BRENDA - Enzyme Database
show all sequences of 2.6.1.40

D-beta-Aminoisobutyrate:pyruvate aminotransferase in mammalian liver and excretion of beta-aminoisobutyrate by man

Kakimoto, Y.; Taniguchi, K.; Sano, I.; J. Biol. Chem. 244, 335-340 (1969)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation only in the presence of pyridoxal phosphate
Rattus norvegicus
2-mercaptoethanol
activation only in the presence of pyridoxal phosphate
Sus scrofa
Inhibitors
Inhibitors
Commentary
Organism
Structure
hydroxylamine
-
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.81
-
beta-Alanine
pH 8.8, 37C
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-3-amino-2-methylpropanoate + pyruvate
Cavia porcellus
first step in aminoisobutyrate metabolism
2-methyl-3-oxopropanoate + L-alanine
-
Cavia porcellus
-
(R)-3-amino-2-methylpropanoate + pyruvate
Homo sapiens
first step in aminoisobutyrate metabolism
2-methyl-3-oxopropanoate + L-alanine
-
Homo sapiens
-
(R)-3-amino-2-methylpropanoate + pyruvate
Rattus norvegicus
first step in aminoisobutyrate metabolism
2-methyl-3-oxopropanoate + L-alanine
-
Rattus norvegicus
-
(R)-3-amino-2-methylpropanoate + pyruvate
Sus scrofa
first step in aminoisobutyrate metabolism
2-methyl-3-oxopropanoate + L-alanine
-
Sus scrofa
-
Organism
Organism
UniProt
Commentary
Textmining
Cavia porcellus
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Cavia porcellus
-
liver
-
Rattus norvegicus
-
liver
-
Cavia porcellus
-
liver
-
Homo sapiens
-
liver
-
Sus scrofa
-
additional information
distribution in guinea pig tissue
Cavia porcellus
-
additional information
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.093
-
pH 8.8, 37C
Sus scrofa
Storage Stability
Storage Stability
Organism
-20C, at least 1 month
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-3-amino-2-methylpropanoate + glyoxylate
-
644428
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
?
(R)-3-amino-2-methylpropanoate + glyoxylate
with 50% efficiency compared to pyruvate
644428
Sus scrofa
2-methyl-3-oxopropanoate + glycine
-
-
-
?
(R)-3-amino-2-methylpropanoate + pyruvate
-
644428
Homo sapiens
2-methyl-3-oxopropanoate + L-alanine
-
644428
Homo sapiens
-
(R)-3-amino-2-methylpropanoate + pyruvate
-
644428
Sus scrofa
2-methyl-3-oxopropanoate + L-alanine
-
644428
Sus scrofa
-
(R)-3-amino-2-methylpropanoate + pyruvate
-
644428
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
i.e. methylmalonyl semialdehyde
644428
Rattus norvegicus
-
(R)-3-amino-2-methylpropanoate + pyruvate
best substrate among omega-amino acids, highly specific for D-isomer
644428
Cavia porcellus
2-methyl-3-oxopropanoate + L-alanine
-
644428
Cavia porcellus
r
(R)-3-amino-2-methylpropanoate + pyruvate
first step in aminoisobutyrate metabolism
644428
Cavia porcellus
2-methyl-3-oxopropanoate + L-alanine
-
644428
Cavia porcellus
-
(R)-3-amino-2-methylpropanoate + pyruvate
first step in aminoisobutyrate metabolism
644428
Homo sapiens
2-methyl-3-oxopropanoate + L-alanine
-
644428
Homo sapiens
-
(R)-3-amino-2-methylpropanoate + pyruvate
first step in aminoisobutyrate metabolism
644428
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
-
644428
Rattus norvegicus
-
(R)-3-amino-2-methylpropanoate + pyruvate
first step in aminoisobutyrate metabolism
644428
Sus scrofa
2-methyl-3-oxopropanoate + L-alanine
-
644428
Sus scrofa
-
4-aminobutanoate + pyruvate
transamination at 19% the rate of (R)-3-amino-2-methylpropanoate
644428
Sus scrofa
succinic semialdehyde + L-alanine
-
-
-
?
beta-alanine + pyruvate
-
644428
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
beta-alanine + pyruvate
transamination at 66% the rate of (R)-3-amino-2-methylpropanoate
644428
Sus scrofa
malonic semialdehyde + L-alanine
-
-
-
?
DL-alpha-ethyl-beta-alanine + pyruvate
transamination at 33% the rate of (R)-3-amino-2-methylpropanoate
644428
Sus scrofa
2-ethyl-3-oxopropanoate + L-alanine
-
-
-
?
additional information
-
644428
Cavia porcellus
?
-
-
-
-
additional information
-
644428
Homo sapiens
?
-
-
-
-
additional information
no substrate: 2-oxoglutarate
644428
Rattus norvegicus
?
-
-
-
-
additional information
no substrate: 2-oxoglutarate
644428
Sus scrofa
?
-
-
-
-
additional information
no substrate: 5-amino-n-pentanoate, 6-aminohexanoate, DL-3-aminobutyrate, oxaloacetate
644428
Sus scrofa
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Cavia porcellus
37
-
assay at
Homo sapiens
37
-
assay at
Rattus norvegicus
37
-
assay at
Sus scrofa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Sus scrofa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
10.6
about half-maximal activity at pH 7.5 and pH 10.6
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Rattus norvegicus
pyridoxal 5'-phosphate
-
Sus scrofa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation only in the presence of pyridoxal phosphate
Rattus norvegicus
2-mercaptoethanol
activation only in the presence of pyridoxal phosphate
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Rattus norvegicus
pyridoxal 5'-phosphate
-
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydroxylamine
-
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.81
-
beta-Alanine
pH 8.8, 37C
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-3-amino-2-methylpropanoate + pyruvate
Cavia porcellus
first step in aminoisobutyrate metabolism
2-methyl-3-oxopropanoate + L-alanine
-
Cavia porcellus
-
(R)-3-amino-2-methylpropanoate + pyruvate
Homo sapiens
first step in aminoisobutyrate metabolism
2-methyl-3-oxopropanoate + L-alanine
-
Homo sapiens
-
(R)-3-amino-2-methylpropanoate + pyruvate
Rattus norvegicus
first step in aminoisobutyrate metabolism
2-methyl-3-oxopropanoate + L-alanine
-
Rattus norvegicus
-
(R)-3-amino-2-methylpropanoate + pyruvate
Sus scrofa
first step in aminoisobutyrate metabolism
2-methyl-3-oxopropanoate + L-alanine
-
Sus scrofa
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Cavia porcellus
-
liver
-
Rattus norvegicus
-
liver
-
Cavia porcellus
-
liver
-
Homo sapiens
-
liver
-
Sus scrofa
-
additional information
distribution in guinea pig tissue
Cavia porcellus
-
additional information
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.093
-
pH 8.8, 37C
Sus scrofa
Storage Stability (protein specific)
Storage Stability
Organism
-20C, at least 1 month
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-3-amino-2-methylpropanoate + glyoxylate
-
644428
Rattus norvegicus
2-methyl-3-oxopropanoate + glycine
-
-
-
?
(R)-3-amino-2-methylpropanoate + glyoxylate
with 50% efficiency compared to pyruvate
644428
Sus scrofa
2-methyl-3-oxopropanoate + glycine
-
-
-
?
(R)-3-amino-2-methylpropanoate + pyruvate
-
644428
Homo sapiens
2-methyl-3-oxopropanoate + L-alanine
-
644428
Homo sapiens
-
(R)-3-amino-2-methylpropanoate + pyruvate
-
644428
Sus scrofa
2-methyl-3-oxopropanoate + L-alanine
-
644428
Sus scrofa
-
(R)-3-amino-2-methylpropanoate + pyruvate
-
644428
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
i.e. methylmalonyl semialdehyde
644428
Rattus norvegicus
-
(R)-3-amino-2-methylpropanoate + pyruvate
best substrate among omega-amino acids, highly specific for D-isomer
644428
Cavia porcellus
2-methyl-3-oxopropanoate + L-alanine
-
644428
Cavia porcellus
r
(R)-3-amino-2-methylpropanoate + pyruvate
first step in aminoisobutyrate metabolism
644428
Cavia porcellus
2-methyl-3-oxopropanoate + L-alanine
-
644428
Cavia porcellus
-
(R)-3-amino-2-methylpropanoate + pyruvate
first step in aminoisobutyrate metabolism
644428
Homo sapiens
2-methyl-3-oxopropanoate + L-alanine
-
644428
Homo sapiens
-
(R)-3-amino-2-methylpropanoate + pyruvate
first step in aminoisobutyrate metabolism
644428
Rattus norvegicus
2-methyl-3-oxopropanoate + L-alanine
-
644428
Rattus norvegicus
-
(R)-3-amino-2-methylpropanoate + pyruvate
first step in aminoisobutyrate metabolism
644428
Sus scrofa
2-methyl-3-oxopropanoate + L-alanine
-
644428
Sus scrofa
-
4-aminobutanoate + pyruvate
transamination at 19% the rate of (R)-3-amino-2-methylpropanoate
644428
Sus scrofa
succinic semialdehyde + L-alanine
-
-
-
?
beta-alanine + pyruvate
-
644428
Rattus norvegicus
malonic semialdehyde + L-alanine
-
-
-
?
beta-alanine + pyruvate
transamination at 66% the rate of (R)-3-amino-2-methylpropanoate
644428
Sus scrofa
malonic semialdehyde + L-alanine
-
-
-
?
DL-alpha-ethyl-beta-alanine + pyruvate
transamination at 33% the rate of (R)-3-amino-2-methylpropanoate
644428
Sus scrofa
2-ethyl-3-oxopropanoate + L-alanine
-
-
-
?
additional information
-
644428
Cavia porcellus
?
-
-
-
-
additional information
-
644428
Homo sapiens
?
-
-
-
-
additional information
no substrate: 2-oxoglutarate
644428
Rattus norvegicus
?
-
-
-
-
additional information
no substrate: 2-oxoglutarate
644428
Sus scrofa
?
-
-
-
-
additional information
no substrate: 5-amino-n-pentanoate, 6-aminohexanoate, DL-3-aminobutyrate, oxaloacetate
644428
Sus scrofa
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Cavia porcellus
37
-
assay at
Homo sapiens
37
-
assay at
Rattus norvegicus
37
-
assay at
Sus scrofa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Sus scrofa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
10.6
about half-maximal activity at pH 7.5 and pH 10.6
Sus scrofa
Other publictions for EC 2.6.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737947
Abe
Distribution of D-3-aminoisobu ...
Rattus norvegicus
BMC Neurosci.
15
53
2014
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636964
Tamaki
Purification, properties, and ...
Rattus norvegicus
Methods Enzymol.
324
376-389
2000
-
-
-
-
-
-
2
-
-
-
2
-
-
1
-
-
1
-
-
1
2
1
7
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
2
-
-
-
-
1
-
1
2
1
7
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
644431
Kontani
Identity of D-3-aminoisobutyra ...
Rattus norvegicus
Biochim. Biophys. Acta
1156
161-166
1993
-
-
-
-
-
-
11
-
-
-
1
-
-
2
-
-
-
1
-
2
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637062
Kaneko
Inhibition of D-3-aminoisobuty ...
Rattus norvegicus
Biochim. Biophys. Acta
1122
45-49
1992
-
-
-
-
-
-
12
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637049
Kaneko
Irreversible inhibition of D-3 ...
Rattus norvegicus
FEBS Lett.
276
115-118
1990
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
644426
Tamaki
Purification, characterization ...
Rattus norvegicus
Eur. J. Biochem.
189
39-45
1990
-
-
-
-
-
-
6
2
1
-
2
-
-
2
-
-
1
-
-
3
-
1
9
1
-
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
2
1
-
2
-
-
-
-
1
-
3
-
1
9
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
644430
Ueno
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
1033
169-175
1990
2
-
-
-
-
-
2
-
1
-
2
-
-
2
-
-
1
-
-
2
1
2
12
1
-
1
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
2
-
-
1
-
2
-
-
-
-
1
-
2
1
2
12
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
644428
Kakimoto
D-beta-Aminoisobutyrate:pyruva ...
Cavia porcellus, Homo sapiens, Rattus norvegicus, Sus scrofa
J. Biol. Chem.
244
335-340
1969
2
-
-
-
-
-
1
1
-
-
-
4
-
4
-
-
1
-
-
7
1
1
19
-
-
4
-
-
-
1
1
-
2
-
-
-
2
-
-
2
-
-
-
-
1
-
1
-
-
-
4
-
-
-
1
-
7
1
1
19
-
4
-
-
-
1
1
-
-
-
-
-
-
-
-