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Literature summary for 2.6.1.42 extracted from

  • Hong, E.; Cha, M.; Yun, H.; Kim, B.
    Asymmetric synthesis of L-tert-leucine and L-3-hydroxyadamantylglycine using branched chain aminotransferase (2010), J. Mol. Catal. B, 66, 228-233.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21 Escherichia coli
His-tagged protein expressed in Escherichia coli BL21 Enterobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
Enterobacter sp.
2-oxoglutarate
-
Escherichia coli
aminooxyacetic acid
-
Enterobacter sp.
gabaculine
-
Enterobacter sp.
hydroxylamine
-
Enterobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
trimethylpyruvate pH 8.0, 37°C Enterobacter sp.
0.09
-
trimethylpyruvate pH 8.0, 37°C Escherichia coli
0.42
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
0.63
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
0.78
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Enterobacter sp.
0.97
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
0.99
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
1.64
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Escherichia coli
7.38
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Escherichia coli
9.52
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Enterobacter sp.

Organism

Organism UniProt Comment Textmining
Enterobacter sp.
-
-
-
Enterobacter sp. TL3
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Escherichia coli
immobilized metal ion affinity chromatography (Ni2+) Enterobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate
-
Escherichia coli 3-hydroxyadamantylglycine + 2-oxoglutarate
-
?
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate
-
Enterobacter sp. 3-hydroxyadamantylglycine + 2-oxoglutarate
-
?
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate
-
Enterobacter sp. TL3 3-hydroxyadamantylglycine + 2-oxoglutarate
-
?
3-methyl-2-oxobutyric acid + L-glutamate
-
Escherichia coli L-valine + 2-oxoglutarate
-
?
3-methyl-2-oxobutyric acid + L-glutamate
-
Enterobacter sp. L-valine + 2-oxoglutarate
-
?
3-methyl-2-oxobutyric acid + L-glutamate
-
Enterobacter sp. TL3 L-valine + 2-oxoglutarate
-
?
3-methyl-2-oxovaleric acid + L-glutamate
-
Escherichia coli L-isoleucine + 2-oxoglutarate
-
?
3-methyl-2-oxovaleric acid + L-glutamate
-
Enterobacter sp. L-isoleucine + 2-oxoglutarate
-
?
3-methyl-2-oxovaleric acid + L-glutamate
-
Enterobacter sp. TL3 L-isoleucine + 2-oxoglutarate
-
?
4-methyl-2-oxovaleric acid + L-glutamate
-
Escherichia coli L-leucine + 2-oxoglutarate
-
?
4-methyl-2-oxovaleric acid + L-glutamate
-
Enterobacter sp. L-leucine + 2-oxoglutarate
-
?
4-methyl-2-oxovaleric acid + L-glutamate
-
Enterobacter sp. TL3 L-leucine + 2-oxoglutarate
-
?
trimethylpyruvate + L-glutamate
-
Escherichia coli L-tert-leucine + 2-oxoglutarate
-
?
trimethylpyruvate + L-glutamate
-
Enterobacter sp. L-tert-leucine + 2-oxoglutarate
-
?
trimethylpyruvate + L-glutamate
-
Enterobacter sp. TL3 L-tert-leucine + 2-oxoglutarate
-
?

Synonyms

Synonyms Comment Organism
branched chain aminotransferase
-
Escherichia coli
branched chain aminotransferase
-
Enterobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
trimethylpyruvate pH 8.0, 37°C Escherichia coli
2.59
-
trimethylpyruvate pH 8.0, 37°C Enterobacter sp.
2.9
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Enterobacter sp.
4.11
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Escherichia coli
6.61
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
12.15
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
12.45
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Enterobacter sp.
13.75
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
16.84
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Escherichia coli
17.32
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Escherichia coli
7.5
-
-
Enterobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9 58% activity at pH 6.0, 78% activity at pH 9.0 Escherichia coli
6 9 58% activity at pH 6.0, 78% activity at pH 9.0 Enterobacter sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.3
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Enterobacter sp.
0.56
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Escherichia coli
10.27
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Escherichia coli
13.89
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
15.74
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
15.96
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Enterobacter sp.
17.85
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
19.29
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
24.44
-
trimethylpyruvate pH 8.0, 37°C Escherichia coli
43.16
-
trimethylpyruvate pH 8.0, 37°C Enterobacter sp.