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Literature summary for 2.6.1.44 extracted from
- Effects of alanine:glyoxylate aminotransferase variants and pyridoxine sensitivity on oxalate metabolism in a cell-based cytotoxicity assay (2016), Biochim. Biophys. Acta, 1862, 1055-1062.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of an indirect glycolate cytotoxicity assay using CHO cells expressing glycolate oxidase and various normal and mutant forms of AGT | Homo sapiens |
| medicine | the effectiveness of pharmacological doses of pyridoxine results froman improved metabolic effectiveness of AGT. In cells expressing glycolate oxidase the great majority of glycolate is converted to oxalate and glyoxylate, with the latter causing the greater decrease in cell survival. Co-expression of normal AGTs and some, but not all, mutant AGT variants partially counteracts this cytotoxicity and leads to decreased synthesis of oxalate and glyoxylate. Increasing the extracellular pyridoxine up to 0.3 microM leads to an increased metabolic effectiveness of normal AGTs and the G170R variant. The increased survival seen with G170R is paralleled by a 40% decrease in oxalate and glyoxylate levels | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGT | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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