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Literature summary for 2.6.1.42 extracted from

  • Tachiki, T.; Tochikura, T.
    Purification and characterization of L-leucine-alpha-ketoglutarate transaminase from Acetobacter suboxydans (1976), Agric. Biol. Chem., 40, 2187-2192.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1.0 mM, relative activity 101% Gluconobacter oxydans
alpha,alpha'-dipyridyl 1.0 mM, relative activity 103% Gluconobacter oxydans
EDTA 1.0 mM, relative activity 108% Gluconobacter oxydans
glutathione 1.0 mM, relative activity 109% Gluconobacter oxydans
neocuproin 1.0 mM, relative activity 106% Gluconobacter oxydans
o-phenanthroline 1.0 mM, relative activity 108% Gluconobacter oxydans
thioglycolate 1.0 mM, relative activity 101% Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
Gluconobacter oxydans
hydroxylamine
-
Gluconobacter oxydans
p-chloromercuribenzoate
-
Gluconobacter oxydans
phenylhydrazine
-
Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
2-oxoisopentanoate pH 8.0, 37°C Gluconobacter oxydans
0.91
-
2-Oxoisohexanoate pH 8.0, 37°C Gluconobacter oxydans
1.7
-
L-norleucine pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor Gluconobacter oxydans
1.82
-
L-leucine pH 8.0, 37°C Gluconobacter oxydans
2.5
-
2-oxo-3-methylpentanoate pH 8.0, 37°C Gluconobacter oxydans
3.03
-
L-isoleucine pH 8.0, 37°C Gluconobacter oxydans
4.57
-
2-oxoglutarate pH 8.0, 37°C, leucine as amino group donor Gluconobacter oxydans
4.7
-
L-norvaline pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor Gluconobacter oxydans
5.72
-
2-oxoglutarate pH 8.0, 37°C, valine as amino group donor Gluconobacter oxydans
6.45
-
L-valine pH 8.0, 37°C, 2-oxoglutarate as amino group acceptor Gluconobacter oxydans
6.67
-
2-oxoglutarate pH 8.0, 37°C, isoleucine as amino group donor Gluconobacter oxydans
10
-
L-aspartate pH 8.3, 37°C, 4-methyl-2-oxopentanoate as amino group acceptor Gluconobacter oxydans
16.7
-
glutamate pH 8.0, 37°C, leucine as amino group donor Gluconobacter oxydans
18.2
-
L-glutamate pH 8.0, 37°C, isoleucine as amino group donor Gluconobacter oxydans
25
-
L-glutamate pH 8.0, 37°C, valine as amino group donor Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
sedimentation equilibrium Gluconobacter oxydans
82000
-
gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Gluconobacter oxydans
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate Gluconobacter oxydans
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate Gluconobacter oxydans
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
IFO 3172, synonym Gluconobacter suboxydans
-

Purification (Commentary)

Purification (Comment) Organism
-
Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.8
-
-
Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutyrate + 2-oxoglutarate
-
Gluconobacter oxydans 2-oxobutanoate + L-glutamate
-
r
L-asparagine + 2-oxoglutarate
-
Gluconobacter oxydans 2,4-dioxo-4-aminobutanoate + L-glutamate
-
r
L-aspartate + 4-methyl-2-oxopentanoate
-
Gluconobacter oxydans ?
-
?
L-glutamine + 2-oxoglutarate
-
Gluconobacter oxydans 2,5-dioxo-5-aminopentanoate + L-glutamate
-
r
L-isoleucine + 2-oxoglutarate
-
Gluconobacter oxydans 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate
-
Gluconobacter oxydans 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + pyruvate 2-oxoglutarate 100%, relative rate 1% Gluconobacter oxydans 2-oxoisohexanoate + L-alanine
-
r
L-methionine + 2-oxoglutarate
-
Gluconobacter oxydans 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
r
L-norleucine + 2-oxoglutarate
-
Gluconobacter oxydans 2-oxohexanoate + L-glutamate
-
r
L-norvaline + 2-oxoglutarate
-
Gluconobacter oxydans 2-oxopentanoate + L-glutamate
-
r
L-phenylalanine + 2-oxoglutarate
-
Gluconobacter oxydans phenylpyruvate + L-glutamate
-
r
L-tryptophan + 2-oxoglutarate
-
Gluconobacter oxydans 2-oxo-3-indolylpropanoate + L-glutamate
-
r
L-tyrosine + 2-oxoglutarate
-
Gluconobacter oxydans p-hydroxyphenylpyruvate + L-glutamate
-
r
L-valine + 2-oxoglutarate
-
Gluconobacter oxydans 3-methyl-2-oxobutanoate + L-glutamate
-
r
additional information L-aspartic acid, L-arginine, L-citrulline, L-lysine, L-ornithine, L-alanine, beta-alanine and gamma-aminobutyrate are not amino donors, oxalaceteate and glyoxylate are not amino acceptors, pyruvate is a weak amino acceptor, relative activity 1% Gluconobacter oxydans ?
-
?

Synonyms

Synonyms Comment Organism
alpha-KGA
-
Gluconobacter oxydans
L-leucine-alpha-ketoglutarate transaminase
-
Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
40 stable at 0-30°C, rapidly inactivated over 40°C at pH 6.0 Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 6 most stable in this range Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
additional information pyridoxine 5'-phosphate, pyridoxal, pyridoxamine and pyridoxine are not effective as coenzymes Gluconobacter oxydans
pyridoxal 5'-phosphate Km 0.00053 mM Gluconobacter oxydans
pyridoxamine 5'-phosphate Km 0.00062 mM Gluconobacter oxydans