BRENDA - Enzyme Database show
show all sequences of 2.6.1.43

Purification and properties of L-alanine:4,5-dioxovalerate aminotransferase from Chlorella regularis

Shioi, Y.; Nagamine, M.; Sasa, T.; Arch. Biochem. Biophys. 234, 117-124 (1984)

Data extracted from this reference:

General Stability
General Stability
Organism
stability is improved by 10-15% glycerol
Chlorella regularis
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Chlorella regularis
4,5-Dioxopentanoate
substrate inhibition
Chlorella regularis
amino-oxyacetic acid
-
Chlorella regularis
glyoxylic acid
-
Chlorella regularis
imidazole
-
Chlorella regularis
pyruvate
-
Chlorella regularis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
4,5-Dioxopentanoate
pH 8.0, 37°C
Chlorella regularis
1.3
-
glyoxylate
pH 8.0, 37°C
Chlorella regularis
3
-
L-alanine
pH 8.0, 37°C, glyoxylate as amino acceptor
Chlorella regularis
3.5
-
L-alanine
pH 8.0, 37°C
Chlorella regularis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
2 * 68000, SDS-PAGE
Chlorella regularis
126000
-
gel filtration
Chlorella regularis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Chlorella regularis
-
5-aminolevulinate + pyruvate
-
Chlorella regularis
ir
L-alanine + 4,5-dioxopentanoate
Chlorella regularis S-50
-
5-aminolevulinate + pyruvate
-
Chlorella regularis S-50
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chlorella regularis
-
S-50
-
Chlorella regularis S-50
-
S-50
-
Purification (Commentary)
Commentary
Organism
-
Chlorella regularis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.68
-
-
Chlorella regularis
Storage Stability
Storage Stability
Organism
4°C, 10-15% glycerol, 20% loss of activity after 5 days
Chlorella regularis
4°C, unstable
Chlorella regularis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycine + 4,5-dioxopentanoate
21% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + glyoxylate
-
-
-
ir
glycine + 4,5-dioxopentanoate
21% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + glyoxylate
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640050
Chlorella regularis
5-aminolevulinate + pyruvate
-
640050
Chlorella regularis
ir
L-alanine + 4,5-dioxopentanoate
-
640050
Chlorella regularis S-50
5-aminolevulinate + pyruvate
-
640050
Chlorella regularis S-50
ir
L-glutamate + 4,5-dioxopentanoate
37% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
L-glutamate + 4,5-dioxopentanoate
37% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
L-glutamine + 4,5-dioxopentanoate
16% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 2-oxoglutaramate
-
-
-
-
L-lysine + 4,5-dioxopentanoate
15% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 6-amino-2-oxohexanoate
-
-
-
ir
L-serine + 4,5-dioxopentanoate
33% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
-
-
ir
L-serine + 4,5-dioxopentanoate
33% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
-
-
ir
additional information
does not use 2-oxoglutarate as amino acceptor
640050
Chlorella regularis
?
-
-
-
-
additional information
does not use 2-oxoglutarate as amino acceptor
640050
Chlorella regularis S-50
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 68000, SDS-PAGE
Chlorella regularis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
retains 75% activity after 1 h incubation
Chlorella regularis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Chlorella regularis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.5
-
glyoxylate
pH 8.0, 37°C
Chlorella regularis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Chlorella regularis
isoelectrofocusing column 110 ml and Pharmalyte gradient pH 3-10 at 4°C for 45 h
-
4.8
General Stability (protein specific)
General Stability
Organism
stability is improved by 10-15% glycerol
Chlorella regularis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Chlorella regularis
4,5-Dioxopentanoate
substrate inhibition
Chlorella regularis
amino-oxyacetic acid
-
Chlorella regularis
glyoxylic acid
-
Chlorella regularis
imidazole
-
Chlorella regularis
pyruvate
-
Chlorella regularis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.5
-
glyoxylate
pH 8.0, 37°C
Chlorella regularis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
4,5-Dioxopentanoate
pH 8.0, 37°C
Chlorella regularis
1.3
-
glyoxylate
pH 8.0, 37°C
Chlorella regularis
3
-
L-alanine
pH 8.0, 37°C, glyoxylate as amino acceptor
Chlorella regularis
3.5
-
L-alanine
pH 8.0, 37°C
Chlorella regularis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
2 * 68000, SDS-PAGE
Chlorella regularis
126000
-
gel filtration
Chlorella regularis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Chlorella regularis
-
5-aminolevulinate + pyruvate
-
Chlorella regularis
ir
L-alanine + 4,5-dioxopentanoate
Chlorella regularis S-50
-
5-aminolevulinate + pyruvate
-
Chlorella regularis S-50
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Chlorella regularis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.68
-
-
Chlorella regularis
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 10-15% glycerol, 20% loss of activity after 5 days
Chlorella regularis
4°C, unstable
Chlorella regularis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycine + 4,5-dioxopentanoate
21% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + glyoxylate
-
-
-
ir
glycine + 4,5-dioxopentanoate
21% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + glyoxylate
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640050
Chlorella regularis
5-aminolevulinate + pyruvate
-
640050
Chlorella regularis
ir
L-alanine + 4,5-dioxopentanoate
-
640050
Chlorella regularis S-50
5-aminolevulinate + pyruvate
-
640050
Chlorella regularis S-50
ir
L-glutamate + 4,5-dioxopentanoate
37% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
L-glutamate + 4,5-dioxopentanoate
37% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
L-glutamine + 4,5-dioxopentanoate
16% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 2-oxoglutaramate
-
-
-
-
L-lysine + 4,5-dioxopentanoate
15% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 6-amino-2-oxohexanoate
-
-
-
ir
L-serine + 4,5-dioxopentanoate
33% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
-
-
ir
L-serine + 4,5-dioxopentanoate
33% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
-
-
ir
additional information
does not use 2-oxoglutarate as amino acceptor
640050
Chlorella regularis
?
-
-
-
-
additional information
does not use 2-oxoglutarate as amino acceptor
640050
Chlorella regularis S-50
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 68000, SDS-PAGE
Chlorella regularis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
retains 75% activity after 1 h incubation
Chlorella regularis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Chlorella regularis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Chlorella regularis
isoelectrofocusing column 110 ml and Pharmalyte gradient pH 3-10 at 4°C for 45 h
-
4.8
Other publictions for EC 2.6.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739231
Salgueiro
Effects of Bauhinia forficata ...
Mus musculus
Oxid. Med. Cell. Longev.
2016
0000
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640064
Lombardo
5-Aminolevulinic acid synthesi ...
Trypanosoma cruzi
Int. J. Biochem. Cell Biol.
35
1263-1271
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640063
Sagar
L-alanine: 4,5-dioxovalerate t ...
Leishmania donovani
Microbiol. Res.
150
419-423
1995
-
1
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
640062
Tyagi
A rapid, high-yield purificati ...
Rattus norvegicus, Rattus norvegicus Wistar
Protein Expr. Purif.
5
527-533
1994
-
-
-
-
-
1
1
-
1
-
3
4
-
166
-
-
1
-
-
2
1
1
6
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
3
4
-
-
-
1
-
2
1
1
6
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
640061
Tyagi
In vitro translocation of L-al ...
Rattus norvegicus
J. Biochem.
113
557-562
1993
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640059
McKinney
Production of delta-aminolevul ...
Mus musculus
Int. J. Biochem.
23
803-810
1991
-
-
-
-
-
-
7
2
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640060
Singh
Cytosolic L-alanine:4,5-dioxov ...
Rattus norvegicus, Rattus norvegicus Wistar
Eur. J. Biochem.
198
581-587
1991
1
-
-
-
-
1
1
-
2
-
4
-
-
166
-
-
1
-
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
4
-
-
-
-
1
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640057
Hoare
Characteristics of L-alanine:4 ...
Candida albicans, Candida albicans 3100, Saccharomyces cerevisiae, Saccharomyces cerevisiae 3059 S288C
Arch. Biochem. Biophys.
277
122-129
1990
-
-
-
-
-
1
3
2
2
-
4
4
-
8
-
-
2
-
-
-
1
1
16
2
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
1
-
3
-
2
2
-
4
4
-
-
-
2
-
-
1
1
16
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
640058
McKinney
Production of delta-aminolevul ...
Mus musculus, Mus musculus C57/BL6J
Int. J. Biochem.
22
347-357
1990
-
-
-
-
-
-
-
-
2
-
1
2
-
63
-
-
1
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
-
-
-
1
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640055
Ades
Synthetic substrates for throm ...
Gallus gallus
Int. J. Biochem.
21
579-587
1989
-
-
-
-
-
-
-
2
2
-
1
1
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640056
Prasad
-
Characterization of L-alanine: ...
Cenchrus americanus
Biochem. Int.
18
149-161
1989
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640054
Rhee
L-alanine: 4,5-dioxovalerate a ...
Devosia riboflavina
J. Biochem.
103
1045-1049
1988
-
-
-
1
-
-
3
2
-
-
2
1
-
2
-
-
1
-
-
-
1
1
7
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
2
-
-
2
1
-
-
-
1
-
-
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640053
Shanker
Affinity purification and prop ...
Rattus norvegicus, Rattus norvegicus Wistar
Arch. Biochem. Biophys.
248
652-657
1986
-
-
-
-
-
1
7
2
1
-
2
2
-
166
-
-
1
-
-
1
1
3
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
7
-
2
1
-
2
2
-
-
-
1
-
1
1
3
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640051
Shanker
Evidence of hemin as an end pr ...
Rattus norvegicus, Rattus norvegicus Wistar
FEBS Lett.
189
129-132
1985
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2
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166
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640052
Singh
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
827
305-309
1985
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2
2
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640049
Shioi
-
Purification and characterizat ...
Raphanus sativus
Plant Cell Physiol.
25
1487-1493
1984
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640050
Shioi
Purification and properties of ...
Chlorella regularis, Chlorella regularis S-50
Arch. Biochem. Biophys.
234
117-124
1984
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1
6
4
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2
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7
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12
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1
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12
1
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640048
Bajkowski
delta-Aminolevulinic acid form ...
Clostridium tetanomorphum
J. Biol. Chem.
257
2207-2211
1982
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9
2
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5
1
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3
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640047
Noguchi
Biosynthesis of porphyrin prec ...
Bos taurus
J. Biol. Chem.
256
10335-10339
1981
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5
4
1
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640046
Varticovski
Biosynthesis of porphyrin prec ...
Bos taurus
J. Biol. Chem.
255
3742-3747
1980
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1
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6
2
2
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11
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11
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640045
Neuberger
gamma, delta-Dioxovalerate ami ...
Rhodobacter sphaeroides
Biochim. Biophys. Acta
67
342-345
1963
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25
2
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