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Literature summary for 2.6.1.42 extracted from

  • Schadewaldt, P.; Adelmeyer, F.
    Coupled enzymatic assay for estimation of branched-chain L-amino acid aminotransferase activity with 2-Oxo acid substrates (1996), Anal. Biochem., 238, 65-71.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-methyl-2-oxopentanoate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
(R,S)-3-methyl-2-oxopentanoate pH 8.3, 25°C Rattus norvegicus
0.11
-
3-methyl-2-oxobutanoate pH 8.3, 25°C Rattus norvegicus
0.14
-
4-methyl-2-oxopentanoate pH 8.3, 25°C Rattus norvegicus
2.45
-
L-glutamate pH 8.3, 25°C, (R,S)-3-methyl-2-oxopentanoate as amino group acceptor Rattus norvegicus
3.6
-
L-glutamate pH 8.3, 25°C, 3-methyl-2-oxobutanoate as amino group acceptor Rattus norvegicus
6.65
-
L-glutamate pH 8.3, 25°C, 4-methyl-2-oxopentanoate as amino group acceptor Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-leucine + 2-oxoglutarate Rattus norvegicus
-
4-methyl-2-oxopentanoate + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
bovine
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
aorta endothelial cells Bos taurus
-
heart
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine + 2-oxoglutarate
-
Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 4-methyl-2-oxopentanoate
-
Rattus norvegicus 4-methyl-2-oxopentanoate + L-leucine
-
r

Synonyms

Synonyms Comment Organism
BCAA-AT
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1
-
4-methyl-2-oxopentanoate pH 8.3, 25°C Rattus norvegicus