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Literature summary for 2.6.1.5 extracted from

  • Ru, M.; Wang, K.; Bai, Z.; Peng, L.; He, S.; Wang, Y.; Liang, Z.
    A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L (2017), Sci. Rep., 7, 4892 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in tobacco protoplasts, Escherichia coli Rosetta (DE3) cells and Escherichia coli tyrosine auxotrophic mutant DL39 Prunella vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 L-tyrosine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.48
-
 4-hydroxyphenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.84
-
 phenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
1.82
-
 oxaloacetate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
3.9
-
 phenylpyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
4.72
-
 oxaloacetate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
8.14
-
 L-tryptophan with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
10.2
-
 L-phenylalanine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
10.8
-
 2-oxoglutarate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
52.06
-
 pyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Prunella vulgaris 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + 2-oxoglutarate Prunella vulgaris
-
 4-hydroxyphenylpyruvate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Prunella vulgaris
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Prunella vulgaris
-
root highest expression Prunella vulgaris
-
spike
-
 Prunella vulgaris
-
stem
-
 Prunella vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + 4-hydroxyphenylpyruvate
-
 Prunella vulgaris 2-oxoglutarate + L-tyrosine
-
 r
L-glutamate + oxaloacetate
-
 Prunella vulgaris ?
-
 r
L-glutamate + phenylpyruvate
-
 Prunella vulgaris 2-oxoglutarate + L-phenylalanine
-
 r
L-phenylalanine + 2-oxoglutarate
-
 Prunella vulgaris phenylpyruvate + L-glutamate
-
 r
L-tryptophan + 2-oxoglutarate
-
 Prunella vulgaris 3-(1H-indol-3-yl)-2-oxopropanoate + L-glutamate
-
 r
L-tyrosine + 2-oxoglutarate
-
 Prunella vulgaris 4-hydroxyphenylpyruvate + L-glutamate
-
 r
L-tyrosine + oxaloacetate
-
 Prunella vulgaris 4-hydroxyphenylpyruvate + L-aspartate
-
 r
L-tyrosine + phenylpyruvate
-
 Prunella vulgaris 4-hydroxyphenylpyruvate + L-phenylalanine
-
 r
L-tyrosine + pyruvate
-
 Prunella vulgaris 4-hydroxyphenylpyruvate + L-alanine
-
 r

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Prunella vulgaris

Synonyms

Synonyms Comment Organism
TAT
-
 Prunella vulgaris
tyrosine aminotransferase
-
 Prunella vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Prunella vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 oxaloacetate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.121
-
 4-hydroxyphenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.15
-
 phenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.3
-
 phenylpyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.63
-
 2-oxoglutarate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.73
-
 oxaloacetate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.89
-
 pyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
1.05
-
 L-tryptophan with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
1.19
-
 L-tyrosine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
1.55
-
 L-phenylalanine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Prunella vulgaris

pI Value

Organism Comment pI Value Maximum pI Value
Prunella vulgaris calculated from amino acid sequence
-
 6

General Information

General Information Comment Organism
metabolism the enzyme is involved in rosmarinic acid biosynthesis Prunella vulgaris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 pyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.06
-
 oxaloacetate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.06
-
 2-oxoglutarate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.076
-
 phenylpyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.13
-
 L-tryptophan with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.15
-
 L-phenylalanine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.15
-
 oxaloacetate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.18
-
 phenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
0.295
-
 4-hydroxyphenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
3.03
-
 L-tyrosine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris