Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.42 extracted from

  • Aki, K.; Yokojima, A.; Ichihara, A.
    Transaminase of branched chain amino acids. VI. Purification and properties of the hog brain enzyme (1969), J. Biochem., 65, 539-544.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
L-leucine pH 8.2, 37°C, brain enzyme Sus scrofa
0.57
-
2-oxoglutarate pH 8.2, 37°C, brain enzyme Sus scrofa
0.67
-
L-isoleucine pH 8.2, 37°C, brain enzyme Sus scrofa
1.4
-
L-valine pH8.2, 37°C, brain enzyme Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Sus scrofa 5737
-
mitochondrion
-
Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
calculated from S20 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Sus scrofa
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate Sus scrofa
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate Sus scrofa
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
pig, hog
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Sus scrofa
-
heart
-
Sus scrofa
-
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7273
-
brain enzyme, substrate L-leucine Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate
-
Sus scrofa 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-isoleucine + oxaloacetate brain enzyme Sus scrofa 3-methyl-2-oxopentanoate + L-aspartate
-
r
L-leucine + 2-oxoglutarate
-
Sus scrofa 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-norleucine + 2-oxoglutarate brain enzyme Sus scrofa 2-oxohexanoate + L-glutamate
-
r
L-norvaline + 2-oxoglutarate brain enzyme Sus scrofa 2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate
-
Sus scrofa 3-methyl-2-oxobutanoate + L-glutamate
-
r

Subunits

Subunits Comment Organism
monomer
-
Sus scrofa

Synonyms

Synonyms Comment Organism
EC 2.6.1.6 related Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
brain enzyme Sus scrofa
8.7
-
heart enzyme Sus scrofa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Sus scrofa