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Literature summary for 2.6.1.42 extracted from

  • Cooper, A.J.; Bruschi, S.A.; Conway, M.; Hutson, S.M.
    Human mitochondrial and cytosolic branched-chain aminotransferases are cysteine S-conjugate beta-lyases, but turnover leads to inactivation (2003), Biochem. Pharmacol., 65, 181-192.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant BCATm and BCATc Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-oxoisocaproate
-
Homo sapiens
benzothiazolyl-L-cysteine inhibits the L-leucine-2-oxoglutarate tranamination reaction of both isoenzymes Homo sapiens
beta-chloro-L-alanine rapidly inactivated by the beta-lyase substrate Homo sapiens
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine rapidly inactivated by the beta-lyase substrate Homo sapiens
S-(1,2-dichlorovinyl)-L-cysteine rapidly inactivated by the beta-lyase substrate Homo sapiens
S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine rapidly inactivated by the beta-lyase substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
beta-chloro-L-alanine pH 7.4, 23°C, recombinant BCATm Homo sapiens
8.4
-
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine pH 7.4, 23°C, recombinant BCATm Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
mitochondrion
-
Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41730
-
2 * 41730, recombinant BCATm, homodimer Homo sapiens
43400
-
2 * 43400, recombinant BCATc, homodimer Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloro-L-alanine + ?
-
Homo sapiens 3-chloropyruvate + ?
-
?
additional information recombinant human BCATm and BCATc have beta-lyase activity towards 3 toxic L-cysteine S-conjugates, S-(1,1,2,2-tetrafluoroethyl)-L-cysteine, S-(1,2-dichlorovinyl)-L-cysteine and S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine and toward 3-chloro-L-alanine, BCATm is also active toward benzothiazolyl-L-cysteine, pyruvate formed from beta-lyase substrates Homo sapiens ?
-
?
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine + ?
-
Homo sapiens S-(1,1,2,2-tetrafluoroethyl)-2-oxo-3-thiopropanoate + ?
-
?
S-(1,2-dichlorovinyl)-L-cysteine + ?
-
Homo sapiens S-(1,2-dichlorovinyl)-2-oxo-3-thiopropanoate + ?
-
?
S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine + ?
-
Homo sapiens S-(2-chloro-1,1,2-trifluoroethyl)-2-oxo-3-thiopropanoate + ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43400, recombinant BCATc, homodimer Homo sapiens
dimer 2 * 41730, recombinant BCATm, homodimer Homo sapiens

Synonyms

Synonyms Comment Organism
BCATc
-
Homo sapiens
BCATm
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens