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Literature summary for 2.6.1.42 extracted from

  • Koide, Y.; Honma, M.; Shimomura, T.
    Branched chain amino acid aminotransferase of Pseudomonas sp. (1977), Agric. Biol. Chem., 41, 1171-1177.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
2-oxoisocaproate
-
Pseudomonas sp.
Isocaproic acid competitive inhibitor Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
2-oxoglutarate pH 8.0, 30°C Pseudomonas sp.
0.3
-
L-leucine pH 8.0, 30°C Pseudomonas sp.
1.1
-
2-Oxoisohexanoate pH 8.0, 30°C Pseudomonas sp.
1.2
-
L-leucine pH 8.0, 30°C, activated with beta-mercaptoethanol Pseudomonas sp.
3.2
-
L-glutamate pH 8.0, 30°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-leucine + 2-oxoglutarate Pseudomonas sp.
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate Pseudomonas sp.
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutyrate + 2-oxo-isopentanoate
-
Pseudomonas sp. 2-oxobutanoate + L-valine
-
r
L-2-aminobutyrate + 2-oxoglutarate
-
Pseudomonas sp. 2-oxobutanoate + L-glutamate
-
r
L-2-aminobutyrate + pyruvate
-
Pseudomonas sp. 2-oxobutanoate + L-alanine
-
r
L-alanine + 2-oxoglutarate
-
Pseudomonas sp. 2-oxopropanoate + L-glutamate
-
r
L-arginine + 2-oxoglutarate
-
Pseudomonas sp. 2-oxo-5-guanidinopentanoate + L-glutamate
-
r
L-aspartate + 2-oxoglutarate
-
Pseudomonas sp. oxaloacetate + L-glutamate
-
r
L-glutamate + 2-oxo-isohexanoate
-
Pseudomonas sp. 2-oxoglutarate + L-leucine
-
r
L-glutamate + 2-oxo-isopentanoate
-
Pseudomonas sp. 2-oxoglutarate + L-valine
-
r
L-glutamate + 2-oxobutyrate
-
Pseudomonas sp. 2-oxoglutarate + 2-aminobutyrate
-
r
L-glutamate + 2-oxoglutarate
-
Pseudomonas sp. 2-oxoglutarate + L-glutamate
-
r
L-glutamate + pyruvate
-
Pseudomonas sp. 2-oxoglutarate + L-alanine
-
r
L-histidine + 2-oxoglutarate
-
Pseudomonas sp. 2-oxo-3-imidazolpropanoate + L-glutamate
-
r
L-isoleucine + 2-oxo-isopentanoate
-
Pseudomonas sp. 2-oxoisohexanoate + L-valine
-
r
L-isoleucine + 2-oxobutyrate
-
Pseudomonas sp. 2-oxoisohexanoate + 2-aminobutyrate
-
r
L-isoleucine + 2-oxoglutarate lower reactivity than other origins Pseudomonas sp. 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-isoleucine + 2-oxoisohexanoate
-
Pseudomonas sp. 3-methyl-2-oxopentanoate + L-leucine
-
r
L-isoleucine + pyruvate
-
Pseudomonas sp. 2-oxoisohexanoate + L-alanine
-
r
L-leucine + 2-oxo-butyrate
-
Pseudomonas sp. 2-oxoisohexanoate + 2-aminobutyrate
-
r
L-leucine + 2-oxoglutarate
-
Pseudomonas sp. 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoisohexanoate
-
Pseudomonas sp. 2-oxoisohexanoate + L-leucine
-
r
L-leucine + pyruvate
-
Pseudomonas sp. 2-oxoisohexanoate + L-alanine
-
r
L-lysine + 2-oxoglutarate
-
Pseudomonas sp. 2-oxo-6-aminohexanoate + L-glutamate
-
r
L-methionine + 2-oxo-isohexanoate
-
Pseudomonas sp. 4-methylsulfanyl-2-oxobutanoate + L-leucine
-
r
L-methionine + 2-oxobutyrate
-
Pseudomonas sp. 4-methylsulfanyl-2-oxobutanoate + 2-aminobutyrate
-
r
L-methionine + 2-oxoglutarate higher reactivity than other origins Pseudomonas sp. 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
r
L-methionine + pyruvate
-
Pseudomonas sp. 4-methylsulfanyl-2-oxobutanoate + L-alanine
-
r
L-phenylalanine + 2-oxoglutarate
-
Pseudomonas sp. phenylpyruvate + L-glutamate
-
r
L-serine + 2-oxoglutarate
-
Pseudomonas sp. 3-hydroxy-2-oxopropanoate + L-glutamate
-
r
L-threonine + 2-oxoglutarate
-
Pseudomonas sp. 2-oxo-3-hydroxybutyrate + L-glutamate
-
r
L-tryptophan + 2-oxoglutarate
-
Pseudomonas sp. 2-oxo-3-indolylpropanoate + L-glutamate
-
r
L-valine + 2-oxo-isohexanoate
-
Pseudomonas sp. 2-oxoisopentanoate + L-leucine
-
r
L-valine + 2-oxobutyrate
-
Pseudomonas sp. 2-oxoisopentanoate + 2-aminobutanoate
-
r
L-valine + 2-oxoglutarate
-
Pseudomonas sp. 3-methyl-2-oxobutanoate + L-glutamate
-
r
L-valine + pyruvate
-
Pseudomonas sp. 2-oxoisopentanoate + L-alanine
-
r
additional information L-glutamine is no substrate Pseudomonas sp. ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.8
-
alpha-Ketoisocaproate pH 8.0, 30°C Pseudomonas sp.
6
-
Isocaproic acid unactivated enzyme Pseudomonas sp.
14
-
Isocaproic acid activated enzyme Pseudomonas sp.