BRENDA - Enzyme Database
show all sequences of 2.6.1.40

Purification and characterization of D-3-aminoisobutyrate-pyruvate aminotransferase from rat liver

Ueno, S.I.; Morino, H.; Sano, A.; Kakimoto, Y.; Biochim. Biophys. Acta 1033, 169-175 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation only in the presence of pyridoxal phosphate
Rattus norvegicus
DTT
activation
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
GSH
weak
Rattus norvegicus
L-cysteine
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
4 * 50000, reductive SDS-PAGE
Rattus norvegicus
180000
-
gel filtration
Rattus norvegicus
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.14
-
pH 8.8, 37C
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20C, concentrated enzyme solution, in 5% polyethylene glycol plus 1 mg/ml pyridoxal 5'-phosphate, several months
Rattus norvegicus
4C, concentrated enzyme solution, several days
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-3-amino-2-methylpropanoate + 2-oxo-n-butanoate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + 2-amino-n-butanoate
-
-
-
?
(R)-3-amino-2-methylpropanoate + 2-oxo-n-pentanoate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + norvaline
-
-
-
?
(R)-3-amino-2-methylpropanoate + glyoxylate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
?
(R)-3-amino-2-methylpropanoate + oxaloacetate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + L-aspartate
-
-
-
?
(R)-3-amino-2-methylpropanoate + pyruvate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
-
644430
Rattus norvegicus
?
5-aminolevulinic acid + pyruvate
-
644430
Rattus norvegicus
5-oxolevulinic acid + L-alanine
-
-
-
?
arginine + pyruvate
transamination at 40% the rate of (R)-3-amino-2-methylpropanoate
644430
Rattus norvegicus
2-oxo-5-guanidinovaleric acid + L-alanine
-
-
-
?
beta-alanine + pyruvate
-
644430
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
DL-3-amino-n-butanoate + pyruvate
-
644430
Rattus norvegicus
3-oxobutanoate + L-alanine
-
-
-
?
glycine + pyruvate
-
644430
Rattus norvegicus
glyoxylate + L-alanine
-
-
-
?
additional information
overview
644430
Rattus norvegicus
?
-
-
-
-
NG,NG'-dimethyl-(S)-arginine + pyruvate
-
644430
Rattus norvegicus
? + L-alanine
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 50000, reductive SDS-PAGE
Rattus norvegicus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement, tightly bound
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation only in the presence of pyridoxal phosphate
Rattus norvegicus
DTT
activation
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement, tightly bound
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
GSH
weak
Rattus norvegicus
L-cysteine
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
4 * 50000, reductive SDS-PAGE
Rattus norvegicus
180000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.14
-
pH 8.8, 37C
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20C, concentrated enzyme solution, in 5% polyethylene glycol plus 1 mg/ml pyridoxal 5'-phosphate, several months
Rattus norvegicus
4C, concentrated enzyme solution, several days
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-3-amino-2-methylpropanoate + 2-oxo-n-butanoate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + 2-amino-n-butanoate
-
-
-
?
(R)-3-amino-2-methylpropanoate + 2-oxo-n-pentanoate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + norvaline
-
-
-
?
(R)-3-amino-2-methylpropanoate + glyoxylate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
?
(R)-3-amino-2-methylpropanoate + oxaloacetate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + L-aspartate
-
-
-
?
(R)-3-amino-2-methylpropanoate + pyruvate
-
644430
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
-
644430
Rattus norvegicus
?
5-aminolevulinic acid + pyruvate
-
644430
Rattus norvegicus
5-oxolevulinic acid + L-alanine
-
-
-
?
arginine + pyruvate
transamination at 40% the rate of (R)-3-amino-2-methylpropanoate
644430
Rattus norvegicus
2-oxo-5-guanidinovaleric acid + L-alanine
-
-
-
?
beta-alanine + pyruvate
-
644430
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
DL-3-amino-n-butanoate + pyruvate
-
644430
Rattus norvegicus
3-oxobutanoate + L-alanine
-
-
-
?
glycine + pyruvate
-
644430
Rattus norvegicus
glyoxylate + L-alanine
-
-
-
?
additional information
overview
644430
Rattus norvegicus
?
-
-
-
-
NG,NG'-dimethyl-(S)-arginine + pyruvate
-
644430
Rattus norvegicus
? + L-alanine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 50000, reductive SDS-PAGE
Rattus norvegicus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Other publictions for EC 2.6.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737947
Abe
Distribution of D-3-aminoisobu ...
Rattus norvegicus
BMC Neurosci.
15
53
2014
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636964
Tamaki
Purification, properties, and ...
Rattus norvegicus
Methods Enzymol.
324
376-389
2000
-
-
-
-
-
-
2
-
-
-
2
-
-
1
-
-
1
-
-
1
2
1
7
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
2
-
-
-
-
1
-
1
2
1
7
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
644431
Kontani
Identity of D-3-aminoisobutyra ...
Rattus norvegicus
Biochim. Biophys. Acta
1156
161-166
1993
-
-
-
-
-
-
11
-
-
-
1
-
-
2
-
-
-
1
-
2
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637062
Kaneko
Inhibition of D-3-aminoisobuty ...
Rattus norvegicus
Biochim. Biophys. Acta
1122
45-49
1992
-
-
-
-
-
-
12
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637049
Kaneko
Irreversible inhibition of D-3 ...
Rattus norvegicus
FEBS Lett.
276
115-118
1990
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
644426
Tamaki
Purification, characterization ...
Rattus norvegicus
Eur. J. Biochem.
189
39-45
1990
-
-
-
-
-
-
6
2
1
-
2
-
-
2
-
-
1
-
-
3
-
1
9
1
-
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
2
1
-
2
-
-
-
-
1
-
3
-
1
9
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
644430
Ueno
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
1033
169-175
1990
2
-
-
-
-
-
2
-
1
-
2
-
-
2
-
-
1
-
-
2
1
2
12
1
-
1
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
2
-
-
1
-
2
-
-
-
-
1
-
2
1
2
12
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
644428
Kakimoto
D-beta-Aminoisobutyrate:pyruva ...
Cavia porcellus, Homo sapiens, Rattus norvegicus, Sus scrofa
J. Biol. Chem.
244
335-340
1969
2
-
-
-
-
-
1
1
-
-
-
4
-
4
-
-
1
-
-
7
1
1
19
-
-
4
-
-
-
1
1
-
2
-
-
-
2
-
-
2
-
-
-
-
1
-
1
-
-
-
4
-
-
-
1
-
7
1
1
19
-
4
-
-
-
1
1
-
-
-
-
-
-
-
-