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Literature summary for 2.6.1.42 extracted from

  • Atiles, M.W.; Dudley, E.G.; Steele, J.L.
    Gene cloning, sequencing, and inactivation of the branched-chain aminotransferase of Lactococcus lactis LM0230 (2000), Appl. Environ. Microbiol., 66, 2325-2329.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.42

Cloned(Commentary)

Cloned (Comment) Organism
LM0230 ilvE gene identified by complementation in Escherichia coli DL39 Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Lactococcus lactis
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-isoleucine + 2-oxoglutarate Lactococcus lactis LM0230
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-leucine + 2-oxoglutarate Lactococcus lactis
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate Lactococcus lactis LM0230
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate Lactococcus lactis
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 LM0230
-
Lactococcus lactis LM0230
-
 LM0230
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
 substrate L-phenylalanine Lactococcus lactis
2.4
-
 substrate L-methionine Lactococcus lactis
25
-
 substrate L-valine Lactococcus lactis
27
-
 substrate L-leucine Lactococcus lactis
47
-
 substrate L-isoleucine Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate
-
 Lactococcus lactis 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-isoleucine + 2-oxoglutarate
-
 Lactococcus lactis 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-isoleucine + 2-oxoglutarate
-
 Lactococcus lactis LM0230 3-methyl-2-oxopentanoate + L-glutamate
-
 ?
L-isoleucine + 2-oxoglutarate
-
 Lactococcus lactis LM0230 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate
-
 Lactococcus lactis 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate
-
 Lactococcus lactis LM0230 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-methionine + 2-oxoglutarate
-
 Lactococcus lactis 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 r
L-methionine + 2-oxoglutarate
-
 Lactococcus lactis LM0230 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 r
L-phenylalanine + 2-oxoglutarate
-
 Lactococcus lactis phenylpyruvate + L-glutamate
-
 r
L-phenylalanine + 2-oxoglutarate
-
 Lactococcus lactis LM0230 phenylpyruvate + L-glutamate
-
 r
L-valine + 2-oxoglutarate
-
 Lactococcus lactis 3-methyl-2-oxobutanoate + L-glutamate
-
 r