BRENDA - Enzyme Database show
show all sequences of 2.6.1.43

delta-Aminolevulinic acid formation. Purification and properties of alanine:4,5-dioxovalerate, aminotransferase and isolation of 4,5-dioxovalerate from Clostridium tetanomorphum

Bajkowski, A.S.; Friedmann, H.C.; J. Biol. Chem. 257, 2207-2211 (1982)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Clostridium tetanomorphum
amino-oxyacetic acid
-
Clostridium tetanomorphum
diacetyl
-
Clostridium tetanomorphum
Glyoxal
-
Clostridium tetanomorphum
glyoxylic acid
-
Clostridium tetanomorphum
methylglyoxal
-
Clostridium tetanomorphum
oxaloacetic acid
-
Clostridium tetanomorphum
pyruvate
-
Clostridium tetanomorphum
succinate semialdehyde
-
Clostridium tetanomorphum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.26
-
4,5-Dioxopentanoate
pH 6.9, 37C
Clostridium tetanomorphum
1.96
-
L-alanine
pH 6.9, 37C
Clostridium tetanomorphum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55500
-
2 * 55500, SDS-PAGE
Clostridium tetanomorphum
72000
-
sedimentation equilibrium centrifugation, potassium phosphate buffer containing 4 M NaCl
Clostridium tetanomorphum
78000
-
sedimentation equilibrium centrifugation, potassium phosphate buffer
Clostridium tetanomorphum
82000
-
gel filtration
Clostridium tetanomorphum
111000
-
gel filtration
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Clostridium tetanomorphum
-
5-aminolevulinate + pyruvate
-
Clostridium tetanomorphum
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium tetanomorphum
-
ATCC 15920
-
Purification (Commentary)
Commentary
Organism
-
Clostridium tetanomorphum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
7.68
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-alanine + 4,5-dioxopentanoate
-
640048
Clostridium tetanomorphum
5-aminolevulinate + pyruvate
-
640048
Clostridium tetanomorphum
ir
L-aspartate + 4,5-dioxopentanoate
67% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + oxaloacetate
-
-
-
ir
L-glutamate + 4,5-dioxopentanoate
96% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
L-histidine + 4,5-dioxopentanoate
74% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
-
-
-
ir
L-phenylalanine + 4,5-dioxopentanoate
71% of the activity with L-alanine
640048
Clostridium tetanomorphum
phenylpyruvate + 5-aminolevulinate
-
-
-
ir
Subunits
Subunits
Commentary
Organism
dimer
2 * 55500, SDS-PAGE
Clostridium tetanomorphum
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Clostridium tetanomorphum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Clostridium tetanomorphum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Clostridium tetanomorphum
amino-oxyacetic acid
-
Clostridium tetanomorphum
diacetyl
-
Clostridium tetanomorphum
Glyoxal
-
Clostridium tetanomorphum
glyoxylic acid
-
Clostridium tetanomorphum
methylglyoxal
-
Clostridium tetanomorphum
oxaloacetic acid
-
Clostridium tetanomorphum
pyruvate
-
Clostridium tetanomorphum
succinate semialdehyde
-
Clostridium tetanomorphum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.26
-
4,5-Dioxopentanoate
pH 6.9, 37C
Clostridium tetanomorphum
1.96
-
L-alanine
pH 6.9, 37C
Clostridium tetanomorphum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55500
-
2 * 55500, SDS-PAGE
Clostridium tetanomorphum
72000
-
sedimentation equilibrium centrifugation, potassium phosphate buffer containing 4 M NaCl
Clostridium tetanomorphum
78000
-
sedimentation equilibrium centrifugation, potassium phosphate buffer
Clostridium tetanomorphum
82000
-
gel filtration
Clostridium tetanomorphum
111000
-
gel filtration
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Clostridium tetanomorphum
-
5-aminolevulinate + pyruvate
-
Clostridium tetanomorphum
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium tetanomorphum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
7.68
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-alanine + 4,5-dioxopentanoate
-
640048
Clostridium tetanomorphum
5-aminolevulinate + pyruvate
-
640048
Clostridium tetanomorphum
ir
L-aspartate + 4,5-dioxopentanoate
67% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + oxaloacetate
-
-
-
ir
L-glutamate + 4,5-dioxopentanoate
96% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
L-histidine + 4,5-dioxopentanoate
74% of the activity with L-alanine
640048
Clostridium tetanomorphum
5-aminolevulinate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
-
-
-
ir
L-phenylalanine + 4,5-dioxopentanoate
71% of the activity with L-alanine
640048
Clostridium tetanomorphum
phenylpyruvate + 5-aminolevulinate
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 55500, SDS-PAGE
Clostridium tetanomorphum
Other publictions for EC 2.6.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739231
Salgueiro
Effects of Bauhinia forficata ...
Mus musculus
Oxid. Med. Cell. Longev.
2016
0000
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
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1
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640064
Lombardo
5-Aminolevulinic acid synthesi ...
Trypanosoma cruzi
Int. J. Biochem. Cell Biol.
35
1263-1271
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640063
Sagar
L-alanine: 4,5-dioxovalerate t ...
Leishmania donovani
Microbiol. Res.
150
419-423
1995
-
1
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
640062
Tyagi
A rapid, high-yield purificati ...
Rattus norvegicus, Rattus norvegicus Wistar
Protein Expr. Purif.
5
527-533
1994
-
-
-
-
-
1
1
-
1
-
3
4
-
166
-
-
1
-
-
2
1
1
6
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
3
4
-
-
-
1
-
2
1
1
6
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
640061
Tyagi
In vitro translocation of L-al ...
Rattus norvegicus
J. Biochem.
113
557-562
1993
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
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1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640059
McKinney
Production of delta-aminolevul ...
Mus musculus
Int. J. Biochem.
23
803-810
1991
-
-
-
-
-
-
7
2
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640060
Singh
Cytosolic L-alanine:4,5-dioxov ...
Rattus norvegicus, Rattus norvegicus Wistar
Eur. J. Biochem.
198
581-587
1991
1
-
-
-
-
1
1
-
2
-
4
-
-
166
-
-
1
-
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
4
-
-
-
-
1
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640057
Hoare
Characteristics of L-alanine:4 ...
Candida albicans, Candida albicans 3100, Saccharomyces cerevisiae, Saccharomyces cerevisiae 3059 S288C
Arch. Biochem. Biophys.
277
122-129
1990
-
-
-
-
-
1
3
2
2
-
4
4
-
8
-
-
2
-
-
-
1
1
16
2
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
1
-
3
-
2
2
-
4
4
-
-
-
2
-
-
1
1
16
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
640058
McKinney
Production of delta-aminolevul ...
Mus musculus, Mus musculus C57/BL6J
Int. J. Biochem.
22
347-357
1990
-
-
-
-
-
-
-
-
2
-
1
2
-
63
-
-
1
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
-
-
-
1
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640055
Ades
Synthetic substrates for throm ...
Gallus gallus
Int. J. Biochem.
21
579-587
1989
-
-
-
-
-
-
-
2
2
-
1
1
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640056
Prasad
-
Characterization of L-alanine: ...
Cenchrus americanus
Biochem. Int.
18
149-161
1989
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
3
-
2
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640054
Rhee
L-alanine: 4,5-dioxovalerate a ...
Devosia riboflavina
J. Biochem.
103
1045-1049
1988
-
-
-
1
-
-
3
2
-
-
2
1
-
2
-
-
1
-
-
-
1
1
7
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
2
-
-
2
1
-
-
-
1
-
-
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640053
Shanker
Affinity purification and prop ...
Rattus norvegicus, Rattus norvegicus Wistar
Arch. Biochem. Biophys.
248
652-657
1986
-
-
-
-
-
1
7
2
1
-
2
2
-
166
-
-
1
-
-
1
1
3
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
7
-
2
1
-
2
2
-
-
-
1
-
1
1
3
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640051
Shanker
Evidence of hemin as an end pr ...
Rattus norvegicus, Rattus norvegicus Wistar
FEBS Lett.
189
129-132
1985
-
-
-
-
-
-
1
-
1
-
-
2
-
166
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640052
Singh
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
827
305-309
1985
-
-
-
-
-
-
2
2
-
-
2
1
-
2
-
-
1
-
-
2
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
2
1
-
-
-
1
-
2
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640049
Shioi
-
Purification and characterizat ...
Raphanus sativus
Plant Cell Physiol.
25
1487-1493
1984
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640050
Shioi
Purification and properties of ...
Chlorella regularis, Chlorella regularis S-50
Arch. Biochem. Biophys.
234
117-124
1984
-
-
-
-
-
1
6
4
-
-
2
2
-
7
-
-
1
-
-
-
1
2
12
1
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
6
1
4
-
-
2
2
-
-
-
1
-
-
1
2
12
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
640048
Bajkowski
delta-Aminolevulinic acid form ...
Clostridium tetanomorphum
J. Biol. Chem.
257
2207-2211
1982
-
-
-
-
-
-
9
2
-
-
5
1
-
3
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
9
-
2
-
-
5
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640047
Noguchi
Biosynthesis of porphyrin prec ...
Bos taurus
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1981
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5
4
1
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4
1
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1
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1
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1
1
2
3
1
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1
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5
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1
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4
1
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1
1
2
3
1
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1
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640046
Varticovski
Biosynthesis of porphyrin prec ...
Bos taurus
J. Biol. Chem.
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3742-3747
1980
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1
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1
6
2
2
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1
1
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1
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1
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1
1
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11
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1
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1
1
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6
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2
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1
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1
1
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11
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1
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640045
Neuberger
gamma, delta-Dioxovalerate ami ...
Rhodobacter sphaeroides
Biochim. Biophys. Acta
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342-345
1963
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1
25
2
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7
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25
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7
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