Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.42 extracted from

  • Saito, M.; Nishimura, K.; Wakabayashi, S.; Kurihara, T.; Nagata, Y.
    Purification of branched-chain amino acid aminotransferase from Helicobacter pylori NCTC 11637 (2007), Amino Acids, 33, 445-449.
    View publication on PubMed
Search for more literature for 2.6.1.42

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.085
-
 2-oxoglutarate pH 8.0, 37°C Helicobacter pylori
0.085
-
 2-oxoglutarate in 50mM Tris-HCl buffer, pH 8.0, at 37°C Helicobacter pylori
0.34
-
 L-isoleucine pH 8.0, 37°C Helicobacter pylori
0.34
-
 L-isoleucine in 50mM Tris-HCl buffer, pH 8.0, at 37°C Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 37539, calculated, 2 * 36000, SDS-PAGE Helicobacter pylori
37539
-
 2 * 37539, calculated, 2 * 36000, SDS-PAGE Helicobacter pylori
37540
-
 calculated from sequence of cDNA Helicobacter pylori
68500
-
 gel filtration Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-
Helicobacter pylori Q0E9S8
-
-
Helicobacter pylori NCTC 11637
-
-
-
Helicobacter pylori NCTC 11637 Q0E9S8
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Toyopearl column chromatography, Phenyl Sepharose column chromatography, DEAE Sepharose column chromatography, Shodex KW-2003 gel filtration, and Butyl Toyopearl gel filtration Helicobacter pylori

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + 2-oxoglutarate
-
 Helicobacter pylori glyoxylate + L-glutamate 0.4% of the activity with L-isoleucine ?
L-aspartate + 2-oxoglutarate
-
 Helicobacter pylori oxaloacetate + L-glutamate 1.7% of the activity with L-isoleucine ?
L-aspartate + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 oxaloacetate + L-glutamate 1.7% of the activity with L-isoleucine ?
L-isoleucine + 2-oxoglutarate
-
 Helicobacter pylori 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-isoleucine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-leucine + 2-oxoglutarate
-
 Helicobacter pylori 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-leucine + 2-oxoglutarate
-
 Helicobacter pylori 4-methyl-2-oxopentanoate + L-glutamate 70.1% of the activity with L-isoleucine ?
L-leucine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-leucine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 4-methyl-2-oxopentanoate + L-glutamate 70.1% of the activity with L-isoleucine ?
L-methionine + 2-oxoglutarate
-
 Helicobacter pylori 4-methylsulfanyl-2-oxobutanoate + L-glutamate 2.9% of the activity with L-isoleucine ?
L-methionine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 4-methylsulfanyl-2-oxobutanoate + L-glutamate 2.9% of the activity with L-isoleucine ?
L-phenylalanine + 2-oxoglutarate
-
 Helicobacter pylori phenylpyruvate + L-glutamate 0.9% of the activity with L-isoleucine ?
L-phenylalanine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 phenylpyruvate + L-glutamate 0.9% of the activity with L-isoleucine ?
L-valine + 2-oxoglutarate
-
 Helicobacter pylori 3-methyl-2-oxobutanoate + L-glutamate
-
 ?
L-valine + 2-oxoglutarate
-
 Helicobacter pylori 3-methyl-2-oxobutanoate + L-glutamate 47.9% of the activity with L-isoleucine ?
L-valine + 2-oxoglutarate
-
 Helicobacter pylori NCTC 11637 3-methyl-2-oxobutanoate + L-glutamate
-
 ?
additional information no or very weak activity is shown toward L-methionine, L-aspartate, L-phenylalanine, glycine and the D-enantiomers of leucine, isoleucine and valine Helicobacter pylori ?
-
 ?
additional information no substrate: L-asparagine, L-histidine, and L-lysine, D-isoleucine, D-leucine, D-valine, as well as both enantiomers of alanine, proline, and serine Helicobacter pylori ?
-
 ?
additional information no or very weak activity is shown toward L-methionine, L-aspartate, L-phenylalanine, glycine and the D-enantiomers of leucine, isoleucine and valine Helicobacter pylori NCTC 11637 ?
-
 ?
additional information no substrate: L-asparagine, L-histidine, and L-lysine, D-isoleucine, D-leucine, D-valine, as well as both enantiomers of alanine, proline, and serine Helicobacter pylori NCTC 11637 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 37539, calculated, 2 * 36000, SDS-PAGE Helicobacter pylori

Synonyms

Synonyms Comment Organism
BcaT
-
 Helicobacter pylori
branched-chain amino acid aminotransferase
-
 Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Helicobacter pylori

pI Value

Organism Comment pI Value Maximum pI Value
Helicobacter pylori calculated from sequence of cDNA
-
 6.47
Helicobacter pylori calculated
-
 6.5