BRENDA - Enzyme Database show
show all sequences of 2.6.1.43

Biosynthesis of porphyrin precursors. Purification and characterization of mammalian L-alanine:gamma,delta-dioxovaleric acid aminotransferase

Varticovski, L.; Kushner, J.P.; Burnham, B.F.; J. Biol. Chem. 255, 3742-3747 (1980)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Bos taurus
General Stability
General Stability
Organism
freezing and thawing does not affect activity
Bos taurus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Bos taurus
5-aminolevulinate
-
Bos taurus
methylglyoxal
-
Bos taurus
pyruvate
-
Bos taurus
succinate semialdehyde
-
Bos taurus
Tris-HCl
-
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
4,5-Dioxopentanoate
pH 6.9, 37°C
Bos taurus
3.7
-
L-alanine
pH 6.9, 37°C
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
cytosol contains minimal enzymic activity
Bos taurus
5829
-
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
240000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Bos taurus
-
5-aminolevulinate + pyruvate
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
bovine
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.16
-
-
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-alanine + 4,5-dioxopentanoate
23% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
D-alanine + 4,5-dioxopentanoate
3% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
delta-amino-n-valerate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-oxo-n-pentanoate
-
-
-
ir
DL-alanine + 4,5-dioxopentanoate
89% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
glycine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + glyoxylate
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640046
Bos taurus
5-aminolevulinate + pyruvate
-
640046
Bos taurus
ir
L-lysine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 6-amino-2-oxohexanoate
-
-
-
ir
L-ornithine + 4,5-dioxopentanoate
6% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
additional information
glutamate is no substrate, transamination of methylglyoxal yields aminoacetone, less than 1% efficient as amino acceptor as 4,5-dioxopentanoate
640046
Bos taurus
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
-
-
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Bos taurus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Bos taurus
General Stability (protein specific)
General Stability
Organism
freezing and thawing does not affect activity
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Bos taurus
5-aminolevulinate
-
Bos taurus
methylglyoxal
-
Bos taurus
pyruvate
-
Bos taurus
succinate semialdehyde
-
Bos taurus
Tris-HCl
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
4,5-Dioxopentanoate
pH 6.9, 37°C
Bos taurus
3.7
-
L-alanine
pH 6.9, 37°C
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
cytosol contains minimal enzymic activity
Bos taurus
5829
-
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
240000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Bos taurus
-
5-aminolevulinate + pyruvate
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.16
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-alanine + 4,5-dioxopentanoate
23% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
D-alanine + 4,5-dioxopentanoate
3% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
delta-amino-n-valerate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-oxo-n-pentanoate
-
-
-
ir
DL-alanine + 4,5-dioxopentanoate
89% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
glycine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + glyoxylate
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640046
Bos taurus
5-aminolevulinate + pyruvate
-
640046
Bos taurus
ir
L-lysine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 6-amino-2-oxohexanoate
-
-
-
ir
L-ornithine + 4,5-dioxopentanoate
6% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
additional information
glutamate is no substrate, transamination of methylglyoxal yields aminoacetone, less than 1% efficient as amino acceptor as 4,5-dioxopentanoate
640046
Bos taurus
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
-
-
Bos taurus
Other publictions for EC 2.6.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739231
Salgueiro
Effects of Bauhinia forficata ...
Mus musculus
Oxid. Med. Cell. Longev.
2016
0000
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640064
Lombardo
5-Aminolevulinic acid synthesi ...
Trypanosoma cruzi
Int. J. Biochem. Cell Biol.
35
1263-1271
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640063
Sagar
L-alanine: 4,5-dioxovalerate t ...
Leishmania donovani
Microbiol. Res.
150
419-423
1995
-
1
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
640062
Tyagi
A rapid, high-yield purificati ...
Rattus norvegicus, Rattus norvegicus Wistar
Protein Expr. Purif.
5
527-533
1994
-
-
-
-
-
1
1
-
1
-
3
4
-
166
-
-
1
-
-
2
1
1
6
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
3
4
-
-
-
1
-
2
1
1
6
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
640061
Tyagi
In vitro translocation of L-al ...
Rattus norvegicus
J. Biochem.
113
557-562
1993
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640059
McKinney
Production of delta-aminolevul ...
Mus musculus
Int. J. Biochem.
23
803-810
1991
-
-
-
-
-
-
7
2
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640060
Singh
Cytosolic L-alanine:4,5-dioxov ...
Rattus norvegicus, Rattus norvegicus Wistar
Eur. J. Biochem.
198
581-587
1991
1
-
-
-
-
1
1
-
2
-
4
-
-
166
-
-
1
-
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
4
-
-
-
-
1
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640057
Hoare
Characteristics of L-alanine:4 ...
Candida albicans, Candida albicans 3100, Saccharomyces cerevisiae, Saccharomyces cerevisiae 3059 S288C
Arch. Biochem. Biophys.
277
122-129
1990
-
-
-
-
-
1
3
2
2
-
4
4
-
8
-
-
2
-
-
-
1
1
16
2
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
1
-
3
-
2
2
-
4
4
-
-
-
2
-
-
1
1
16
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
640058
McKinney
Production of delta-aminolevul ...
Mus musculus, Mus musculus C57/BL6J
Int. J. Biochem.
22
347-357
1990
-
-
-
-
-
-
-
-
2
-
1
2
-
63
-
-
1
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
-
-
-
1
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640055
Ades
Synthetic substrates for throm ...
Gallus gallus
Int. J. Biochem.
21
579-587
1989
-
-
-
-
-
-
-
2
2
-
1
1
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640056
Prasad
-
Characterization of L-alanine: ...
Cenchrus americanus
Biochem. Int.
18
149-161
1989
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640054
Rhee
L-alanine: 4,5-dioxovalerate a ...
Devosia riboflavina
J. Biochem.
103
1045-1049
1988
-
-
-
1
-
-
3
2
-
-
2
1
-
2
-
-
1
-
-
-
1
1
7
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
2
-
-
2
1
-
-
-
1
-
-
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640053
Shanker
Affinity purification and prop ...
Rattus norvegicus, Rattus norvegicus Wistar
Arch. Biochem. Biophys.
248
652-657
1986
-
-
-
-
-
1
7
2
1
-
2
2
-
166
-
-
1
-
-
1
1
3
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
7
-
2
1
-
2
2
-
-
-
1
-
1
1
3
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640051
Shanker
Evidence of hemin as an end pr ...
Rattus norvegicus, Rattus norvegicus Wistar
FEBS Lett.
189
129-132
1985
-
-
-
-
-
-
1
-
1
-
-
2
-
166
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640052
Singh
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
827
305-309
1985
-
-
-
-
-
-
2
2
-
-
2
1
-
2
-
-
1
-
-
2
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
2
1
-
-
-
1
-
2
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640049
Shioi
-
Purification and characterizat ...
Raphanus sativus
Plant Cell Physiol.
25
1487-1493
1984
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
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1
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1
1
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1
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1
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1
2
12
1
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1
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1
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1
1
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1
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6
1
4
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2
2
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1
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1
2
12
1
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1
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1
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1
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1
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1
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5
1
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1
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1
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9
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2
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5
1
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1
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5
1
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1
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1
1
2
3
1
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1
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5
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1
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4
1
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1
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1
1
2
3
1
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1
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1
1
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11
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1
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1
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1
1
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1
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6
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2
2
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1
1
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1
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1
1
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11
-
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1
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7
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1
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1
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25
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7
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