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show all sequences of 2.6.1.40

Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver

Tamaki, N.; Sakata, S.F.; Matsuda, K.; Methods Enzymol. 324, 376-389 (2000)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-fluoro-beta-alanine
-
Rattus norvegicus
gabaculine
-
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
4 * 52000, SDS-PAGE
Rattus norvegicus
220000
-
gel filtration
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
assay principle
Rattus norvegicus
11.3
-
pH 8.8, 37C
Rattus norvegicus
Storage Stability
Storage Stability
Organism
4C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-amino-2-methylpropanoate + glyoxylate
with 90% efficiency compared to pyruvate
636964
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
r
(R)-3-amino-2-methylpropanoate + oxaloacetate
with 63% efficiency compared to pyruvate
636964
Rattus norvegicus
2-methyl-3-oxopropanoate + L-aspartate
-
-
-
r
beta-alanine + pyruvate
transamination at 66% the rate of (R)-3-amino-2-methylpropanoate
636964
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
additional information
overview
636964
Rattus norvegicus
?
-
-
-
-
additional information
no substrate: 2-oxoglutarate
636964
Rattus norvegicus
?
-
-
-
-
additional information
gamma-aminobutanoate is neither substrate nor inhibitor
636964
Rattus norvegicus
?
-
-
-
-
additional information
no substrate: phenylpyruvate
636964
Rattus norvegicus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 52000, SDS-PAGE
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
gamma-aminobutanoate is neither substrate nor inhibitor
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-fluoro-beta-alanine
-
Rattus norvegicus
gabaculine
-
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
gamma-aminobutanoate is neither substrate nor inhibitor
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
4 * 52000, SDS-PAGE
Rattus norvegicus
220000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
assay principle
Rattus norvegicus
11.3
-
pH 8.8, 37C
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
4C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-amino-2-methylpropanoate + glyoxylate
with 90% efficiency compared to pyruvate
636964
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
r
(R)-3-amino-2-methylpropanoate + oxaloacetate
with 63% efficiency compared to pyruvate
636964
Rattus norvegicus
2-methyl-3-oxopropanoate + L-aspartate
-
-
-
r
beta-alanine + pyruvate
transamination at 66% the rate of (R)-3-amino-2-methylpropanoate
636964
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
additional information
overview
636964
Rattus norvegicus
?
-
-
-
-
additional information
no substrate: 2-oxoglutarate
636964
Rattus norvegicus
?
-
-
-
-
additional information
gamma-aminobutanoate is neither substrate nor inhibitor
636964
Rattus norvegicus
?
-
-
-
-
additional information
no substrate: phenylpyruvate
636964
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 52000, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Rattus norvegicus
Other publictions for EC 2.6.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737947
Abe
Distribution of D-3-aminoisobu ...
Rattus norvegicus
BMC Neurosci.
15
53
2014
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1
-
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2
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3
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-
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-
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-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636964
Tamaki
Purification, properties, and ...
Rattus norvegicus
Methods Enzymol.
324
376-389
2000
-
-
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-
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-
2
-
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-
2
-
-
1
-
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1
-
-
1
2
1
7
1
-
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-
-
1
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-
1
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-
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-
2
1
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2
-
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-
1
-
1
2
1
7
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
644431
Kontani
Identity of D-3-aminoisobutyra ...
Rattus norvegicus
Biochim. Biophys. Acta
1156
161-166
1993
-
-
-
-
-
-
11
-
-
-
1
-
-
2
-
-
-
1
-
2
-
-
2
-
1
-
-
-
-
-
-
1
-
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-
-
-
-
1
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-
-
-
11
-
-
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-
1
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-
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-
-
2
-
-
2
-
1
-
-
-
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-
-
-
-
-
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-
-
-
637062
Kaneko
Inhibition of D-3-aminoisobuty ...
Rattus norvegicus
Biochim. Biophys. Acta
1122
45-49
1992
-
-
-
-
-
-
12
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
12
-
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-
-
-
-
-
-
-
-
-
2
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637049
Kaneko
Irreversible inhibition of D-3 ...
Rattus norvegicus
FEBS Lett.
276
115-118
1990
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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-
1
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-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
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-
-
1
-
-
-
-
-
-
-
-
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-
1
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1
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-
644426
Tamaki
Purification, characterization ...
Rattus norvegicus
Eur. J. Biochem.
189
39-45
1990
-
-
-
-
-
-
6
2
1
-
2
-
-
2
-
-
1
-
-
3
-
1
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
2
1
-
2
-
-
-
-
1
-
3
-
1
9
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
644430
Ueno
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
1033
169-175
1990
2
-
-
-
-
-
2
-
1
-
2
-
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2
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1
-
-
2
1
2
12
1
1
-
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1
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2
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1
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-
-
2
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1
-
2
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1
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2
1
2
12
1
1
-
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-
-
-
-
-
-
-
-
-
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644428
Kakimoto
D-beta-Aminoisobutyrate:pyruva ...
Cavia porcellus, Homo sapiens, Rattus norvegicus, Sus scrofa
J. Biol. Chem.
244
335-340
1969
2
-
-
-
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-
1
1
-
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-
4
-
4
-
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1
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7
1
1
19
-
4
-
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1
1
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2
-
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2
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2
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-
-
1
-
1
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-
-
4
-
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1
-
7
1
1
19
-
4
-
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-
1
1
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-
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