Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
vector pET-28a, overexpression of recombinant BCATm and BCATc in Escherichia coli | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Homo sapiens | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Homo sapiens | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-leucine + 2-oxoglutarate | Rattus norvegicus | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Bcatm and BCATc, expressed in E. coli | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
85 | - |
recombinant cytosolic enzyme | Homo sapiens |
125 | - |
recombinant mitochondrial enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Homo sapiens | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Homo sapiens | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BCATc | - |
Homo sapiens |
BCATc | - |
Rattus norvegicus |
BCATm | - |
Homo sapiens |
BCATm | - |
Rattus norvegicus |