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Literature summary for 2.6.1.43 extracted from

  • Shanker, J.; Datta, K.
    Affinity purification and properties of rat liver mitochondrial L-alanine:4,5-dioxovalerate transaminase and its inhibition by hemin (1986), Arch. Biochem. Biophys., 248, 652-657.
    View publication on PubMed

General Stability

General Stability Organism
purified enzyme is stable in 50 mM potassium buffer, pH 6.9, containing 10% glycerol, freezing and thawing does not affect activity significantly Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
Rattus norvegicus
4,5-Dioxopentanoate
-
Rattus norvegicus
hemin uncompetitive inhibitor Rattus norvegicus
iodoacetate
-
Rattus norvegicus
methylglyoxal
-
Rattus norvegicus
p-chloromercuric benzene sulfonate
-
Rattus norvegicus
pyruvate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
4,5-Dioxopentanoate pH 6.9, 37°C Rattus norvegicus
3.3
-
L-alanine pH 6.9, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
6 * 41000 Rattus norvegicus
232000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 4,5-dioxopentanoate Rattus norvegicus
-
5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate Rattus norvegicus Wistar
-
5-aminolevulinate + pyruvate
-
ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Wistar
-
Rattus norvegicus Wistar
-
Wistar
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.171
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, remains stable for at least 1 month Rattus norvegicus
-70°C, stable for at least 2-3 months Rattus norvegicus
4°C, can be stored for at least 1 week without any significant loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 4,5-dioxopentanoate
-
Rattus norvegicus 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate
-
Rattus norvegicus Wistar 5-aminolevulinate + pyruvate
-
ir
additional information DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine Rattus norvegicus ?
-
?
additional information DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine Rattus norvegicus Wistar ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 41000 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Rattus norvegicus