH 5.5 and pH 11.0, formation of 3-phosphohydroxypyruvate | Entamoeba histolytica |
| 7 | 10 | about 50% of maximal activity at pH 7.0 and at pH 10.0, formation of O-phospho-L-serine | Entamoeba histolytica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Entamoeba histolytica | His-tagged recombinant enzyme | - |
8.43 |
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