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Literature summary for 2.6.1.42 extracted from

  • Lee-Peng, F.C.; Hermodson, M.A.; Kohlhaw, G.B.
    Transaminase B from Escherichia coli: quaternary structure, amino-terminal sequence, substrate specificity, and absence of a separate valine-alpha-ketoglutarate activity (1979), J. Bacteriol., 139, 339-345.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
K-12 strain carries the ilvE gene both on the host chromosome and on a plasmid Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
DL-2-oxo-3-methyl-n-pentanoate pH 8.0, 37°C Escherichia coli
0.52
-
L-isoleucine pH 8.0, 37°C Escherichia coli
0.56
-
2-Oxoisohexanoate pH 8.0, 37°C Escherichia coli
0.56
-
2-oxoisopentanoate pH 8.0, 37°C Escherichia coli
0.58
-
L-leucine pH 8.0, 37°C Escherichia coli
1.28
-
2-oxoglutarate pH 8.0, 37°C Escherichia coli
3.13
-
L-valine pH 8.0, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31500
-
6 * 31500, SDS-PAGE Escherichia coli
182300
-
sedimentation equilibrium centrifugation Escherichia coli
185000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Escherichia coli
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate Escherichia coli
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate Escherichia coli
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.9
-
substrate L-valine + 2-oxoglutarate Escherichia coli
27.3
-
substrate L-isoleucine + 2-oxoglutarate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate
-
Escherichia coli 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate
-
Escherichia coli 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-phenylalanine + 2-oxoglutarate
-
Escherichia coli phenylpyruvate + L-glutamate
-
r
L-tyrosine + 2-oxoglutarate
-
Escherichia coli p-hydroxyphenylpyruvate + L-glutamate
-
r
L-valine + 2-oxoglutarate
-
Escherichia coli 3-methyl-2-oxobutanoate + L-glutamate
-
r
additional information purified enzyme has no measurable L-aspartate-2-oxoglutarate activity Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 31500, SDS-PAGE Escherichia coli