Cloned (Comment) | Organism |
---|---|
glutathione S-transferase tagged protein expressed in Escherichia coli BL21(DE3) | Solanum lycopersicum |
glutathione S-transferase tagged protein expressed in Escherichia coli BL21(DE3) | Solanum pennellii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-leucine | pH not specified in the publication, 25°C | Solanum lycopersicum | |
0.31 | - |
L-isoleucine | pH not specified in the publication, 25°C | Solanum lycopersicum | |
0.35 | - |
L-valine | pH not specified in the publication, 25°C | Solanum lycopersicum | |
0.56 | - |
L-valine | pH not specified in the publication, 25°C | Solanum pennellii | |
0.65 | - |
L-isoleucine | pH not specified in the publication, 25°C | Solanum pennellii | |
0.67 | - |
L-leucine | pH not specified in the publication, 25°C | Solanum pennellii | |
4.92 | - |
4-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum | |
5.52 | - |
3-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum | |
5.58 | - |
3-methyl-2-oxobutanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum | |
7.09 | - |
3-methyl-2-oxobutanoic acid | pH not specified in the publication, 25°C | Solanum pennellii | |
7.9 | - |
3-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum pennellii | |
11.65 | - |
4-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum pennellii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
cv. M82 | - |
Solanum pennellii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON Affinity Purification Resin or Glutathione-Superflow Resin | Solanum lycopersicum |
TALON Affinity Purification Resin or Glutathione-Superflow Resin | Solanum pennellii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Solanum lycopersicum | - |
fruit | - |
Solanum lycopersicum | - |
leaf | - |
Solanum lycopersicum | - |
shoot | - |
Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.018 | - |
L-isoleucine, pH not specified in the publication, 25°C | Solanum lycopersicum |
0.018 | - |
L-leucine, pH not specified in the publication, 25°C | Solanum lycopersicum |
0.03 | - |
3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C | Solanum pennellii |
0.042 | - |
4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C | Solanum pennellii |
0.066 | - |
3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C | Solanum lycopersicum |
0.066 | - |
L-valine, pH not specified in the publication, 25°C | Solanum lycopersicum |
0.096 | - |
L-leucine, pH not specified in the publication, 25°C | Solanum pennellii |
0.096 | - |
L-valine, pH not specified in the publication, 25°C | Solanum pennellii |
0.114 | - |
L-isoleucine, pH not specified in the publication, 25°C | Solanum pennellii |
0.2 | - |
3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C | Solanum lycopersicum |
0.21 | - |
3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C | Solanum pennellii |
0.41 | - |
4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoic acid + L-glutamate | - |
Solanum lycopersicum | L-valine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxobutanoic acid + L-glutamate | - |
Solanum pennellii | L-valine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxopentanoic acid + L-glutamate | - |
Solanum lycopersicum | L-isoleucine + 2-oxoglutarate | - |
? | |
3-methyl-2-oxopentanoic acid + L-glutamate | - |
Solanum pennellii | L-isoleucine + 2-oxoglutarate | - |
? | |
4-methyl-2-oxopentanoic acid + L-glutamate | - |
Solanum lycopersicum | L-leucine + 2-oxoglutarate | - |
? | |
4-methyl-2-oxopentanoic acid + L-glutamate | - |
Solanum pennellii | L-leucine + 2-oxoglutarate | - |
? | |
L-isoleucine + 2-oxoglutarate | - |
Solanum lycopersicum | L-glutamate + 3-methyl-2-oxopentanoate | - |
? | |
L-isoleucine + 2-oxoglutarate | - |
Solanum pennellii | L-glutamate + 3-methyl-2-oxopentanoate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Solanum lycopersicum | L-glutamate + 4-methyl-2-oxopentanoate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Solanum pennellii | L-glutamate + 4-methyl-2-oxopentanoate | - |
? | |
L-valine + 2-oxoglutarate | - |
Solanum lycopersicum | L-glutamate + 3-methyl-2-oxobutanoate | - |
? | |
L-valine + 2-oxoglutarate | - |
Solanum pennellii | L-glutamate + 3-methyl-2-oxobutanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BCAT7 | - |
Solanum lycopersicum |
BCAT7 | - |
Solanum pennellii |
branched-chain aminotransferase 7 | - |
Solanum lycopersicum |
branched-chain aminotransferase 7 | - |
Solanum pennellii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.6 | - |
L-isoleucine | pH not specified in the publication, 25°C | Solanum lycopersicum | |
8.1 | - |
L-leucine | pH not specified in the publication, 25°C | Solanum lycopersicum | |
11.7 | - |
3-methyl-2-oxobutanoic acid | pH not specified in the publication, 25°C | Solanum pennellii | |
16.3 | - |
4-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum pennellii | |
23.4 | - |
3-methyl-2-oxobutanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum | |
28.1 | - |
L-valine | pH not specified in the publication, 25°C | Solanum lycopersicum | |
39.1 | - |
L-valine | pH not specified in the publication, 25°C | Solanum pennellii | |
40.8 | - |
L-leucine | pH not specified in the publication, 25°C | Solanum pennellii | |
46.5 | - |
L-isoleucine | pH not specified in the publication, 25°C | Solanum pennellii | |
83.9 | - |
3-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum | |
84.8 | - |
3-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum pennellii | |
173.8 | - |
4-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
4-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum pennellii | |
2 | - |
3-methyl-2-oxobutanoic acid | pH not specified in the publication, 25°C | Solanum pennellii | |
5 | - |
3-methyl-2-oxobutanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum | |
11 | - |
3-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum pennellii | |
17 | - |
3-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum | |
25 | - |
L-isoleucine | pH not specified in the publication, 25°C | Solanum lycopersicum | |
38 | - |
4-methyl-2-oxopentanoic acid | pH not specified in the publication, 25°C | Solanum lycopersicum | |
40 | - |
L-leucine | pH not specified in the publication, 25°C | Solanum lycopersicum | |
61 | - |
L-leucine | pH not specified in the publication, 25°C | Solanum pennellii | |
70 | - |
L-valine | pH not specified in the publication, 25°C | Solanum pennellii | |
70 | - |
L-isoleucine | pH not specified in the publication, 25°C | Solanum pennellii | |
80 | - |
L-valine | pH not specified in the publication, 25°C | Solanum lycopersicum |