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Literature summary for 2.6.1.42 extracted from

  • Thage, B.V.; Rattray, F.P.; Laustsen, M.W.; Ardo, Y.; Barkholt, V.; Houlberg, U.
    Purification and characterization of a branched-chain amino acid aminotransferase from Lactobacillus paracasei subsp. paracasei CHCC 2115 (2004), J. Appl. Microbiol., 96, 593-602.
    View publication on PubMed

Application

Application Comment Organism
nutrition use of enzyme for development of flavor in cheese Lacticaseibacillus paracasei

Inhibitors

Inhibitors Comment Organism Structure
carboxymethoxylamine 1 mM, 20% residual activity Lacticaseibacillus paracasei
Co2+ 1 mM, 49% residual activity Lacticaseibacillus paracasei
Cu2+ 1 mM, 39% residual activity Lacticaseibacillus paracasei
Fe2+ 1 mM, 34% residual activity Lacticaseibacillus paracasei
Hg2+ 1 mM, 0% residual activity Lacticaseibacillus paracasei
hydroxylamine 1 mM, 3% residual activity Lacticaseibacillus paracasei
additional information not inhibitory: 4% NaCl, 1 mM iodoacetamide, 1 mM phenylmethylsulfonylfluoride Lacticaseibacillus paracasei
phenylhydrazine 1 mM, 0% residual activity Lacticaseibacillus paracasei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
1 * 41000, SDS-PAGE Lacticaseibacillus paracasei
50000
-
gel filtration Lacticaseibacillus paracasei

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus paracasei
-
subsp. paracasei CHCC 2115
-

Purification (Commentary)

Purification (Comment) Organism
-
Lacticaseibacillus paracasei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.1
-
pH 7.4, 37°C Lacticaseibacillus paracasei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate
-
Lacticaseibacillus paracasei 3-methyl-2-oxopentanoate + L-glutamate
-
?
L-leucine + 2-oxoglutarate 82% of the activity with L-isoleucine Lacticaseibacillus paracasei 4-methyl-2-oxopentanoate + L-glutamate
-
?
L-leucine + 2-oxohexanoic acid 214% of the activity with 2-oxoglutarate Lacticaseibacillus paracasei 4-methyl-2-oxopentanoate + 2-aminohexanoic acid
-
?
L-leucine + 2-oxoisovaleric acid 135% of the activity with 2-oxoglutarate Lacticaseibacillus paracasei 4-methyl-2-oxopentanoate + 2-aminoisovaleric acid
-
?
L-methionine + 2-oxoglutarate 6% of the activity with L-isoleucine Lacticaseibacillus paracasei 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
?
L-valine + 2-oxoglutarate 86% of the activity with L-isoleucine Lacticaseibacillus paracasei 3-methyl-2-oxobutanoate + L-glutamate
-
?
additional information no substrate: phenylalanine, tyrosine, tryptophan, aspartic acid, asparagine Lacticaseibacillus paracasei ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Lacticaseibacillus paracasei
More N-terminal amino acid sequence Lacticaseibacillus paracasei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
Lacticaseibacillus paracasei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
and 4% NaCl, 13% of maximum activity Lacticaseibacillus paracasei
7.3
-
-
Lacticaseibacillus paracasei