BRENDA - Enzyme Database show
show all sequences of 2.6.1.43

gamma, delta-Dioxovalerate aminotransferase activity in Rhodopseudomonas spheroides

Neuberger, A.; Turner, J.M.; Biochim. Biophys. Acta 67, 342-345 (1963)

Data extracted from this reference:

General Stability
General Stability
Organism
glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM
Rhodobacter sphaeroides
Inhibitors
Inhibitors
Commentary
Organism
Structure
4,5-Dioxopentanoate
substrate inhibition
Rhodobacter sphaeroides
acetyl acetone
-
Rhodobacter sphaeroides
amino-oxyacetic acid
-
Rhodobacter sphaeroides
benzoyl acetone
-
Rhodobacter sphaeroides
beta-Alanine
8% inhibition
Rhodobacter sphaeroides
beta-Iodopropionate
30% inhibition
Rhodobacter sphaeroides
cadaverine
14% inhibition
Rhodobacter sphaeroides
cysteamine
-
Rhodobacter sphaeroides
cysteine
90% inhibition
Rhodobacter sphaeroides
diacetyl
-
Rhodobacter sphaeroides
ethyl acetoacetate
-
Rhodobacter sphaeroides
glyoxylic acid
-
Rhodobacter sphaeroides
homocysteine
-
Rhodobacter sphaeroides
iodoacetate
30% inhibition
Rhodobacter sphaeroides
Isonicotinic acid hydrazide
-
Rhodobacter sphaeroides
lysine
20% inhibition
Rhodobacter sphaeroides
additional information
iodoacetamide and chloroacetamide are not inhibitors
Rhodobacter sphaeroides
ophthalaldehyde
-
Rhodobacter sphaeroides
ornithine
20% inhibition
Rhodobacter sphaeroides
oxaloacetic acid
-
Rhodobacter sphaeroides
p-chloromercuribenzoate
78% inhibition
Rhodobacter sphaeroides
Penicillamine
-
Rhodobacter sphaeroides
Pyruvic acid
-
Rhodobacter sphaeroides
succinic acid
-
Rhodobacter sphaeroides
Thioglycollate
74% inhibition
Rhodobacter sphaeroides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
4,5-Dioxopentanoate
pH 7.0, 37°C
Rhodobacter sphaeroides
8
-
L-alanine
pH 7.0, 37°C
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Rhodobacter sphaeroides
-
5-aminolevulinate + pyruvate
-
Rhodobacter sphaeroides
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rhodobacter sphaeroides
Storage Stability
Storage Stability
Organism
-15°C, stable for several months
Rhodobacter sphaeroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
delta-amino-n-valerate + 4,5-dioxopentanoate
transaminate at 95% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 5-oxo-n-pentanoate
-
-
-
ir
epsilon-amino-n-caproate + 4,5-dioxopentanoate
transaminate at 103% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 6-oxo-n-hexanoate
-
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
transaminate at 122% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
glycine + 4,5-dioxopentanoate
less than 10% of the activity of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + glyoxylate
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640045
Rhodobacter sphaeroides
5-aminolevulinate + pyruvate
-
640045
Rhodobacter sphaeroides
ir
L-ornithine + 4,5-dioxopentanoate
less than 10% of the activity of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
additional information
ability of beta-alanine to replace alpha-alanine as amino group donor is lost during purification, alpha-amino-n-butyrate, alpha-aminoisobutyrate, beta-amino-n-butyrate and beta-aminoisobutyrate are inactive, serine, taurine, glutamine, asparagine, glutamic and aspartic acids, lysine and cysteine all have negligible activity
640045
Rhodobacter sphaeroides
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Rhodobacter sphaeroides
General Stability (protein specific)
General Stability
Organism
glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM
Rhodobacter sphaeroides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4,5-Dioxopentanoate
substrate inhibition
Rhodobacter sphaeroides
acetyl acetone
-
Rhodobacter sphaeroides
amino-oxyacetic acid
-
Rhodobacter sphaeroides
benzoyl acetone
-
Rhodobacter sphaeroides
beta-Alanine
8% inhibition
Rhodobacter sphaeroides
beta-Iodopropionate
30% inhibition
Rhodobacter sphaeroides
cadaverine
14% inhibition
Rhodobacter sphaeroides
cysteamine
-
Rhodobacter sphaeroides
cysteine
90% inhibition
Rhodobacter sphaeroides
diacetyl
-
Rhodobacter sphaeroides
ethyl acetoacetate
-
Rhodobacter sphaeroides
glyoxylic acid
-
Rhodobacter sphaeroides
homocysteine
-
Rhodobacter sphaeroides
iodoacetate
30% inhibition
Rhodobacter sphaeroides
Isonicotinic acid hydrazide
-
Rhodobacter sphaeroides
lysine
20% inhibition
Rhodobacter sphaeroides
additional information
iodoacetamide and chloroacetamide are not inhibitors
Rhodobacter sphaeroides
ophthalaldehyde
-
Rhodobacter sphaeroides
ornithine
20% inhibition
Rhodobacter sphaeroides
oxaloacetic acid
-
Rhodobacter sphaeroides
p-chloromercuribenzoate
78% inhibition
Rhodobacter sphaeroides
Penicillamine
-
Rhodobacter sphaeroides
Pyruvic acid
-
Rhodobacter sphaeroides
succinic acid
-
Rhodobacter sphaeroides
Thioglycollate
74% inhibition
Rhodobacter sphaeroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
4,5-Dioxopentanoate
pH 7.0, 37°C
Rhodobacter sphaeroides
8
-
L-alanine
pH 7.0, 37°C
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-alanine + 4,5-dioxopentanoate
Rhodobacter sphaeroides
-
5-aminolevulinate + pyruvate
-
Rhodobacter sphaeroides
ir
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rhodobacter sphaeroides
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, stable for several months
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
delta-amino-n-valerate + 4,5-dioxopentanoate
transaminate at 95% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 5-oxo-n-pentanoate
-
-
-
ir
epsilon-amino-n-caproate + 4,5-dioxopentanoate
transaminate at 103% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 6-oxo-n-hexanoate
-
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
transaminate at 122% of the rate of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
glycine + 4,5-dioxopentanoate
less than 10% of the activity of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + glyoxylate
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
-
640045
Rhodobacter sphaeroides
5-aminolevulinate + pyruvate
-
640045
Rhodobacter sphaeroides
ir
L-ornithine + 4,5-dioxopentanoate
less than 10% of the activity of alpha-alanine
640045
Rhodobacter sphaeroides
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
additional information
ability of beta-alanine to replace alpha-alanine as amino group donor is lost during purification, alpha-amino-n-butyrate, alpha-aminoisobutyrate, beta-amino-n-butyrate and beta-aminoisobutyrate are inactive, serine, taurine, glutamine, asparagine, glutamic and aspartic acids, lysine and cysteine all have negligible activity
640045
Rhodobacter sphaeroides
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Rhodobacter sphaeroides
Other publictions for EC 2.6.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739231
Salgueiro
Effects of Bauhinia forficata ...
Mus musculus
Oxid. Med. Cell. Longev.
2016
0000
2016
-
1
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640064
Lombardo
5-Aminolevulinic acid synthesi ...
Trypanosoma cruzi
Int. J. Biochem. Cell Biol.
35
1263-1271
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
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1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640063
Sagar
L-alanine: 4,5-dioxovalerate t ...
Leishmania donovani
Microbiol. Res.
150
419-423
1995
-
1
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
640062
Tyagi
A rapid, high-yield purificati ...
Rattus norvegicus, Rattus norvegicus Wistar
Protein Expr. Purif.
5
527-533
1994
-
-
-
-
-
1
1
-
1
-
3
4
-
166
-
-
1
-
-
2
1
1
6
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
3
4
-
-
-
1
-
2
1
1
6
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
640061
Tyagi
In vitro translocation of L-al ...
Rattus norvegicus
J. Biochem.
113
557-562
1993
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640059
McKinney
Production of delta-aminolevul ...
Mus musculus
Int. J. Biochem.
23
803-810
1991
-
-
-
-
-
-
7
2
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640060
Singh
Cytosolic L-alanine:4,5-dioxov ...
Rattus norvegicus, Rattus norvegicus Wistar
Eur. J. Biochem.
198
581-587
1991
1
-
-
-
-
1
1
-
2
-
4
-
-
166
-
-
1
-
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
4
-
-
-
-
1
-
2
1
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640057
Hoare
Characteristics of L-alanine:4 ...
Candida albicans, Candida albicans 3100, Saccharomyces cerevisiae, Saccharomyces cerevisiae 3059 S288C
Arch. Biochem. Biophys.
277
122-129
1990
-
-
-
-
-
1
3
2
2
-
4
4
-
8
-
-
2
-
-
-
1
1
16
2
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
1
-
3
-
2
2
-
4
4
-
-
-
2
-
-
1
1
16
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
640058
McKinney
Production of delta-aminolevul ...
Mus musculus, Mus musculus C57/BL6J
Int. J. Biochem.
22
347-357
1990
-
-
-
-
-
-
-
-
2
-
1
2
-
63
-
-
1
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
-
-
-
1
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640055
Ades
Synthetic substrates for throm ...
Gallus gallus
Int. J. Biochem.
21
579-587
1989
-
-
-
-
-
-
-
2
2
-
1
1
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640056
Prasad
-
Characterization of L-alanine: ...
Cenchrus americanus
Biochem. Int.
18
149-161
1989
-
-
-
-
-
-
3
2
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640054
Rhee
L-alanine: 4,5-dioxovalerate a ...
Devosia riboflavina
J. Biochem.
103
1045-1049
1988
-
-
-
1
-
-
3
2
-
-
2
1
-
2
-
-
1
-
-
-
1
1
7
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
2
-
-
2
1
-
-
-
1
-
-
1
1
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640053
Shanker
Affinity purification and prop ...
Rattus norvegicus, Rattus norvegicus Wistar
Arch. Biochem. Biophys.
248
652-657
1986
-
-
-
-
-
1
7
2
1
-
2
2
-
166
-
-
1
-
-
1
1
3
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
7
-
2
1
-
2
2
-
-
-
1
-
1
1
3
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640051
Shanker
Evidence of hemin as an end pr ...
Rattus norvegicus, Rattus norvegicus Wistar
FEBS Lett.
189
129-132
1985
-
-
-
-
-
-
1
-
1
-
-
2
-
166
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640052
Singh
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
827
305-309
1985
-
-
-
-
-
-
2
2
-
-
2
1
-
1
-
-
1
-
-
1
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
2
1
-
-
-
1
-
1
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640049
Shioi
-
Purification and characterizat ...
Raphanus sativus
Plant Cell Physiol.
25
1487-1493
1984
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
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1
1
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1
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6
4
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2
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7
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12
1
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6
1
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2
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1
2
12
1
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1
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9
2
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1
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3
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5
1
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9
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1
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1
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5
1
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5
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1
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4
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1
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3
1
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5
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1
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1
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1
2
3
1
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1
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1
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1
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11
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1
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1
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1
1
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1
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6
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2
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1
1
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1
1
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11
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1
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640045
Neuberger
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25
2
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25
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