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Literature summary for 2.6.1.42 extracted from

  • Weinhandl, K.; Ballach, M.; Winkler, M.; Ahmad, M.; Glieder, A.; Birner-Gruenberger, R.; Fotheringham, I.; Escalettes, F.; Camattari, A.
    Pichia pastoris mutants as host strains for efficient secretion of recombinant branched chain aminotransferase (BCAT) (2016), J. Biotechnol., 235, 84-91 .
    View publication on PubMed
Search for more literature for 2.6.1.42

Cloned(Commentary)

Cloned (Comment) Organism
gene BCAT, recombinant expression in a Pichia pastoris strains (DELTAcwp1, DELTAscw10 resulting in deletio of cell wall proteins) OCH1 knockout strain FWK3, the recombinant Escherichia coli enzyme is secreted. Method development and optimization, detailed overview Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information Pichia pastoris strain CBS7435 MutS is used as parent strain for BCAT expressions. The improved BCAT secretion by OCH1 knockout in Pichia pastoris, provides a good system for efficient chiral amine production. Method improvement leads to increase of the extracellular BCAT production, but also to achieve a more homogenous product in terms of glycosylation, identification of a deletion strain that counteracts typical cell clustering in the Pichia pastoris DELTAoch1 strain. The knockout of the locus OCH1, strain FWK3, leads to a more homogenous glycosylation: more Man8 than Man10 N-glycans are added during the mannosylation process, facilitating for example the handling for downstream processes, since hypermannosylation and non homogenous glycosylation can be largely avoided Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-leucine + 2-oxoglutarate Escherichia coli
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AB80
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine + 2-oxoglutarate
-
 Escherichia coli 4-methyl-2-oxopentanoate + L-glutamate
-
 r

Synonyms

Synonyms Comment Organism
BcaT
-
 Escherichia coli
branched chain aminotransferase
-
 Escherichia coli
IlvE
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP Escherichia coli