Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | - |
Rattus norvegicus | |
diethyldicarbonate | - |
Rattus norvegicus | |
Mersalyl | - |
Rattus norvegicus | |
N-ethylmaleimide | - |
Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, BCATc, SDS-PAGE | Rattus norvegicus |
47000 | - |
1 * 47000, BCATm, SDS-PAGE | Rattus norvegicus |
91000 | - |
BCATc, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Rattus norvegicus | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mitochondrial and cytosolic isoenzymes | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | - |
Rattus norvegicus | - |
brain | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
hepatocyte | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | negligible activity in adult, high activity in fetal liver | Rattus norvegicus | - |
muscle | mixed | Rattus norvegicus | - |
ovary | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
placenta | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
98.33 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C,stable for several weeks without addition of dithiothreitol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-allo-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-glutamate + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxoglutarate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxo-3-methiobutyrate | - |
Rattus norvegicus | 2-oxoisohexanoate + L-methionine | - |
r | |
L-leucine + 2-oxo-butyrate | - |
Rattus norvegicus | 2-oxoisohexanoate + 2-aminobutyrate | - |
r | |
L-leucine + 2-oxo-hexanoate | - |
Rattus norvegicus | 2-oxoisohexanoate + 2-aminohexanoate | - |
r | |
L-leucine + 2-oxo-pentanoate | - |
Rattus norvegicus | 2-oxoisohexanoate + 2-aminopentanoate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoisohexanoate | - |
Rattus norvegicus | 2-oxoisohexanoate + L-leucine | - |
r | |
L-leucine + 2-oxoisopentanoate | - |
Rattus norvegicus | 2-oxoisohexanoate + L-valine | - |
r | |
L-leucine + DL-2-oxo-3-methylpentanoate | - |
Rattus norvegicus | 2-oxoisohexanoate + L-isoleucine | - |
r | |
L-leucine + phenylpyruvate | 2-oxo-isohexanoic acid 100%, BCATm relative rate 4%, BCATc 6% | Rattus norvegicus | 2-oxoisohexanoate + L-phenylalanine | - |
r | |
L-leucine + pyruvate | 2-oxo-isohexanoic acid 100%, BCATm and BCATc, relative rate 6% | Rattus norvegicus | 2-oxoisohexanoate + L-alanine | - |
r | |
L-methionine + 2-oxoglutarate | transaminated extremely poorly | Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
r | |
L-norleucine + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxohexanoate + L-glutamate | - |
r | |
L-norvaline + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxopentanoate + L-glutamate | - |
r | |
L-threo-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
additional information | only little or no activity with L-tryptophan, L-phenylalanine, L-glutamine, L-alanine and L-aspartate, KIC, KIV, DL-2oxo-3-methylpentanoate, 2-oxoglutarate, 2-oxohexanoate, 2-oxopentanoate, 2-oxobutyrate, 2-oxo-3-methiobutyrate, pyruvate or phenylpyruvate are acceptors | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, BCATm, SDS-PAGE | Rattus norvegicus |
monomer | 1 * 41000, BCATc, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
BCATc | - |
Rattus norvegicus |
BCATm | - |
Rattus norvegicus |