Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | the enzyme shows a gradual decrease in the activity till 2.5 M urea, after which the complete loss of activity is measured | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7809 | - |
L-tyrosine | with alpha-ketomethiobutyrate as cosubstrate, at pH 8.0 and 37°C | Leishmania donovani | |
0.8103 | - |
alpha-ketomethiobutyrate | with L-tyrosine as cosubstrate, at pH 8.0 and 37°C | Leishmania donovani | |
3.5 | - |
L-tyrosine | with pyruvate as cosubstrate, at pH 8.0 and 37°C | Leishmania donovani | |
3.572 | - |
L-phenylalanine | with pyruvate as cosubstrate, at pH 8.0 and 37°C | Leishmania donovani | |
5.055 | - |
L-tryptophan | with pyruvate as cosubstrate, at pH 8.0 and 37°C | Leishmania donovani | |
42.67 | - |
pyruvate | with L-tyrosine as cosubstrate, at pH 8.0 and 37°C | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98600 | - |
gel filtration | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | A0A5B8TZA8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + pyruvate | - |
Leishmania donovani | phenylpyruvate + L-alanine | - |
r | |
L-tryptophan + pyruvate | - |
Leishmania donovani | 3-(1H-indol-3-yl)-2-oxopropanoate + L-alanine | - |
r | |
L-tyrosine + alpha-ketomethiobutyrate | - |
Leishmania donovani | ? | - |
r | |
L-tyrosine + pyruvate | - |
Leishmania donovani | 4-hydroxyphenylpyruvate + L-alanine | - |
r | |
additional information | the enzyme exhibits no activity with L-tyrosine and 2-oxoglutarate | Leishmania donovani | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | x * 49300, SDS-PAGE | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
L-tyrosine: 2-oxoglutarate aminotransferase | - |
Leishmania donovani |
TAT | - |
Leishmania donovani |
TATase | - |
Leishmania donovani |
tyrosine aminotransferase | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Leishmania donovani |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | more than 90% activity between 20 and 70°C, about 70% activity at 80°C, about 20% activity at 90°C, no activity at 100°C | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Leishmania donovani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 70% activity between pH 6.0 and 9.0 | Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km value of 0.0235 mM | Leishmania donovani |