Data extracted from this reference:
Crystallization (Commentary)
General Stability
freezing and thawing does not affect activity
Bos taurus
Inhibitors
2-oxoglutarate
Bos taurus
5-aminolevulinate
Bos taurus
succinate semialdehyde
Bos taurus
KM Value [mM]
0.24
4,5-Dioxopentanoate
pH 6.9, 37°C
Bos taurus
3.7
L-alanine
pH 6.9, 37°C
Bos taurus
Localization
cytosol
cytosol contains minimal enzymic activity
Bos taurus
5829
mitochondrion
Bos taurus
5739
Molecular Weight [Da]
240000
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
L-alanine + 4,5-dioxopentanoate
Bos taurus
5-aminolevulinate + pyruvate
ir
Organism
Purification (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Substrates and Products (Substrate)
beta-alanine + 4,5-dioxopentanoate
23% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + malonic semialdehyde
ir
D-alanine + 4,5-dioxopentanoate
3% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
ir
delta-amino-n-valerate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-oxo-n-pentanoate
ir
DL-alanine + 4,5-dioxopentanoate
89% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + succinic semialdehyde
ir
glycine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + glyoxylate
ir
L-alanine + 4,5-dioxopentanoate
640046
Bos taurus
5-aminolevulinate + pyruvate
ir
L-alanine + 4,5-dioxopentanoate
640046
Bos taurus
5-aminolevulinate + pyruvate
640046
Bos taurus
ir
L-lysine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 6-amino-2-oxohexanoate
ir
L-ornithine + 4,5-dioxopentanoate
6% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-amino-2-oxopentanoate
ir
additional information
glutamate is no substrate, transamination of methylglyoxal yields aminoacetone, less than 1% efficient as amino acceptor as 4,5-dioxopentanoate
640046
Bos taurus
?
pH Optimum
Cofactor
pyridoxal 5'-phosphate
Bos taurus
Cofactor (protein specific)
pyridoxal 5'-phosphate
Bos taurus
Crystallization (Commentary) (protein specific)
General Stability (protein specific)
freezing and thawing does not affect activity
Bos taurus
Inhibitors (protein specific)
2-oxoglutarate
Bos taurus
5-aminolevulinate
Bos taurus
succinate semialdehyde
Bos taurus
KM Value [mM] (protein specific)
0.24
4,5-Dioxopentanoate
pH 6.9, 37°C
Bos taurus
3.7
L-alanine
pH 6.9, 37°C
Bos taurus
Localization (protein specific)
cytosol
cytosol contains minimal enzymic activity
Bos taurus
5829
mitochondrion
Bos taurus
5739
Molecular Weight [Da] (protein specific)
240000
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
L-alanine + 4,5-dioxopentanoate
Bos taurus
5-aminolevulinate + pyruvate
ir
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Substrates and Products (Substrate) (protein specific)
beta-alanine + 4,5-dioxopentanoate
23% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + malonic semialdehyde
ir
D-alanine + 4,5-dioxopentanoate
3% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
ir
delta-amino-n-valerate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-oxo-n-pentanoate
ir
DL-alanine + 4,5-dioxopentanoate
89% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + succinic semialdehyde
ir
glycine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + glyoxylate
ir
L-alanine + 4,5-dioxopentanoate
640046
Bos taurus
5-aminolevulinate + pyruvate
ir
L-alanine + 4,5-dioxopentanoate
640046
Bos taurus
5-aminolevulinate + pyruvate
640046
Bos taurus
ir
L-lysine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 6-amino-2-oxohexanoate
ir
L-ornithine + 4,5-dioxopentanoate
6% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-amino-2-oxopentanoate
ir
additional information
glutamate is no substrate, transamination of methylglyoxal yields aminoacetone, less than 1% efficient as amino acceptor as 4,5-dioxopentanoate
640046
Bos taurus
?
pH Optimum (protein specific)
Other publictions for EC 2.6.1.43
739231
Salgueiro
Effects of Bauhinia forficata ...
Mus musculus
Oxid. Med. Cell. Longev.
2016
0000
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640064
Lombardo
5-Aminolevulinic acid synthesi ...
Trypanosoma cruzi
Int. J. Biochem. Cell Biol.
35
1263-1271
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640063
Sagar
L-alanine: 4,5-dioxovalerate t ...
Leishmania donovani
Microbiol. Res.
150
419-423
1995
-
1
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
640062
Tyagi
A rapid, high-yield purificati ...
Rattus norvegicus, Rattus norvegicus Wistar
Protein Expr. Purif.
5
527-533
1994
-
-
-
-
-
1
1
-
1
-
3
4
-
166
-
-
1
-
-
2
1
1
6
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
3
4
-
-
-
1
-
2
1
1
6
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
640061
Tyagi
In vitro translocation of L-al ...
Rattus norvegicus
J. Biochem.
113
557-562
1993
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640059
McKinney
Production of delta-aminolevul ...
Mus musculus
Int. J. Biochem.
23
803-810
1991
-
-
-
-
-
-
7
2
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640060
Singh
Cytosolic L-alanine:4,5-dioxov ...
Rattus norvegicus, Rattus norvegicus Wistar
Eur. J. Biochem.
198
581-587
1991
1
-
-
-
-
1
1
-
2
-
4
-
-
166
-
-
1
-
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
4
-
-
-
-
1
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640057
Hoare
Characteristics of L-alanine:4 ...
Candida albicans, Candida albicans 3100, Saccharomyces cerevisiae, Saccharomyces cerevisiae 3059 S288C
Arch. Biochem. Biophys.
277
122-129
1990
-
-
-
-
-
1
3
2
2
-
4
4
-
8
-
-
2
-
-
-
1
1
16
2
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
1
-
3
-
2
2
-
4
4
-
-
-
2
-
-
1
1
16
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
640058
McKinney
Production of delta-aminolevul ...
Mus musculus, Mus musculus C57/BL6J
Int. J. Biochem.
22
347-357
1990
-
-
-
-
-
-
-
-
2
-
1
2
-
63
-
-
1
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
-
-
-
1
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640055
Ades
Synthetic substrates for throm ...
Gallus gallus
Int. J. Biochem.
21
579-587
1989
-
-
-
-
-
-
-
2
2
-
1
1
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640056
Prasad
-
Characterization of L-alanine: ...
Cenchrus americanus
Biochem. Int.
18
149-161
1989
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640054
Rhee
L-alanine: 4,5-dioxovalerate a ...
Devosia riboflavina
J. Biochem.
103
1045-1049
1988
-
-
-
1
-
-
3
2
-
-
2
1
-
2
-
-
1
-
-
-
1
1
7
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
2
-
-
2
1
-
-
-
1
-
-
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640053
Shanker
Affinity purification and prop ...
Rattus norvegicus, Rattus norvegicus Wistar
Arch. Biochem. Biophys.
248
652-657
1986
-
-
-
-
-
1
7
2
1
-
2
2
-
166
-
-
1
-
-
1
1
3
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
7
-
2
1
-
2
2
-
-
-
1
-
1
1
3
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640051
Shanker
Evidence of hemin as an end pr ...
Rattus norvegicus, Rattus norvegicus Wistar
FEBS Lett.
189
129-132
1985
-
-
-
-
-
-
1
-
1
-
-
2
-
166
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640052
Singh
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
827
305-309
1985
-
-
-
-
-
-
2
2
-
-
2
1
-
2
-
-
1
-
-
2
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
2
1
-
-
-
1
-
2
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640049
Shioi
-
Purification and characterizat ...
Raphanus sativus
Plant Cell Physiol.
25
1487-1493
1984
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640050
Shioi
Purification and properties of ...
Chlorella regularis, Chlorella regularis S-50
Arch. Biochem. Biophys.
234
117-124
1984
-
-
-
-
-
1
6
4
-
-
2
2
-
7
-
-
1
-
-
-
1
2
12
1
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
6
1
4
-
-
2
2
-
-
-
1
-
-
1
2
12
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
640048
Bajkowski
delta-Aminolevulinic acid form ...
Clostridium tetanomorphum
J. Biol. Chem.
257
2207-2211
1982
-
-
-
-
-
-
9
2
-
-
5
1
-
3
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
9
-
2
-
-
5
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640047
Noguchi
Biosynthesis of porphyrin prec ...
Bos taurus
J. Biol. Chem.
256
10335-10339
1981
-
-
-
-
-
-
5
4
1
-
4
1
-
1
-
-
1
-
-
1
1
2
3
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
4
1
-
4
1
-
-
-
1
-
1
1
2
3
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
640046
Varticovski
Biosynthesis of porphyrin prec ...
Bos taurus
J. Biol. Chem.
255
3742-3747
1980
-
-
-
1
-
1
6
2
2
-
1
1
-
1
-
-
1
-
-
1
1
-
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
1
-
6
-
2
2
-
1
1
-
-
-
1
-
1
1
-
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640045
Neuberger
gamma, delta-Dioxovalerate ami ...
Rhodobacter sphaeroides
Biochim. Biophys. Acta
67
342-345
1963
-
-
-
-
-
1
25
2
-
-
-
1
-
2
-
-
1
-
-
-
-
1
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
25
-
2
-
-
-
1
-
-
-
1
-
-
-
1
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-