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Literature summary for 2.6.1.42 extracted from

  • Aki, K.; Ogawa, K.; Ichihara, A.
    Transaminases of branched chain amino acids. IV. Purification and properties of two enzymes from rat liver (1968), Biochim. Biophys. Acta, 159, 276-284.
    View publication on PubMed
Search for more literature for 2.6.1.42

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Rattus norvegicus
2-mercaptoethanol
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 L-leucine pH 8.2, 37°C Rattus norvegicus
0.84
-
 L-isoleucine pH 8.2, 37°C Rattus norvegicus
1
-
 2-oxoglutarate pH 8.2, 37°C Rattus norvegicus
4.3
-
 L-valine pH 8.2, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-
cytoplasm
-
 Sus scrofa 5737
-
microsome
-
 Rattus norvegicus
-
-
mitochondrion
-
 Sus scrofa 5739
-
mitochondrion mainly located in the cytoplasm Rattus norvegicus 5739
-
nucleus
-
 Rattus norvegicus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Rattus norvegicus
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-isoleucine + 2-oxoglutarate Sus scrofa
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate Sus scrofa
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate Rattus norvegicus sole transaminase in fetal rat liver 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate Rattus norvegicus
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate Sus scrofa
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r
additional information Rattus norvegicus specific for L-leucine, L-isoleucine or L-valine, no other amino acid would serve as an amino donor ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 rat, strain Wistar
-
Sus scrofa
-
 pig, hog
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01855
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate
-
 Rattus norvegicus 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-isoleucine + 2-oxoglutarate
-
 Sus scrofa 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate
-
 Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate
-
 Sus scrofa 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate sole transaminase in fetal rat liver Rattus norvegicus 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate
-
 Rattus norvegicus 3-methyl-2-oxobutanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate
-
 Sus scrofa 3-methyl-2-oxobutanoate + L-glutamate
-
 r
additional information specific for L-leucine, L-isoleucine or L-valine, no other amino acid would serve as an amino donor Rattus norvegicus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km 0.025 mM Rattus norvegicus
pyridoxal 5'-phosphate Km 0.0065 mM Sus scrofa