Literature summary for 2.6.1.42 extracted from

Kanda, M.; Hori, K.; Kurotsu, T.; Ohgishi, K.; Hanawa, T.; Saito, Y.
Purification and properties of branched chain amino acid aminotransferase from gramicidin S-producing Bacillus brevis (1995), J. Nutr. Sci. Vitaminol., 41, 51-60.

Search for more literature for 2.6.1.42

Inhibitors

Inhibitors	Comment	Organism	Structure
hydrazine	-	Brevibacillus brevis
hydroxylamine	-	Brevibacillus brevis
phenylhydrazine	-	Brevibacillus brevis
Semicarbazide	-	Brevibacillus brevis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.22	-	L-valine	pH 8.0, 37°C	Brevibacillus brevis
0.25	-	L-leucine	pH 8.0, 37°C	Brevibacillus brevis
0.43	-	L-isoleucine	pH 8.0, 37°C	Brevibacillus brevis
0.56	-	L-glutamate	pH 8.0, 37°C	Brevibacillus brevis
0.77	-	2-oxoglutarate	pH 8.0, 37°C, leucine as amino group donor	Brevibacillus brevis
2	-	L-phenylalanine	pH 8.0, 37°C	Brevibacillus brevis
2.9	-	L-tryptophan	pH 8.0, 37°C	Brevibacillus brevis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	2 * 47000, SDS-PAGE	Brevibacillus brevis
93000	-	gel filtration	Brevibacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-isoleucine + 2-oxoglutarate	Brevibacillus brevis	-	3-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	Brevibacillus brevis	last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-valine + 2-oxoglutarate	Brevibacillus brevis	-	3-methyl-2-oxobutanoate + L-glutamate	-	r

Organism

Organism	UniProt	Comment	Textmining
Brevibacillus brevis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Brevibacillus brevis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
13.8	-	-	Brevibacillus brevis

Storage Stability

Storage Stability	Organism
-20°C, 0.05 M potassium phosphate buffer, pH 7.5, 10% glycerol, 1 mN DTT, 0.1 M pyridoxal 5'-phosphate, purified enzyme can be stored at least for 3 months without loss of activity	Brevibacillus brevis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine + 2-oxoglutarate	-	Brevibacillus brevis	2-oxopropanoate + L-glutamate	-	r
L-aspartate + 2-oxoglutarate	-	Brevibacillus brevis	oxaloacetate + L-glutamate	-	r
L-isoleucine + 2-oxoglutarate	-	Brevibacillus brevis	3-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	-	Brevibacillus brevis	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-leucine + 2-oxoglutarate	last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine	Brevibacillus brevis	4-methyl-2-oxopentanoate + L-glutamate	-	r
L-methionine + 2-oxoglutarate	-	Brevibacillus brevis	4-methylsulfanyl-2-oxobutanoate + L-glutamate	-	r
L-norleucine + 2-oxoglutarate	-	Brevibacillus brevis	2-oxohexanoate + L-glutamate	-	r
L-norvaline + 2-oxoglutarate	-	Brevibacillus brevis	2-oxopentanoate + L-glutamate	-	r
L-phenylalanine + 2-oxoglutarate	-	Brevibacillus brevis	phenylpyruvate + L-glutamate	-	r
L-tryptophan + 2-oxoglutarate	-	Brevibacillus brevis	2-oxo-3-indolylpropanoate + L-glutamate	-	r
L-tyrosine + 2-oxoglutarate	-	Brevibacillus brevis	p-hydroxyphenylpyruvate + L-glutamate	-	r
L-valine + 2-oxoglutarate	-	Brevibacillus brevis	3-methyl-2-oxobutanoate + L-glutamate	-	r
additional information	glycine and D-leucine are no substrates, oxaloacetate, pyruvate and glyoxylate are not amino acceptors	Brevibacillus brevis	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 47000, SDS-PAGE	Brevibacillus brevis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.3	-	-	Brevibacillus brevis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	Km 0.0065	Brevibacillus brevis

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Release 2023.1 (January 2023)