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Literature summary for 2.6.1.42 extracted from

  • Hall, T.R.; Wallin, R.; Reinhart, G.D.; Hutson, S.M.
    Branched chain aminotransferase isoenzymes. Purification and characterization of the rat brain isoenzyme (1993), J. Biol. Chem., 268, 3092-3098.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate
-
Rattus norvegicus
diethyldicarbonate
-
Rattus norvegicus
Mersalyl
-
Rattus norvegicus
N-ethylmaleimide
-
Rattus norvegicus
p-chloromercuribenzoate
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-
mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
1 * 41000, BCATc, SDS-PAGE Rattus norvegicus
47000
-
1 * 47000, BCATm, SDS-PAGE Rattus norvegicus
91000
-
BCATc, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Rattus norvegicus
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate Rattus norvegicus
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate Rattus norvegicus
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mitochondrial and cytosolic isoenzymes Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte
-
Rattus norvegicus
-
brain
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
hepatocyte
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver negligible activity in adult, high activity in fetal liver Rattus norvegicus
-
muscle mixed Rattus norvegicus
-
ovary
-
Rattus norvegicus
-
pancreas
-
Rattus norvegicus
-
placenta
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
98.33
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C,stable for several weeks without addition of dithiothreitol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-allo-isoleucine + 2-oxoglutarate
-
Rattus norvegicus 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-glutamate + 2-oxoglutarate
-
Rattus norvegicus 2-oxoglutarate + L-glutamate
-
r
L-isoleucine + 2-oxoglutarate
-
Rattus norvegicus 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxo-3-methiobutyrate
-
Rattus norvegicus 2-oxoisohexanoate + L-methionine
-
r
L-leucine + 2-oxo-butyrate
-
Rattus norvegicus 2-oxoisohexanoate + 2-aminobutyrate
-
r
L-leucine + 2-oxo-hexanoate
-
Rattus norvegicus 2-oxoisohexanoate + 2-aminohexanoate
-
r
L-leucine + 2-oxo-pentanoate
-
Rattus norvegicus 2-oxoisohexanoate + 2-aminopentanoate
-
r
L-leucine + 2-oxoglutarate
-
Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoisohexanoate
-
Rattus norvegicus 2-oxoisohexanoate + L-leucine
-
r
L-leucine + 2-oxoisopentanoate
-
Rattus norvegicus 2-oxoisohexanoate + L-valine
-
r
L-leucine + DL-2-oxo-3-methylpentanoate
-
Rattus norvegicus 2-oxoisohexanoate + L-isoleucine
-
r
L-leucine + phenylpyruvate 2-oxo-isohexanoic acid 100%, BCATm relative rate 4%, BCATc 6% Rattus norvegicus 2-oxoisohexanoate + L-phenylalanine
-
r
L-leucine + pyruvate 2-oxo-isohexanoic acid 100%, BCATm and BCATc, relative rate 6% Rattus norvegicus 2-oxoisohexanoate + L-alanine
-
r
L-methionine + 2-oxoglutarate transaminated extremely poorly Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
r
L-norleucine + 2-oxoglutarate
-
Rattus norvegicus 2-oxohexanoate + L-glutamate
-
r
L-norvaline + 2-oxoglutarate
-
Rattus norvegicus 2-oxopentanoate + L-glutamate
-
r
L-threo-isoleucine + 2-oxoglutarate
-
Rattus norvegicus 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate
-
Rattus norvegicus 3-methyl-2-oxobutanoate + L-glutamate
-
r
additional information only little or no activity with L-tryptophan, L-phenylalanine, L-glutamine, L-alanine and L-aspartate, KIC, KIV, DL-2oxo-3-methylpentanoate, 2-oxoglutarate, 2-oxohexanoate, 2-oxopentanoate, 2-oxobutyrate, 2-oxo-3-methiobutyrate, pyruvate or phenylpyruvate are acceptors Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47000, BCATm, SDS-PAGE Rattus norvegicus
monomer 1 * 41000, BCATc, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
BCATc
-
Rattus norvegicus
BCATm
-
Rattus norvegicus