Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1.0 mM, relative activity 101% | Gluconobacter oxydans | |
alpha,alpha'-dipyridyl | 1.0 mM, relative activity 103% | Gluconobacter oxydans | |
EDTA | 1.0 mM, relative activity 108% | Gluconobacter oxydans | |
glutathione | 1.0 mM, relative activity 109% | Gluconobacter oxydans | |
neocuproin | 1.0 mM, relative activity 106% | Gluconobacter oxydans | |
o-phenanthroline | 1.0 mM, relative activity 108% | Gluconobacter oxydans | |
thioglycolate | 1.0 mM, relative activity 101% | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
Gluconobacter oxydans | |
hydroxylamine | - |
Gluconobacter oxydans | |
p-chloromercuribenzoate | - |
Gluconobacter oxydans | |
phenylhydrazine | - |
Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
2-oxoisopentanoate | pH 8.0, 37°C | Gluconobacter oxydans | |
0.91 | - |
2-Oxoisohexanoate | pH 8.0, 37°C | Gluconobacter oxydans | |
1.7 | - |
L-norleucine | pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor | Gluconobacter oxydans | |
1.82 | - |
L-leucine | pH 8.0, 37°C | Gluconobacter oxydans | |
2.5 | - |
2-oxo-3-methylpentanoate | pH 8.0, 37°C | Gluconobacter oxydans | |
3.03 | - |
L-isoleucine | pH 8.0, 37°C | Gluconobacter oxydans | |
4.57 | - |
2-oxoglutarate | pH 8.0, 37°C, leucine as amino group donor | Gluconobacter oxydans | |
4.7 | - |
L-norvaline | pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor | Gluconobacter oxydans | |
5.72 | - |
2-oxoglutarate | pH 8.0, 37°C, valine as amino group donor | Gluconobacter oxydans | |
6.45 | - |
L-valine | pH 8.0, 37°C, 2-oxoglutarate as amino group acceptor | Gluconobacter oxydans | |
6.67 | - |
2-oxoglutarate | pH 8.0, 37°C, isoleucine as amino group donor | Gluconobacter oxydans | |
10 | - |
L-aspartate | pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor | Gluconobacter oxydans | |
16.7 | - |
glutamate | pH 8.0, 37°C, leucine as amino group donor | Gluconobacter oxydans | |
18.2 | - |
L-glutamate | pH 8.0, 37°C, isoleucine as amino group donor | Gluconobacter oxydans | |
25 | - |
L-glutamate | pH 8.0, 37°C, valine as amino group donor | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
sedimentation equilibrium | Gluconobacter oxydans |
82000 | - |
gel filtration | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Gluconobacter oxydans | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Gluconobacter oxydans | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Gluconobacter oxydans | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
IFO 3172, synonym Gluconobacter suboxydans | - |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42.8 | - |
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminobutyrate + 2-oxoglutarate | - |
Gluconobacter oxydans | 2-oxobutanoate + L-glutamate | - |
r | |
L-asparagine + 2-oxoglutarate | - |
Gluconobacter oxydans | 2,4-dioxo-4-aminobutanoate + L-glutamate | - |
r | |
L-aspartate + 4-methyl-2-oxopentanoate | - |
Gluconobacter oxydans | ? | - |
? | |
L-glutamine + 2-oxoglutarate | - |
Gluconobacter oxydans | 2,5-dioxo-5-aminopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Gluconobacter oxydans | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Gluconobacter oxydans | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + pyruvate | 2-oxoglutarate 100%, relative rate 1% | Gluconobacter oxydans | 2-oxoisohexanoate + L-alanine | - |
r | |
L-methionine + 2-oxoglutarate | - |
Gluconobacter oxydans | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
r | |
L-norleucine + 2-oxoglutarate | - |
Gluconobacter oxydans | 2-oxohexanoate + L-glutamate | - |
r | |
L-norvaline + 2-oxoglutarate | - |
Gluconobacter oxydans | 2-oxopentanoate + L-glutamate | - |
r | |
L-phenylalanine + 2-oxoglutarate | - |
Gluconobacter oxydans | phenylpyruvate + L-glutamate | - |
r | |
L-tryptophan + 2-oxoglutarate | - |
Gluconobacter oxydans | 2-oxo-3-indolylpropanoate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | - |
Gluconobacter oxydans | p-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Gluconobacter oxydans | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
additional information | L-aspartic acid, L-arginine, L-citrulline, L-lysine, L-ornithine, L-alanine, beta-alanine and gamma-aminobutyrate are not amino donors, oxalaceteate and glyoxylate are not amino acceptors, pyruvate is a weak amino acceptor, relative activity 1% | Gluconobacter oxydans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-KGA | - |
Gluconobacter oxydans |
L-leucine-alpha-ketoglutarate transaminase | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | stable at 0-30°C, rapidly inactivated over 40°C at pH 6.0 | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | most stable in this range | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | pyridoxine 5'-phosphate, pyridoxal, pyridoxamine and pyridoxine are not effective as coenzymes | Gluconobacter oxydans | |
pyridoxal 5'-phosphate | Km 0.00053 mM | Gluconobacter oxydans | |
pyridoxamine 5'-phosphate | Km 0.00062 mM | Gluconobacter oxydans |