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Literature summary for 2.6.1.5 extracted from

  • Sasidharan, S.; Saudagar, P.
    Biochemical and structural characterization of tyrosine aminotransferase suggests broad substrate specificity and a two-state folding mechanism in Leishmania donovani (2019), FEBS Open Bio, 9, 1769-1783 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
Urea the enzyme shows a gradual decrease in the activity till 2.5 M urea, after which the complete loss of activity is measured Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7809
-
L-tyrosine with alpha-ketomethiobutyrate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
0.8103
-
alpha-ketomethiobutyrate with L-tyrosine as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
3.5
-
L-tyrosine with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
3.572
-
L-phenylalanine with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
5.055
-
L-tryptophan with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
42.67
-
pyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 37°C Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98600
-
gel filtration Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani A0A5B8TZA8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + pyruvate
-
Leishmania donovani phenylpyruvate + L-alanine
-
r
L-tryptophan + pyruvate
-
Leishmania donovani 3-(1H-indol-3-yl)-2-oxopropanoate + L-alanine
-
r
L-tyrosine + alpha-ketomethiobutyrate
-
Leishmania donovani ?
-
r
L-tyrosine + pyruvate
-
Leishmania donovani 4-hydroxyphenylpyruvate + L-alanine
-
r
additional information the enzyme exhibits no activity with L-tyrosine and 2-oxoglutarate Leishmania donovani ?
-
-

Subunits

Subunits Comment Organism
homodimer x * 49300, SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
L-tyrosine: 2-oxoglutarate aminotransferase
-
Leishmania donovani
TAT
-
Leishmania donovani
TATase
-
Leishmania donovani
tyrosine aminotransferase
-
Leishmania donovani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Leishmania donovani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 more than 90% activity between 20 and 70°C, about 70% activity at 80°C, about 20% activity at 90°C, no activity at 100°C Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Leishmania donovani

pH Range

pH Minimum pH Maximum Comment Organism
6 9 more than 70% activity between pH 6.0 and 9.0 Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km value of 0.0235 mM Leishmania donovani