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Literature summary for 2.6.1.42 extracted from

  • Uchida, Y.; Hayashi, H.; Washio, T.; Yamasaki, R.; Kato, S.; Oikawa, T.
    Cloning and characterization of a novel fold-type I branched-chain amino acid aminotransferase from the hyperthermophilic archaeon Thermococcus sp. CKU-1 (2014), Extremophiles, 18, 589-602.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate residual activity 79% Thermococcus sp.
DL-Penicillamine residual activity 79% Thermococcus sp.
hydroxylamine residual activity 72% Thermococcus sp.
Semicarbazide residual activity 95% Thermococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
2-oxo-3-methylvalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.06
-
2-oxoisocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.06
-
2-oxocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.15
-
L-isoleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.16
-
2-oxovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.18
-
2-oxoisovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.34
-
phenylpyruvate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.55
-
2-oxobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.73
-
DL-2-aminooctanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
0.83
-
L-valine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
1.2
-
L-leucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
1.7
-
2-oxoglutarate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
1.9
-
L-norvaline half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2
-
DL-2-aminoheptanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
2.4
-
L-2-aminobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
3.9
-
L-glutamate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
4.2
-
L-phenylalanine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
12
-
L-norleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.

Organism

Organism UniProt Comment Textmining
Thermococcus sp. A0A060PQX5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-methylvalerate + glyoxalate
-
Thermococcus sp. L-isoleucine + glycine
-
r
DL-2-aminoheptanoate + 2-oxoglutarate
-
Thermococcus sp. 2-oxoheptanoate + L-glutamate
-
r
DL-2-aminooctanoate + 2-oxobutanoate
-
Thermococcus sp. 2-oxooctanoate + L-2-aminobutanoate
-
r
L-2-aminobutanoate + phenylpyruvate
-
Thermococcus sp. 2-oxobutanoate + L-phenylalanine
-
r
L-glutamate + glyoxalate
-
Thermococcus sp. 2-oxoglutarate + glycine
-
r
L-isoleucine + 2-oxoisocaproate
-
Thermococcus sp. 3-methyl-2-oxopentanoate + L-leucine
-
r
L-leucine + 2-oxobutanoate
-
Thermococcus sp. 2-oxoisohexanoate + L-valine
-
r
L-norleucine + 2-oxoisocaproate
-
Thermococcus sp. 2-oxohexanoate + L-leucine
-
r
L-norvaline + 2-oxocaproate
-
Thermococcus sp. 2-oxopentanoate + 2-aminohexanoate
-
r
L-phenylalanine + 2-oxovalerate
-
Thermococcus sp. phenylpyruvate + L-norvaline
-
r
L-valine + 2-oxoisovalerate
-
Thermococcus sp. 2-oxoisovalerate + L-leucine
-
r

Synonyms

Synonyms Comment Organism
BcaT
-
Thermococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Thermococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
stable for at least 5 h Thermococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
L-2-aminobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
27
-
DL-2-aminoheptanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
31
-
L-valine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
51
-
L-isoleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
52
-
phenylpyruvate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
80
-
L-phenylalanine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
81
-
DL-2-aminooctanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
86
-
L-norvaline half transamination reaction, pH 8.0, 90°C Thermococcus sp.
91
-
2-oxocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
110
-
L-leucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
150
-
L-glutamate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
190
-
2-oxovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
250
-
2-oxobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
260
-
L-norleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
270
-
2-oxoisovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
270
-
2-oxoisocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
360
-
2-oxo-3-methylvalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
700
-
2-oxoglutarate half transamination reaction, pH 8.0, 90°C Thermococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Thermococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7
-
Thermococcus sp.

General Information

General Information Comment Organism
physiological function Thermococcus sp. CKU-1 requires L-leucine, L-methionine, L-phenylalanine, L-threonine, L-tryptophan, and L-tyrosine for its growth, which are good substrates for BcAT. The enzyme might be involved in the degradation of these amino acids Thermococcus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11
-
L-2-aminobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
13
-
DL-2-aminoheptanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
19
-
L-phenylalanine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
21
-
L-norleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
38
-
L-valine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
39
-
L-glutamate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
45
-
L-norvaline half transamination reaction, pH 8.0, 90°C Thermococcus sp.
87
-
L-leucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
110
-
DL-2-aminooctanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
150
-
phenylpyruvate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
350
-
L-isoleucine half transamination reaction, pH 8.0, 90°C Thermococcus sp.
430
-
2-oxoglutarate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
460
-
2-oxobutanoate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
1200
-
2-oxovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
1500
-
2-oxoisovalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
1500
-
2-oxocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
4600
-
2-oxoisocaproate half transamination reaction, pH 8.0, 90°C Thermococcus sp.
11000
-
2-oxo-3-methylvalerate half transamination reaction, pH 8.0, 90°C Thermococcus sp.