BRENDA - Enzyme Database show
show all sequences of 2.6.1.41

An aminotransferase specific for the D-enantiomorph of methionine

Durham, J.I.; Morgan, P.W.; Prescott, J.M.; Lyman, C.M.; Phytochemistry 12, 2123-2126 (1973)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
pyridoxal 5'-phosphate stabilizes
Arachis hypogaea
reducing agents, such as cysteine, 2-mercaptoethanol or dithiothreitol, are essential for maintaining activity during purification
Arachis hypogaea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arachis hypogaea
-
peanut cv. Starr
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, cold precipitation, acetone precipitation, partial purification
Arachis hypogaea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
embryo
germinating
Arachis hypogaea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
10061 units/mg, 1 unit is defined as the quantity of enzyme that produces 1 nanoliter of ethylene per h of dark reaction
Arachis hypogaea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-methionine + 2-oxoglutarate
less effective than pyruvate
636703
Arachis hypogaea
4-methylthio-2-oxobutanoate + L-glutamate
-
636703
Arachis hypogaea
?
D-methionine + oxaloacetate
less effective than pyruvate
636703
Arachis hypogaea
4-methylthio-2-oxobutanoate + L-aspartate
-
636703
Arachis hypogaea
?
D-methionine + pyruvate
-
636703
Arachis hypogaea
4-methylthio-2-oxobutanoate + L-alanine
-
636703
Arachis hypogaea
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
31
-
assay at
Arachis hypogaea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Arachis hypogaea
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required for full enzyme activity
Arachis hypogaea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required for full enzyme activity
Arachis hypogaea
General Stability (protein specific)
General Stability
Organism
pyridoxal 5'-phosphate stabilizes
Arachis hypogaea
reducing agents, such as cysteine, 2-mercaptoethanol or dithiothreitol, are essential for maintaining activity during purification
Arachis hypogaea
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, cold precipitation, acetone precipitation, partial purification
Arachis hypogaea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
embryo
germinating
Arachis hypogaea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
10061 units/mg, 1 unit is defined as the quantity of enzyme that produces 1 nanoliter of ethylene per h of dark reaction
Arachis hypogaea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-methionine + 2-oxoglutarate
less effective than pyruvate
636703
Arachis hypogaea
4-methylthio-2-oxobutanoate + L-glutamate
-
636703
Arachis hypogaea
?
D-methionine + oxaloacetate
less effective than pyruvate
636703
Arachis hypogaea
4-methylthio-2-oxobutanoate + L-aspartate
-
636703
Arachis hypogaea
?
D-methionine + pyruvate
-
636703
Arachis hypogaea
4-methylthio-2-oxobutanoate + L-alanine
-
636703
Arachis hypogaea
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
31
-
assay at
Arachis hypogaea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Arachis hypogaea
Other publictions for EC 2.6.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
636703
Durham
-
An aminotransferase specific f ...
Arachis hypogaea
Phytochemistry
12
2123-2126
1973
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
1
1
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
636704
Mapson
Biosynthesis of ethylene. 4-me ...
Brassica sp.
Biochem. J.
115
653-661
1969
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
1
-
-
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-